The clinical significance of conversion of complement-dependent cytotoxic T cell crossmatch test after renal transplantation

Abstract

The purpose of this study was to investigate the clinical relevance of conversion of post-transplant T cell crossmatch between kidney donor and recipient. This study comprises 892 cadaveric renal transplantations performed on 874 adult patients between August 1991 and December 1997. Recipient selection was based on a negative complement-dependent cytotoxic T cell crossmatch test with current ( 5 2 months old) serum. For this study, on day 0 and day 14 after transplantation, serum samples were collected for later crossmatching. On day 14 after transplantation, the crossmatch had converted to positive in 76 transplantations (8.5 Yo).Acute rejection occurred in 50 YO of the converters and 22 % of the nonconverters ( P < 0.005), and graft survival was significantly poorer ( P < 0.025), being 85 vs 94 YO at 1 and 68 vs 83 YO at 5 years, respectively. In patients with delayed graft function, l-year graft survival was 77 Yo in the converters and 91 YO in the non-converters ( P < 0.05). Conversion of T cell crossmatch, especially in connection with delayed graft function, identifies a subgroup of patients at high risk of severe rejection and poor graft survival.

Collecting high‐quality pancreatic tissue for experimental study from organ donors with signs of β‐cell autoimmunity

“…The process began with the identification of a possible organ donor in a local hospital in Finland (Figure 1). The National Transplantation Center was always contacted because they coordinate every organ retrieval in Finland 15, 16. Blood samples were taken from the potential donor for routine analysis of ABO blood group and HLA type (The Finnish Red Cross Blood Service, Clinical Laboratory, Helsinki) and for viral analysis (human immunodeficiency virus, cytomegalovirus and hepatitis B and C) carried out at the Helsinki University Hospital Laboratory, Department of Virology.…”