The clinical value of miR‐193a‐3p in non‐small cell lung cancer and its potential molecular mechanism explored in silico using RNA‐sequencing and microarray data

Abstract

miR‐193a‐3p is a tumor‐related miRNA playing an essential role in tumorigenesis and progression of non‐small cell lung cancer (NSCLC). The objective of the present study was to investigate the relationship between miR‐193a‐3p expression and clinical value and to further explore the potential signaling of miR‐193a‐3p in the carcinogenesis of NSCLC. RNA‐sequencing and microarray data were collected from the databases GEO, ArrayExpress and The Cancer Genome Atlas (TCGA). Furthermore, in silico assessments were performed to analyze the prospective pathways and networks of the target genes of miR‐193a‐3p. In total, 453 cases of NSCLC patients and 476 normal controls were included in blood samples, while 920 cases of NSCLC patients and 406 normal controls were included in tissue samples. The pooled positive likelihood ratio, the pooled negative likelihood ratio and the pooled diagnostic odds ratio were calculated to reflect the diagnostic value of miR‐193a‐3p in blood and tissue samples. Moreover, the areas under the curve of the summary receiver operating characteristic curve of blood and tissue were 0.64 and 0.79, respectively. In addition, we found a lower level of miR‐193a in NSCLC tissues than in non‐cancerous controls based on TCGA. A gene ontology (GO) enrichment analysis demonstrated that miR‐193a‐3p could be related to key signaling pathways in NSCLC. Also, several vital pathways were illustrated by KEGG. Lower expression of miR‐193a‐3p in tissue samples of NSCLC may be associated with tumorigenesis and be a predictor of deterioration of NSCLC patients, and pathway analysis revealed crucial signaling pathways correlated with the incidence and progress of NSCLC.

Mechanisms of Compound Kushen Injection for the Treatment of Lung Cancer Based on Network Pharmacology

“…Lung cancer (LC) has become an important etiology of malignant mortality worldwide [1]. Approximately 1.37 million deaths annually worldwide have been attributed to LC, and LC is a significant cause of cancer-related deaths in China [2, 3]. LC is divided into several different categories, such as lung adenocarcinoma, squamous cell lung carcinoma, large cell carcinoma and small cell lung carcinoma (SCLC) [4].…”

Identification of a putative competitive endogenous RNA network for lung adenocarcinoma using TCGA datasets

“…A previously published study has identified abnormally expressed genes in LUAD based on TCGA data (Deng et al, 2018). Significantly altered microRNAs (miRs/miRNAs) were also identified, that were found to exert important functions as oncogenes or tumor suppressors (Gao et al, 2018; Liu et al, 2017). Compared with miRNAs, the discovery of LUAD-associated lncRNAs are still at a preliminary stage, but it has been demonstrated that they serve diverse and complex roles in tumorigenesis (Klingenberg et al, 2018).…”

Transcriptome-wide analysis reveals insight into tumor suppressor functions of 1B3, a novel synthetic miR-193a-3p mimic

“… 6 Subsequently, the 3p arm of miR-193a (miR-193a-3p) was demonstrated as the biologically active entity in tumor cell-based assays. A large body of evidence in the literature clearly shows that miR-193a-3p is frequently downregulated in malignant cells, including hepatocellular carcinoma (HCC), 7 non-small cell lung cancer (NSCLC), 8 and triple negative breast cancer (TNBC). 9 Significant evidence points toward a strong tumor-suppressive function of miR-193a-3p.…”