The clinical efficacy of primary culture of human fibroblasts in gingival augmentation procedures—A preliminary report

Dominiak, Marzena; Łysiak–Drwal, Katarzyna; Saczko, Jolanta; Kunert‐Keil, Christiane; Gedrange, Tomasz

doi:10.1016/j.aanat.2012.03.011

Cited by 19 publications

(8 citation statements)

References 26 publications

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“…No specimens revealed an inflammatory response. A complete clinical healing was observed after 2 weeks in 8 out of 10 patients [25]. This is in agreement with further studies using human gingival fibroblasts on a biodegradable collagen scaffold [26,27].…”

Section: Surgical Therapy Of Gingival Recessionsupporting

confidence: 78%

New Perspectives in the Diagnostic of Gingival Recession

Dominiak¹,

Gedrange²

2014

Adv Clin Exp Med

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Gingival recession (GR) is a common clinical situation observed in patient populations regardless of their age and ethnicity. It has been estimated that over 60% of the human population has gingival recession. It is the final effect of the interaction of multiple etiological factors. Identification and definition of the range of influence is often not possible, with the result that new methods for testing and elimination of potential etiological factors are still being sought. The aim of this study is to present the etiopathogenesis of gingival recessions with regard to the analysis of morphological and functional factors. For the assessment of the bone factors, we will describe the new cephalometric method for measuring sagital width of the bone in the central incisors area, places when GR are most commonly observed. Also, a review will be presented of modern methods of treatment; in particular classes recessions; usage substitute of autogenous tissue will be emphasized -collagen matrix, and primary culture fibroblasts on collagen net (Adv Clin Exp Med 2014, 23, 6, 857-863).

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Section: Surgical Therapy Of Gingival Recessionsupporting

confidence: 78%

New Perspectives in the Diagnostic of Gingival Recession

Dominiak¹,

Gedrange²

2014

Adv Clin Exp Med

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“…Nunes describes a rare occurrence of bilateral fusion of mandibular second molars with supernumerary teeth and their endodontic therapy (14). The positive infl uence of PRP on post-operational wound healing in the oral cavity is described by many authors (1,2,5,11,12).…”

Section: Discussionmentioning

confidence: 99%

Fusion of the 2nd maxillary molar with the impacted 3rd molar

Strecha¹,

Jurkovič²,

Siebert³

2012

BLL

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Subject matter:The dentist has to deal with complicated cases of fused molars, which are rather rare and morphologically very varied. A wrong or incomplete diagnosis can considerably complicate a planned therapy. Case report: The authors describe a case of apical periodontal complication of fused teeth that had to be removed surgically. The upper 2nd molar fused with the impacted 3rd molar and was diagnosed for extraction. Conclusion: Even a careful diagnostic procedure and X-ray image sometimes may not indicate the exact location and mutual position of the fused teeth. The authors make us aware of the possible occurrence of fused roots, and the necessity to inform the patient ahead of time about the course of endodontic or surgical interventions, possible complications and their removal. They describe the positive infl uence of PRP (platelet rich plasma) in wound healing. In order to establish the exact indication and therapy, they emphasize the importance of using CT imaging diagnostics or a 3D-CT examination (Fig. 7, Ref. 15). Full Text in PDF www.elis.sk.

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“…In literature there are known studies on comparisons of physical and chemical properties of type I collagen from bird feet, bovine skin, porcine skin, frog skin and shark skin [8]. The study of Song et al [3] shows that a novel form of acid-soluble collagen was extracted from jellyfish.…”

Section: Discussionmentioning

confidence: 99%

“…Their amino acid composition, peptide constitution, glycosaminoglycan content and thermal behavior are significantly different from land animals. On the basis on Lin et al [8] study, it has been established that bird feet and porcine skin collagen should be used as a suitable material utility because of their better biostability. In the course of other research, it was established that fish collagen and its peptides have stimulatory influence on some cellular and tissue specific processes, e.g.…”

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confidence: 99%

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An Estimation of the Biological Properties of Fish Collagen in an Experimental In Vitro Study

Kuzan¹,

Smulczyńska-Demel²,

Chwiłkowska³

et al. 2015

Adv Clin Exp Med

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Background. The principal sources of medical collagen are pork, calf skin and bone. There are now more studies on a much safer, alternative source of active collagen, mainly from aquatic life. Active collagen and its peptides FCP (fish collagen peptides) have already been extracted from the skin of salmon, cobia, hoki, tilapia, zebrafish, ling, shark, silver carp and also jellyfish. Objectives. The aim of the study is to evaluate the effect of fish collagen on human fibroblasts from gingiva. The cytotoxicity of the new formulation and induction of endogenous collagen was estimated by means of the collagen derived from fish skin. Material and Methods. Fish collagen was extracted from the skin of silver carp at 16 degrees Celsius. To compare the biocompatibility and endogenous collagen production Geistlich Bio-Gide ® membrane was ordered in Geistlich Biomaterials (Geistich AG, Wolhusen, Switzerland). The culture of human fibroblasts was performed acc. to Saczko et al. The fibroblasts were treated 96 hours with 1.0%, 0.5% and 0.1% experimental collagen formulation to induce endogenous collagen production. The Sircol collagen assay was done to measure amount of collagen. Cell viability was assessed by measuring mitochondrial activity in MTT assay after 24 h followed by 24 h of incubation with experimental collagen formulation. Qualitative analysis was performed by immunocytochemically staining of collagen type I and III. Results. Preparations of fish collagen are not cytotoxic at concentrations below 1%. Cells cultured in the presence of this product are characterized by a large number of endogenous collagen, which is comparable to the control. In case of porcine collagen membrane was noticed decreased to 83% production of endogenous collagen and reduction of cell viability to 69%. Conclusions. Our study showed that experimental fish collagen is an innovative product which may induce expression of endogenous collagen in fibroblasts (Adv Clin Exp Med 2015, 24, 3, 385-392).

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The clinical efficacy of primary culture of human fibroblasts in gingival augmentation procedures—A preliminary report

Cited by 19 publications

References 26 publications

New Perspectives in the Diagnostic of Gingival Recession

New Perspectives in the Diagnostic of Gingival Recession

Fusion of the 2nd maxillary molar with the impacted 3rd molar

An Estimation of the Biological Properties of Fish Collagen in an Experimental In Vitro Study

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