The clinical significance of the &lt;em&gt;Ezrin&lt;/em&gt; gene and circulating tumor cells in osteosarcoma

Zhong, Guang‐Xian; Feng, Shao-Dan; Shen, Rongkai; Wu, Zhaoyang; Chen, Fei; Zhu, Xiaoyang

doi:10.2147/ott.s125589

Cited by 24 publications

(25 citation statements)

References 37 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that CTCs, particularly M-CTCs, may play a crucial role in the metastasis of osteosarcoma. The results of thisstudy are consistent with those reported by Zhong et al; 17 the CTC counts correlated significantly with the Enneking stage ( P <0.001), and the ratio of M-CTCs correlated with the distant metastases ( P <0.001).…”

Section: Discussionsupporting

confidence: 93%

Significance of circulating tumor cells in osteosarcoma patients treated by neoadjuvant chemotherapy and surgery

Wu¹,

Tan²,

Xiong³

et al. 2018

CMAR

View full text Add to dashboard Cite

PurposeBy examining and identifying circulating tumor cell (CTC) counts and subtypes of peripheral blood in osteosarcoma patients, we evaluated the relationship between CTCs and characteristics of osteosarcoma patients, as well as CTC changes after neoadjuvant chemotherapy and surgery.MethodsCanPatrol™ CTC technology was used to detect CTCs in peripheral blood before and after treatment in 32 osteosarcoma patients. Peripheral blood samples from 10 healthy volunteers were included as controls and examined for the presence of CTCs.ResultsOf the 32 osteosarcoma patients, CTCs were detected in 30 patients before treatment, and the average CTC count was 14.06±9.08. No CTCs were detected in the 10 healthy volunteers. The detected CTCs were divided into epithelial CTCs, mesenchymal CTCs (M-CTCs), and biophenotypic epithelial/mesenchymal CTCs. The average number of pretreatment CTCs was higher in stage III patients than in stage IIB patients (P=0.012). Twenty-eight patients were screened for changes in CTC count at 1 week after neoadjuvant chemotherapy and at 4 weeks after surgery. We divided these 28 patients into two groups according to the changes in the percentage of M-CTCs before and after treatment, and the results showed that the disease-free survival (DFS) was significantly shorter in the M-CTC percentage-increased group than in the M-CTC percentage-decreased or no-change group (P=0.032). Five patients with stage II osteosarcoma were examined for CTCs at the appearance of lung metastases, and the total number of CTCs was found to be higher at the appearance of lung metastases than before treatment in these patients.ConclusionThe rate of presence of CTCs in the peripheral blood of osteosarcoma patients is high, and patients with an increased percentage of M-CTCs after treatment have a shorter DFS. The dynamic monitoring of changes in CTC counts after treatment has clinical significance for the timely detection of recurrence or metastasis.

show abstract

Section: Discussionsupporting

confidence: 93%

Significance of circulating tumor cells in osteosarcoma patients treated by neoadjuvant chemotherapy and surgery

Wu¹,

Tan²,

Xiong³

et al. 2018

CMAR

View full text Add to dashboard Cite

show abstract

“…miR-183-5p is critical in various types of tumor, including hepatocellular carcinoma, osteosarcoma and breast cancer ( 14 ). Ezrin belongs to the ezrin/radixin/moesin family and is a membrane cytoskeletal linker protein functioning in the metastasis of several types of human cancer, including breast cancer, colorectal carcinoma, gastric cancer and serous ovarian carcinoma ( 15 ). In addition, the upregulation of miR-183 represses cell migration and invasion via targeting Ezrin in lung and breast cancer ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Upregulation of miR-183-5p is responsible for the promotion of apoptosis and inhibition of the epithelial-mesenchymal transition, proliferation, invasion and migration of human endometrial cancer cells by downregulating Ezrin

et al. 2018

View full text Add to dashboard Cite

Endometrial cancer is a life-threatening malignancy that affects women all over the world, and it has an increasing incidence. MicroRNAs (miRNAs/miRs) have been reported to be involved in cellular activities in endometrial cancer. The present study aimed to examine the effects of miR-183-5p on the epithelial-mesenchymal transition (EMT), proliferation, invasion, migration and apoptosis of human endometrial cancer cells by targeting Ezrin. Primary endometrial cancer tissues and adjacent normal tissues were obtained for the investigation. The protein expression of Ezrin in tissues was detected by immunohistochemistry. The expression level of miR-183-5p and the mRNA and protein expression levels of Ezrin and EMT-associated genes were determined by reverse transcription-quantitative polymerase chain reaction and western blot analyses. Endometrial cancer cells were treated with miR-183-5p inhibitors, small interfering RNA targeting Ezrin or miR-183-5p inhibitors. Cell proliferation, cell cycle, apoptosis, migration and invasion were then evaluated using an MTT assay, flow cytometry, scratch test and Transwell assay, respectively. Compared with normal adjacent tissues, the expression of miR-183-5p was decreased in endometrial cancer tissues, and the expression of Ezrin was significantly increased in endometrial cancer tissues. The protein expression of Ezrin was correlated with the severity and poor prognosis of endometrial cancer. Notably, the target prediction program and the luciferase reporter gene assay confirmed that miR-183-5p targeted and negatively regulated the expression of Ezrin. In vivo experiments revealed that the increased expression of miR-183-5p and decreased expression of Ezrin inhibited EMT, cell proliferation, migration and invasion, but promoted cell apoptosis in Ishikawa cells. These results suggested that the upregulated expression of miR-183-5p promoted apoptosis and suppressed the EMT, proliferation, invasion and migration of human endometrial cancer cells by downregulating Ezrin.

show abstract

“…[38,39,40] Ezrin, is a member of the protein family that normally binds the cellular skeleton ERM (ezrin, radixin, and moesin). [41] Ezrin occurs mainly in the inactive form in the cytoplasm, and it transforms to a special active form after the activation with treonin and tirosin phosphorylation. The main biologic function of ezrin is to bind the transmembrane proteins to actin cell skeleton.…”

Section: Treatment Of Osteosarcomamentioning

confidence: 99%

“…Therefore, the ezrin expression plays a significant role in the lung metastasis of the OS cells. [41,42] Receptor Tyrosine Kinases: Ewing sarcoma is a malignant tumor and a member of the round-cell tumors group that is commonly detected between the age of 5 and 25 years and is located in the diaphysis region of the long bones. Ewing sarcoma is the second most commonly detected sarcoma after OS and is detected in the out-of-bone soft tissue location.…”

Section: Treatment Of Osteosarcomamentioning

confidence: 99%

Genetics of Osteosarcomas

ÖZDENOĞLU¹

2020

TJ Oncol

View full text Add to dashboard Cite

Osteosarcoma (OS) is the most common bone cancer in children and young adults. Most cases are high grade and aggressive. Studies on the OS genetics have become important in recent years. The reason for the small number of studies on OSs is because the tumor is rarely detected. OSs mostly develop in the femur, tibia, and humerus. Overt metastasis may be detected in 20%-25% of cases. The first genetic studies of OSs were the studies conducted at the cytogenetic level. They showed that OSs had aneuploidic characteristics. Because studies for identifying the gene expression levels used the cytogenetic and molecular analyses, substantial data were available on OSs. In addition, OSs have been found to have a wide miRNA spectrum. The results obtained particularly from the circRNA and miRNA studies suggested that these molecules might be used as biomarkers in the identification of the drug resistance, which reveals the importance of genetic changes in the follow-up of diagnosis and treatment. The aim of the present review was to collect the cytogenetic and molecular studies of OSs and to summarize the genetic data of OSs.

show abstract

The clinical significance of the <em>Ezrin</em> gene and circulating tumor cells in osteosarcoma

Cited by 24 publications

References 37 publications

Significance of circulating tumor cells in osteosarcoma patients treated by neoadjuvant chemotherapy and surgery

Significance of circulating tumor cells in osteosarcoma patients treated by neoadjuvant chemotherapy and surgery

Upregulation of miR-183-5p is responsible for the promotion of apoptosis and inhibition of the epithelial-mesenchymal transition, proliferation, invasion and migration of human endometrial cancer cells by downregulating Ezrin

Genetics of Osteosarcomas

Contact Info

Product

Resources

About