The clinical value of the prostatic exosomal protein expression in the diagnosis of chronic prostatitis: a single-center study

Feng, Xingliang; Zhang, Meng; Zhang, Ligang; Hu, Huaqing; Zhang, Li; Zhang, Xiansheng; Fan, Song; Liang, Chaozhao

doi:10.1007/s11255-019-02313-z

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…The methods for the diagnosis of CP/CPPS mainly depend on the symptoms of patients, and biomarkers used for clinical diagnosis are very limited. Recently, several biomarkers in expressed prostatic secretion (EPS), urine, semen, or serum have been proposed for the diagnosis of CP/CPPS ( 7 , 38 – 40 ). Levels of urinary prostatic exosomal protein have been suggested as a novel biomarker for the diagnosis for CP/CPPS and can be used as a sign of the severity of CP/CPPS ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

Hua

Zhang

et al. 2021

Front. Immunol.

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Chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS) is an inflammatory immune disease characterized by intraprostatic leukocyte infiltration and pelvic or perineal pain. Macrophages play vital roles in the pathogenesis of CP/CPPS. However, the mechanisms controlling the activation and chemotaxis of macrophages in CP/CPPS remain unclear. This study aimed to investigate the roles of the CXCL10/CXCR3 pathway in the activation and chemotaxis of macrophages in CP/CPPS patients. The serums of CP/CPPS patients and healthy volunteers were collected and measured. Results showed that CXCL10 expression was significantly elevated and correlated with the severity of CP/CPPS patients. The experimental autoimmune prostatitis (EAP) model was generated, and adeno-associated virus and CXCR3 inhibitors were used to treat EAP mice. Immunofluorescence, flow cytometry, and Western blotting were used to analyze the functional phenotype and regulation mechanism of macrophages. Results showed that CXCL10 deficiency ameliorates EAP severity by inhibiting infiltration of macrophages to prostate. Moreover, CXCL10 could induce macrophage migrations and secretions of proinflammatory mediators via CXCR3, which consequently activated the downstream Erk1/2 and p38 MAPK signaling pathways. We also showed that prostatic stromal cell is a potential source of CXCL10. Our results indicated CXCL10 as an important mediator involved in inflammatory infiltration and pain symptoms of prostatitis by promoting the migration of macrophages and secretion of inflammatory mediators via CXCR3-mediated ERK and p38 MAPK activation.

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Section: Discussionmentioning

confidence: 99%

Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

Hua

Zhang

et al. 2021

Front. Immunol.

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“…In particular, total antioxidant capacity (T-AOC) is high, and the main product of oxygen free radicals is malondialdehyde (MDA). The change of its content can often reflect the degree of lipid peroxidation in the body and indirectly reflect the degree of cell damage [ 38 ]. A study [ 39 ] found that CP/CPPS rats were injected intracutaneously with CFA combined with prostaglandin purification solution, the T-AOC level in the prostate of the rat decreased, and the MDA level increased.…”

Section: The Mechanism Of Acupuncture and Moxibustion On Cp/cppsmentioning

confidence: 99%

Mechanism of Acupuncture and Moxibustion on Chronic Prostatitis/Chronic Pelvic Pain Syndrome: A Narrative Review of Animal Studies

Cheng

et al. 2021

Pain Research and Management

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Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a genitourinary disease commonly seen in males, with symptoms involving pelvic pain, urinary system disease, and sexual dysfunction, which seriously affects physical and mental health, and it also influences the quality of life of patients. At present, the disease’s aetiology and pathogenesis are unclear, and there is also no effective treatment for it. Acupuncture and moxibustion have been a way to CP/CPPS, showing good curative effect with advantages of safety and affordability. However, the relevant research in this field is less discussed. By adopting databases, such as CNKI, VIP, Wanfang, PubMed, and Medline, this review article used keywords including chronic prostatitis, chronic pelvic pain syndrome, and electric acupuncture, manual acupuncture, moxibustion, and animal experiments, rats, mice, and mechanism research and reviewing research papers published from 1998 to 2021. Then, it further summarized and evaluated the mechanism research and gave a brief comment about modeling methods, acupoints selection, and stimulus parameters that have been used in the selected research papers. Equally important, this review article proposes a reference for the in-depth study of the mechanism of acupuncture and moxibustion on CP/CPPS and provides a theoretical basis to better treat the disease in the clinic.

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“…Therefore, there is an urgent need to find biomarkers and detection methods that are non‐invasive, stable, simple, fast, accurate, and reliable. In recent years, the detection of prostatic exosomal protein (PSEP) in the urine has been reported in the clinical diagnosis of CP, 2,6 but since its diagnostic efficiency and application value are not completely clear, the study of urine PSEP and HSP70 in CP patients has not been reported. In this study, we measured and compared the levels of PSEP and HSP70 in the urine of patients with different types of CP and in control subjects and analyzed their role in the diagnosis and classification of chronic prostatitis.…”

Section: Introductionmentioning

confidence: 99%

The diagnostic value of urine heat shock protein 70 and prostatic exosomal protein in chronic prostatitis

Tang

Pan

Liu

et al. 2021

Clinical Laboratory Analysis

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Objective To explore the diagnostic value of the levels of prostatic exosomal protein (PSEP) and heat shock protein 70 (HSP70) in the urine of patients with chronic prostatitis (CP). Method Urine samples from 210 CP patients (70 cases of the USA National Institutes of Health Category II [NIH‐II], 70 NIH‐IIIa, and 70 NIH‐IIIb patients) and 70 control subjects were collected between May 2018 and February 2020. The levels of PSEP and HSP70 in urine were detected by enzyme‐linked immunosorbent assay. The differences in urine PSEP and HSP70 levels between the groups were analyzed, and receiver operating characteristic (ROC) curves were used to analyze the clinical value of PSEP and HSP70 in the diagnosis of CP. Results The PSEP levels of CP patients were significantly higher than those of the control group (p < 0.001), but there was no difference in PSEP levels among CP subgroups. The level of HSP70 in the urine of the NIH‐II patients was significantly lower than the levels in the NIH‐IIIa and NIH‐IIIb subgroups and the control group, but there was no difference in HSP70 levels between the NIH‐IIIa and NIH‐IIIb subgroups and the control group. ROC curve analysis results showed that the area under the curve (AUC) of PSEP for the NIH‐II, NIH‐IIIa, and NIH‐IIIb patients was 0.751, 0.776, and 0.731, respectively. The AUC of HSP70 in NIH‐II patients was 0.784, and the AUC of combined detection of PSEP and HSP70 in NIH‐II patients was 0.858. Conclusion Urine PSEP can be used as a marker for the diagnosis of CP, but it cannot distinguish between the various types of CP, and HSP70 can be used as a diagnostic index for NIH‐II classification.

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The clinical value of the prostatic exosomal protein expression in the diagnosis of chronic prostatitis: a single-center study

Cited by 3 publications

References 22 publications

Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

Mechanism of Acupuncture and Moxibustion on Chronic Prostatitis/Chronic Pelvic Pain Syndrome: A Narrative Review of Animal Studies

The diagnostic value of urine heat shock protein 70 and prostatic exosomal protein in chronic prostatitis

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