The cloning and characterization of a cDNA encoding a cytochrome P450 from the flowers of Petunia hybrida

Toguri, Toshihiro; Azuma, Mizue; Ohtani, Takeshi

doi:10.1016/0168-9452(93)90013-p

Cited by 23 publications

(21 citation statements)

References 11 publications

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“…2. TG1 shares 98% homology with a lisianthus clone (E. grandi£orum) [8], 77% with a gentian clone (Gentiana tri£ora) [7], 74% with petunia Hf1 [4,17], 73% with petunia Hf2 [4], and 71% with egg plant (Solanum melongena) [5]. Although E. russellianum and E. grandi£orum are not strictly distinguished systematically, we found signi¢cant genetic variation between TG1 and a previously reported lisianthus clone: there are nine substitutions of amino acid residues.…”

Section: Resultsmentioning

confidence: 52%

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Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

et al. 1999

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Flavonoid-3P P,5P P-hydroxylase (F3P P5P PH), a member of the cytochrome P450 family, is the key enzyme in the synthesis of 3P P,5P P-hydroxylated anthocyanins, which are generally required for blue or purple flowers. A full-length cDNA, TG1, was isolated from prairie gentian by heterologous hybridization with a petunia cDNA, AK14, which encodes F3P P5P PH. To investigate the in vivo function of TG1 and AK14, they were subcloned into a plant expression vector and expressed under the control of the CaMV35S promoter in transgenic tobacco or petunia, both of which originally lack the enzyme. Transgenic petunia plants had a dramatic change in flower color from pink to magenta with a high content of 3P P,5P P-hydroxylated anthocyanins. In contrast, transgenic tobacco plants had minimal color change with at most 35% 3P P,5P P-hydroxylated anthocyanin content. These results indicate that the products of TG1 and AK14 have F3P P5P PH activity in planta and that interspecific gene transfer alters anthocyanin pigment synthesis. The difference in apparent F3P P5P PH activity between tobacco and petunia is discussed.z 1999 Federation of European Biochemical Societies.

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Section: Resultsmentioning

confidence: 52%

“…They detected F3P5PH activity in vitro using a yeast expression system but presented a limited transgenic study. Sequences have also been reported for petunia [5], egg plant [6], gentian [7] and lisianthus [8]. Tanaka et al [7] also reported F3P5PH activity using a yeast expression system.…”

Section: Introductionmentioning

confidence: 88%

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

et al. 1999

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“…[3][4][5][6][7][8][9][10][11] The proline-proline-glycine-proline (PPGP) motif, which is proposed to serve as a hinge between the membrane anchor and the globular part of P450, 19) was observed near the N-terminus of KAP (data not shown). In addition, KAP was structurally similar to known F3?5?Hs ( Fig.…”

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confidence: 99%

“…3,4) as well as by others. [5][6][7][8][9][10][11] Their sequence similarity indicates that they belong to the same gene family of cytochrome P450, CYP75A. Some reviews referred to unpublished results or to the possibility of engineering of anthocyanin composition and ‰ower color with F3?5?H genes.…”

mentioning

confidence: 99%

Selective Accumulation of Delphinidin Derivatives in Tobacco Using a Putative Flavonoid 3′,5′-Hydroxylase cDNA fromCampanula medium

Okinaka

Shimada

Nakano-Shimada

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…X70824) (Toguri et al 1993b) and Petunia hybrida (GenBank accession nos. Z22544, Z22545, and X71130) (Holton et al 1993;Toguri et al 1993a). The gene-specific primers for 39-RACE [no.…”

Section: Methodsmentioning

confidence: 99%

Miniature Inverted-Repeat Transposable Elements ofStowawayAre Active in Potato

2010

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Miniature inverted-repeat transposable elements (MITEs) are dispersed in large numbers within the genomes of eukaryotes although almost all are thought to be inactive. Plants have two major groups of such MITEs: Tourist and Stowaway. Mobile MITEs have been reported previously in rice but no active MITEs have been found in dicotyledons. Here, we provide evidence that Stowaway MITEs can be mobilized in the potato and that one of them causes a change of tuber skin color as an obvious phenotypic variation. In an original red-skinned potato clone, the gene encoding for a flavonoid 39,59-hydroxylase, which is involved in purple anthocyanin synthesis, has been inactivated by the insertion of a Stowaway MITE named dTstu1 within the first exon. However, dTstu1 is absent from this gene in a purple somaclonal variant that was obtained as a regenerated plant from a protoplast culture of the red-skinned potato. The color change was attributed to reversion of flavonoid 39,59-hydroxylase function by removal of dTstu1 from the gene. In this purple variant another specific transposition event has occurred involving a MITE closely related to dTstu1. Instead of being fossil elements, Stowaway MITEs, therefore, still have the ability to become active under particular conditions as represented by tissue culturing.

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The cloning and characterization of a cDNA encoding a cytochrome P450 from the flowers of Petunia hybrida

Cited by 23 publications

References 11 publications

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Selective Accumulation of Delphinidin Derivatives in Tobacco Using a Putative Flavonoid 3′,5′-Hydroxylase cDNA fromCampanula medium

Miniature Inverted-Repeat Transposable Elements ofStowawayAre Active in Potato

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The cloning and characterization of a cDNA encoding a cytochrome P450 from the flowers of Petunia hybrida

Cited by 23 publications

References 11 publications

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants1

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants1

Selective Accumulation of Delphinidin Derivatives in Tobacco Using a Putative Flavonoid 3′,5′-Hydroxylase cDNA fromCampanula medium

Miniature Inverted-Repeat Transposable Elements ofStowawayAre Active in Potato

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Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹