The clinical significance of apolipoprotein L1 in head and neck squamous cell carcinoma

Abstract

Approximately 500,000 new head and neck squamous cell carcinoma (HNSCC) cases are detected every year around the world, and its incidence ranks sixth among all cancer types globally. Among these cases, oral squamous cell carcinoma (OSCC) and laryngeal squamous cell carcinoma (LSCC) are HNSCC subtypes with high incidence rates, especially in China. The present study examines the association between the apolipoprotein L1 (APOL1) mRNA and protein expression and clinical parameters in HNSCC. The two most common types (oral and larynx) of HNSCC were selected for subgroup analyses. Immunohistochemistry (IHC) was used to detect APOL1 protein expression levels in HNSCC clinical specimens. It was demonstrated that APOL1 protein expression in 221 cases of HNSCC was higher compared with that in normal tissues. Consistent upregulation of APOL1 protein was also found in subgroups of OSCC and LSCC. Through mining the ArrayExpress, The Cancer Genome Atlas and the Gene Expression Omnibus databases, microarrays and RNA sequencing data for HNSCC were retrieved, which were used to analyze APOL1 mRNA expression levels. The results showed that APOL1 expression was higher in both OSCC and LSCC subtypes, as well as in HNSCC, compared with that in non-cancerous squamous epithelium. The summary receiver operating characteristic analysis showed that APOL1 had potential as a diagnostic biomarker for HNSCC, OSCC and LSCC. Thus, upregulation of APOL1 may contribute to the tumorigenesis of HNSCC.

Bioinformatic analysis identifying Endoplasmic Reticulum Stress-Related genes as a new diagnostic and prognostic indicator in clear cell Renal Cell Carcinoma

“…Consistently, the expression of APOL1, COL5A3, TNFSF13B and CDH5 in tumor tissues was signi cantly higher than adjacent normal tissues. Notably, the result of RT-qPCR and IHC was consistent with our previous results (Figure 13,14).…”

“…APOL1 belongs to the APOL gene family, a minor component of high-density lipoprotein (HDL), which is associated with a variety of cancers. In previous studies, APOL1 was thought to be highly elevated in various cancers (13)(14)(15)(16)). Jiewei Lin et al found that APOL1 was signi cantly up-regulated in pancreatic cancer and was associated with advanced pathological stage, lymph node metastasis and distant metastasis, which could be used as both a prognostic biomarker and a new diagnostic target for pancreatic cancer patients (17).…”

Clinical assessment and molecular mechanism of the upregulation of Toll-like receptor 2 (TLR2) in myocardial infarction

“…After TLR2 expression was extracted from each dataset or study, the standardized mean difference (SMD) was calculated by constructing a forest map [ 18 – 21 ]. The I 2 statistics was used to assess the heterogeneity of the involved data as previously reported [ 22 , 23 ]. A summary receiver operating characteristic curve (sROC) was generated to check the prospective diagnostic value of TLR2 for MI.…”

WDHD1 is over‐expressed in nasopharyngeal carcinoma and may control the expression of ITGAV

“…The primary antibody (rabbit polyclonal antibody of WDHD1, ab224221, Abcam (Cambridge, MA, USA); rabbit monoclonal antibody of ITGAV, ab179475, Abcam) was diluted (Zhong Shan Golden Bridge Biotechnology, Beijing, China) at a ratio of 1 : 100 and then incubated at 37 °C for 70 min. The rest of the steps were performed according to a previously reported procedure [ 13 ]. Staining was assessed under a light microscope, and we used two different methods to assess the IHC results of WDHD1 and ITGAV.…”