The price of stingless bee (Heterotrigona itama) honey is almost twice higher than the one produced by a non-stingless bee (Apis mellifera) due to its limited production and high content of polyphenol and flavonoids compounds. However, in the market, the authentication of stingless bees and non-stingless bees honey becomes difficult due to the similarity in the color of honey and its sugar content. In this research, we propose a simple analytical approach by combining ultraviolet (UV) spectroscopy and hierarchical cluster analysis (HCA) for discrimination between stingless and non-stingless bee honey. Fifty samples of monofloral stingless bee (Heterotrigona itama) honey and non-stingless bee (Apis mellifera) honey from Acacia mangium was used. The samples were diluted with a distilled water at a proportion of 1:30 (volume/volume). The spectral data were acquired in the range of 190-1100 nm using a benchtop UV-Vis spectrometer with fast scanning mode. The HCA was applied for selected preprocessed spectral data in the range of 230-400 nm. The result shows that HCA could be effective to discriminate between stingless and non-stingless bee honey. Shortly, it is expected to realize the simple and quick analytical method to authenticate stingless bee honey based on UV spectroscopy and the HCA method.