The cloning and characterization of theCDC50 gene family inSaccharomyces cerevisiae

Abstract

We have cloned a gene that complements the cold‐sensitive growth of cdc50‐1 mutant strain of Saccharomyces cerevisiae at 14°C. The CDC50 gene was found to be identical to YCR094w on chromosome III and contains 1173 nucleotides encoding 391 amino acids. We found a missense mutation at the first initiation codon of cdc50‐1. The disruption of the CDC50 gene revealed that it is not essential for growth, but the disruptant caused the same cold‐sensitive phenotype as cdc50‐1, suggesting that the cdc50‐1 is a null mutation resulted from the mutation in the first codon. The cdc50‐1 mutant arrests at START in G1 phase at the non‐permissive temperature. The CDC50 gene product has strong structural similarity to two other proteins in Saccharomyces cerevisiae encoded by YNR048w and YNL323w. The over‐expression of either YNR048w or YNL323w suppressed the cdc50‐1 mutant and the double disruption of either CDC50 and YNR048w or CDC50 and YNL323w resulted in a severe slow‐growth phenotype. We conclude that these three genes constitute a family with redundant function. We also found that the CDC39 gene was a multicopy suppressor of cdc50‐1 mutation, suggesting that the CDC50 family is involved in regulation of transcription via CDC39. Copyright © 2000 John Wiley & Sons, Ltd.

The G2 DNA Damage Checkpoint Delays Expression of Genes Encoding Mitotic Regulators

“…Only one gene was found to be up-regulated by 2 h after mock irradiation. The protein encoded by this gene (EST AA046674/Unigene ID Hs.108350) has significant homology with three predicted membrane proteins of unknown function in budding yeast, Cdc50, YNRO48w, and YNK323w (45,46). Although this gene with homology to Cdc50 was the only gene observed to be up-regulated by 3 h after release from the G 1 /S block, many more genes (53) were found to be induced by 7 h when cells were in the late G 2 phase of cell cycle (Table I).…”

Mutator genes for suppression of gross chromosomal rearrangements identified by a genome-wide screening in Saccharomyces cerevisiae

“…CDC50 is a cell-cycle protein that is localized mainly in the cytoplasm (39) and involved in protein maturation and transport. MMS and hydroxyurea sensitivities conferred on yeast cells by a cdc50⌬ mutation suggest a possible role for CDC50 in DNA metabolism linked to cell-cycle regulation.…”

On the molecular mechanism of flippase- and scramblase-mediated phospholipid transport

“…In yeast, the Cdc50 family consists of 3 members, namely Cdc50p, Lem3p and Crf1p [108], which associate with Drs2p, Dnf1p/Dnf2p and Dnf3p, respectively. The human genome also contains three genes encoding CDC50 proteins: CDC50A, CDC50B and CDC50C.…”