The clinical utility of TORCH testing of pregnant women from northeastern Romanian population: Application of electrochemiluminiscence assay

Ursu, Ramona Gabriela; Costin, Diana; Iancu, Luminiţa Smaranda; Nemescu, Dragoş

doi:10.1109/ehb.2015.7391504

Cited by 3 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This means that the infections are at the late stage of infection. Otherwise, anti-TORCH-IgM detection might be difficult in the early stage of infection, and might lead to false negative and positive results [14,15].…”

Section: Introductionmentioning

confidence: 99%

Serological study of TORCH complex in pregnant women with an obstetric history in Mosul city, Iraq

Mahmood¹,

Kahya²

2021

Current Trends in Immunology

View full text Add to dashboard Cite

Abortion is considered as one of the major problems in women which can lead to termination of fertility. Most abortion cases are directly linked with TORCH complex or congenital infections including bacterial, viral, and protozoal infections. Here, we collected 109 serum samples from pregnant women (study group) and 64 nonpregnant women (control group). The samples were directly examined for the presence of anti-TORCH-IgM and anti-TORCH-IgG antibodies against TORCH complex using enzyme-linked immunosorbent assay (ELISA) technique. Out of 109 pregnant in the study group, 51 cases (46.8%) were seropositive for IgG antibodies and 33 cases (30.25%) were seropositive for IgM antibodies. Non-pregnant had lower rates of TORCH complex seropositive. The majority of seropositive cases in the study group were between ages of 24-29 years. The highest rates of IgG seropositive for Toxoplasma were found in rural areas. The IgG seroprevalence rate of TORCH was highest in second trimester of pregnancy while the maximum rate of abortion was found to be 64% in the first trimester of pregnancy.

show abstract

Section: Introductionmentioning

confidence: 99%

Serological study of TORCH complex in pregnant women with an obstetric history in Mosul city, Iraq

Mahmood¹,

Kahya²

2021

Current Trends in Immunology

View full text Add to dashboard Cite

show abstract

“…TORCH screening to the pregnant women is helpful to provide critical clinical information for antenatal care (Li et al 2006). Different methods, for example, ELISA (enzyme linked immunosorbent assay), CLIA (chemiluminescence), ECLIA (electrochemiluminiscence) based on IgG or IgM have been developed for TORCH screening (Ursu et al 2015). Automated instruments are developed to implement screening for RUB or CMV infection in pregnancy to achieve convenient, rapid and reliable diagnosis with significantly reduced detection time (Van et al 2014;Revello et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Different methods, for example, ELISA (enzyme linked immunosorbent assay), CLIA (chemiluminescence), ECLIA (electrochemiluminiscence) based on IgG or IgM have been developed for TORCH screening (Ursu et al 2015). Automated instruments are developed to implement screening for RUB or CMV infection in pregnancy to achieve convenient, rapid and reliable diagnosis with significantly reduced detection time (Van et al 2014;Revello et al 2012). A rapid and low-cost TORCH screening method based on microarrays possessing comparable sensitivity and specificity with ELISA has been developed (Yang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

A bead-based microfluidic system for joint detection in TORCH screening at point-of-care testing

Qiu

Zhang

et al. 2017

Microsyst Technol

View full text Add to dashboard Cite

A concise bead-based microfluidic system has been developed for joint detection in TORCH screening at point-of-care testing. Assisted by five functionalized polycarbonate single beads, totally five critical infections including TOX, RUB, CMV, HSV-1 and HSV-2 can be simultaneously detected with the microfluidic chip based on chemiluminescence. The microfluidic chip consists of multiple reaction chambers respectively for different reaction steps of the sandwiched chemiluminescence immunoassay, and each reaction chamber is divided into five interconnected independent cells to respectively hold five single beads in different position. With a guiding path, each single bead with a ferrous core can be easily transported between each two adjacent reaction chambers through magnetic control to consecutively react with the serum sample or other different reagents. Meanwhile, with magnetic control, each single bead within the reaction chamber can be moved back and forth to improve the detection sensitivity with enhanced mixing. In one time test, joint detection with five infections to one serum sample can be simultaneously implemented by introducing synchronous manipulation to five single beads. Chemiluminescence signal from five single beads is collected by a sensitive CCD camera in parallel. Qualitative detection is achieved by comparing the detection signal amplitude with the corresponding cutoff value for each infection. Experimental results show that accurate, simple, convenient and efficient TORCH screening can be achieved with the easyto-operate and low-cost microfluidic system at resourcepoor settings.

show abstract

TORCH screening used appropriately in China?─three years results from a teaching hospital in northwest China

Wang

Yan²,

Ruan

et al. 2019

BMC Pregnancy Childbirth

View full text Add to dashboard Cite

ObjectivesTORCH infections caused by Toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV) and herpes simplex virus 1,2 (HSV-1,2) are associated with congenital anomalies. The study aimed to analyze the characteristics of TORCH screening in reproductive age women.MethodsA total of 18,104 women (2015–2017) from a teaching hospital in Xi’an, China, were enrolled in the study. The characteristics of TORCH screening, i.e., the application of TORCH test, the seroprevalence, the impact of age, periods of gestation and woman with bad obstetric history (BOH) on the serological data were investigated.ResultsIn the study, 319 women (1.76%) performed dynamic TORCH test. 51.66, 20.44 and 3.83% of the population did the test in the pre-gestation period, the first and third trimester, respectively. Quite a few pre-gestation women (29.74%) ignored screening of IgG antibodies. The overall IgG/IgM seropositvity of TOX, RV, CMV, HSV-1 and HSV-2 was 4.35%/0.35, 90%/0.63, 96.79%/0.97, 81.11%/0.14 and 6.1%/0.19%, respectively. The age-specific distributions and periods of gestation had no significant effect on the seroprevalence of TORCH agents, p>0.05. However, BOH was significantly associated with higher seropositvity of IgM (RV, CMV, HSV-1 and HSV-2) and IgG (CMV and HSV-1) antibodies, p < 0.05.ConclusionIn Xi’an region, more attentions should be paid to TOX, CMV, HSV-2 and the women with BOH for TORCH screening. Meanwhile, a greater emphasis needs to be placed on TORCH test used inappropriately in China.

show abstract

The clinical utility of TORCH testing of pregnant women from northeastern Romanian population: Application of electrochemiluminiscence assay

Cited by 3 publications

References 11 publications

Serological study of TORCH complex in pregnant women with an obstetric history in Mosul city, Iraq

Serological study of TORCH complex in pregnant women with an obstetric history in Mosul city, Iraq

A bead-based microfluidic system for joint detection in TORCH screening at point-of-care testing

TORCH screening used appropriately in China?─three years results from a teaching hospital in northwest China

Contact Info

Product

Resources

About