The Class III peroxidase gene <i>OsPrx30</i>, transcriptionally modulated by the AT‐hook protein OsATH1, mediates rice bacterial blight‐induced ROS accumulation

Líu, Hao; Dong, Shuangyu; Li, Ming; Gu, Fengwei; Yang, Gangyi; Guo, Tao; Chen, Zhiqiang; Wang, Jiafeng

doi:10.1111/jipb.13040

Cited by 40 publications

(46 citation statements)

References 54 publications

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“…The AT‐hook facilitates binding specificity to AT‐rich DNA sequences at nuclear matrix attachment regions (MARs), whereas DUF296 enables protein‐protein interactions. The AHL protein has diverse roles in the plant developmental process, including hypocotyl elongation, gibberellin biosynthesis, pollen nexine formation and flower development (Liu et al ., 2021a). AT‐rich sequences are generally found in flanking regions such as the transcriptional units or regions close to cis ‐regulatory elements.…”

Section: Discussionmentioning

confidence: 99%

Single‐cell RNA‐seq describes the transcriptome landscape and identifies critical transcription factors in the leaf blade of the allotetraploid peanut (Arachis hypogaea L.)

Líu

et al. 2021

Plant Biotechnology Journal

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Summary Single‐cell RNA‐seq (scRNA‐seq) has been highlighted as a powerful tool for the description of human cell transcriptome, but the technology has not been broadly applied in plant cells. Herein, we describe the successful development of a robust protoplast cell isolation system in the peanut leaf. A total of 6,815 single cells were divided into eight cell clusters based on reported marker genes by applying scRNA‐seq. Further, a pseudo‐time analysis was used to describe the developmental trajectory and interaction network of transcription factors (TFs) of distinct cell types during leaf growth. The trajectory enabled re‐investigation of the primordium‐driven development processes of the mesophyll and epidermis. These results suggest that palisade cells likely differentiate into spongy cells, while the epidermal cells originated earlier than the primordium. Subsequently, the developed method integrated multiple technologies to efficiently validate the scRNA‐seq result in a homogenous cell population. The expression levels of several TFs were strongly correlated with epidermal ontogeny in accordance with obtained scRNA‐seq values. Additionally, peanut AHL23 (AT‐HOOK MOTIF NUCLEAR LOCALIZED PROTEIN 23), which is localized in nucleus, promoted leaf growth when ectopically expressed in Arabidopsis by modulating the phytohormone pathway. Together, our study displays that application of scRNA‐seq can provide new hypotheses regarding cell differentiation in the leaf blade of Arachis hypogaea. We believe that this approach will enable significant advances in the functional study of leaf blade cells in the allotetraploid peanut and other plant species.

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Section: Discussionmentioning

confidence: 99%

Single‐cell RNA‐seq describes the transcriptome landscape and identifies critical transcription factors in the leaf blade of the allotetraploid peanut (Arachis hypogaea L.)

Líu

et al. 2021

Plant Biotechnology Journal

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“…Several TFs bind to PRX promoters to activate or repress their expression and modulate POD activity, which is involved in lignified tissue formation, seed longevity, disease resistance and abiotic stress tolerance in plants (Huang et al, 2013;Liu et al, 2015Liu et al, , 2021Cosio et al, 2017;Renard et al, 2020). However, the TFs involved in regulating PRX-mediated H 2 O 2 scavenging under multiple abiotic stress conditions are still poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…Subcellular localization markers fused to mCherry include the nuclear marker NLS-mCherry, the endoplasmic reticulum (ER) marker HDEL-mCherry, the Golgi marker Man49-mCherry, and the membrane marker PIP2A-mCherry (Nelson et al, 2007). Rice protoplasts were prepared as previously described (Liu et al, 2021). Protoplasts were resuspended in WI solution (4 mM MES, 0.5 M mannitol, 20 mM KCl, pH 5.8) for 16-22 h in the dark after transient transfection.…”

Section: Subcellular Localization Analysismentioning

confidence: 99%

The IbBBX24–IbTOE3–IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato

Zhang

Wang

et al. 2021

New Phytologist

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Soil salinity and drought limit sweet potato yield. Scavenging of reactive oxygen species (ROS) by peroxidases (PRXs) is essential during plant stress responses, but how PRX expression is regulated under abiotic stress is not well understood.Here, we report that the B-box (BBX) family transcription factor IbBBX24 activates the expression of the class III peroxidase gene IbPRX17 by binding to its promoter. Overexpression of IbBBX24 and IbPRX17 significantly improved the tolerance of sweet potato to salt and drought stresses, whereas reducing IbBBX24 expression increased their susceptibility. Under abiotic stress, IbBBX24-and IbPRX17-overexpression lines showed higher peroxidase activity and lower H 2 O 2 accumulation compared with the wild-type. RNA sequencing analysis revealed that IbBBX24 modulates the expression of genes encoding ROS scavenging enzymes, including PRXs.Moreover, interaction between IbBBX24 and the APETALA2 (AP2) protein IbTOE3 enhances the ability of IbBBX24 to activate IbPRX17 transcription. Overexpression of IbTOE3 improved the tolerance of tobacco plants to salt and drought stresses by scavenging ROS.Together, our findings elucidate the mechanism underlying the IbBBX24-IbTOE3-IbPRX17 module in response to abiotic stress in sweet potato and identify candidate genes for developing elite crop varieties with enhanced abiotic stress tolerance.

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“…Only very limited information is available about the regulation of secretory peroxidases. Their transcriptional control is mediated by AT-hook protein OsATH1 in rice ( Liu et al, 2021 ) or by AGAMOUS-LIKE15 (AGL15) in Arabidopsis ( Cosio et al, 2017 ). The mechanism of MAPK-dependent regulation of peroxidases is not resolved yet.…”

Section: Discussionmentioning

confidence: 99%

TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley

et al. 2021

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Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22.

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The Class III peroxidase gene OsPrx30, transcriptionally modulated by the AT‐hook protein OsATH1, mediates rice bacterial blight‐induced ROS accumulation

Cited by 40 publications

References 54 publications

Single‐cell RNA‐seq describes the transcriptome landscape and identifies critical transcription factors in the leaf blade of the allotetraploid peanut (Arachis hypogaea L.)

Single‐cell RNA‐seq describes the transcriptome landscape and identifies critical transcription factors in the leaf blade of the allotetraploid peanut (Arachis hypogaea L.)

The IbBBX24–IbTOE3–IbPRX17 module enhances abiotic stress tolerance by scavenging reactive oxygen species in sweet potato

TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley

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