The clinical usefulness of the intraoperative detection of sentinel lymph node metastases by a rapid RT‐PCR system in patients with gastric cancer

Yanagita, Shigehiro; Uenosono, Yoshikazu; Arigami, Takaaki; Matsushita, Daisuke; Okubo, Kan; Kijima, Takashi; Arima, Hideo; Hirata, Munetsugu; Haraguchi, Naoto; Hagihara, Takahiko; Nishizono, Yuka; Ishigami, Sumiya; Natsugoe, Shoji

doi:10.1002/cncr.29740

Cited by 9 publications

(8 citation statements)

References 23 publications

(31 reference statements)

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“…We reported that SN were detected in 78 (99%) out of 79 patients with cT1N0 tumors by 99mTc-radiolabeled tin colloid methods, with sensitivity and accuracy rates of 100% [20]. We also demonstrated the clinical usefulness of the rapid RT-PCR system to detect sentinel metastasis in gastric cancer [22].…”

Section: Introductionmentioning

confidence: 72%

Quantitative assessment of fluorescence intensity of ICG in sentinel nodes in early gastric cancer

et al. 2018

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Section: Introductionmentioning

confidence: 72%

Quantitative assessment of fluorescence intensity of ICG in sentinel nodes in early gastric cancer

et al. 2018

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“…This study was planned as a preliminary study aimed at investigating methods for LN metastasis detection, and all samples were analyzed using a Light Cycler® 2.0 system. A previous study reported that the SLN concept may be applied to patients with early gastric cancer via RT-PCR using a Light Cycler system [10, 16, 19]. Ji et al [20] reported that CK19 mRNA RT-PCR assay using the Light Cycler system enables the detection of disseminated tumor cells in LN in EC.…”

Section: Discussionmentioning

confidence: 99%

“…However, this process is disadvantageous because of the long time taken for diagnosis, the shortage of expert pathologists, and the diagnostic difficulty of micrometastasis (MM) and isolated tumor cells (ITC). The intraoperative precise diagnosis based on reverse transcriptase polymerase chain reaction (RT-PCR) has clinical usefulness in gastric and breast cancers [6-10]. However, reports showing that the reverse transcription PCR (RT-PCR) method has clinical usefulness for LN metastasis in EC patients are few.…”

Section: Introductionmentioning

confidence: 99%

Quantitative RT-PCR Assay for Detecting Lymph Node Metastasis in Endometrial Cancer: A Preliminary Study

et al. 2018

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Objective: The detection accuracy of sentinel lymph node (SLN) metastasis by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) for endometrial cancer (EC) remains unclear and was assessed in this preliminary study. Methods: We studied primary cancer tissues and pelvic lymph nodes (PLN) from 105 patients with EC. qRT-PCR assay was performed to determine the copy numbers of CK19 mRNA in EC tissues, and negative and positive LN samples. Further, qRT-PCR results were compared with pathological findings. Results: CK19 mRNA expression was detected in 98% (104/106) of the tumors, with a median copy number of 3.0 × 10⁵/μL. Twelve LN were diagnosed as positive by pathological examination. The median copy number of CK19 mRNA for positive and negative LN was 8.1 × 10⁴/μL and 90.4/µL, respectively. CK19 mRNA expression was higher in pathologically positive LN than in pathologically negative LN (p < 0.01); the pathological and qRT-PCR findings showed no discrepancy. When the cutoff value was set at 4,500 copies/µL, qRT-PCR assay using CK19 mRNA exhibited high sensitivity and specificity. Conclusions: Our results demonstrated that qRT-PCR assay, using CK19 mRNA, exhibits a high accuracy for detecting LN metastasis in EC and represents a useful alternative to conventional pathological diagnosis of EC.

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“…Samples were analyzed using a prototype kit run on the Cepheid SmartCycler system (Cepheid, Sunnyvale, CA). This system has been shown to have clinical utility to finalize reverse transcription of the complementary DNA (cDNA) and amplification of the cDNA in one step within approximately 40 minutes, as previously described [ 25 ]. Moreover, this kit was designed to detect mRNA expressions of carcinoembryonic antigen (CEA), cytokeratin 19 (CK 19), and porphobilinogen deaminase as an internal control.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, this kit was designed to detect mRNA expressions of carcinoembryonic antigen (CEA), cytokeratin 19 (CK 19), and porphobilinogen deaminase as an internal control. The cutoff values of the threshold cycle (Ct) in CEA and CK 19 were set at 38 Ct and 37 Ct, respectively, as previously described [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Clinical application and outcomes of sentinel node navigation surgery in patients with early gastric cancer

et al. 2017

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Sentinel node navigation surgery (SNNS) has been recognized as a minimally invasive tool for individualized lymphadenectomy in patients with early gastric cancer (EGC). The aim of this study was to compare clinicopathological factors, adverse events, and clinical outcomes between sentinel node mapping (SNM) and SN dissection (SND) groups and assess the clinical utility of SNNS in patients with EGC. The clinical data of 157 patients with EGC, diagnosed as clinical T1N0M0 with tumors ≤ 40 mm, undergoing SNNS between March 2004 and April 2016 were retrospectively reviewed. Twenty-seven patients were excluded from the analysis. In the remaining 130 patients, 59 and 71 patients underwent standard lymphadenectomy for SNM and SND, respectively. The sentinel node detection rate in the SNM and SND groups was 98.3% (58/59) and 100% (71/71), respectively. Two (3.5%), 15 (25.9%), and 41 (70.7%) patients having sentinel nodes underwent total gastrectomy, proximal gastrectomy (PG), and distal gastrectomy (DG), respectively, in the SNM group. One (1.4%), 5 (7.0%), 10 (14.1%), 39 (54.9%), and 16 (22.5%) patients underwent PG, DG, segmental gastrectomy, local resection, and endoscopic submucosal dissection, respectively, in the SND group. There was no significant difference in postoperative complications between the SNM and SND groups (P = 0.781). Survival did not differ between the both groups (P = 0.856). The present results suggest that personalized surgery with SND provides technical safety and curability related with a favorable survival outcome in patients with EGC.

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The clinical usefulness of the intraoperative detection of sentinel lymph node metastases by a rapid RT‐PCR system in patients with gastric cancer

Cited by 9 publications

References 23 publications

Quantitative assessment of fluorescence intensity of ICG in sentinel nodes in early gastric cancer

Quantitative assessment of fluorescence intensity of ICG in sentinel nodes in early gastric cancer

Quantitative RT-PCR Assay for Detecting Lymph Node Metastasis in Endometrial Cancer: A Preliminary Study

Clinical application and outcomes of sentinel node navigation surgery in patients with early gastric cancer

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