The Clinical, Serological and Molecular Diagnosis of Emerging Dengue Infection at a Tertiary Care Institute in Southern, India

Abstract

Introduction: Dengue is an acute viral infection which presents as uneventful pyrexia to a fatal complication. This infection is increasingly being recognized as the world's major emerging tropical disease and an important public health problem.This article highlights the clinical manifestations of Dengue virus infection and the various molecular tests that were used for its laboratory diagnosis.Methods: Serum samples from 713 suspected cases of Dengue were collected between August and December 2007. The clinical profiles of 123 hospitalized patients were analyzed. Serology, RT-PCR, virus isolation and sequencing were done. Results:The most common clinical symptoms were fever, thrombocytopenia, rash and elevated liver enzymes. The demonstration of the Dengue RNA in 5.16% samples, the detection of Dengue specific IgM antibodies in 18% samples and the isolation of the DENV-4 and the DENV-3 viruses from the clinical samples confirmed this Dengue outbreak. A co -infection with Chikungunya was observed in 2.06% of the cases. The phylogenetic analysis revealed that the Indian Dengue-4 isolates from this outbreak belonged to the genotype I. This study clearly indicated the sudden dominance of DENV-4 in an Indian Dengue outbreak. Conclusion:The surveillance of the Dengue viruses needs to be closely monitored for the emergence of newer serotype(s) in hitherto unknown areas.

Dengue Fever; The Clinical Pattern and Mortality in Epidemic and Post Epidemic Years in Swat

“…Similar age pattern was reported from India, Myanmar, Karachi and Lahore. 19,20,21,22,23 Unlike the observation of Garg et al, the least number of children were affected by Dengue fever in our study (1%). 18 For seasonal variation, month wise data was analyzed and it was observed that majority of cases were reported during month of September, which is a post monsoon period in the valley.…”

Dengue in Children: Prolife of Hematological and Biochemical parameters.

“…In india ,dengue was first reported in 1946, DENV-1 was first reported in 1956 at vellore,DENV -2 in 1956, DENV -3 in 1965 and DENV -4 in 1964 [10] [11] . Due to changing climate, urbanization poor living conditions and inadequate waste management, vector borne disease like dengue fever are becoming more common.…”

Dengue fever: atypical manifestation

“…The organ failure occurs, secondary to multiorgan dysfunction syndrome. The other unusual manifestations include Transient paresis of limbs, spasticity, cranial nerve palsies 3,6,7 and convulsions. Fatal cases with encephalitic manifestations have been reported in Indonesia, Malaysia, Myanmar, India and Puerto Rico.…”