The Cj0588 protein is a Campylobacter jejuni RNA methyltransferase

Sałamaszyńska-Guz, Agnieszka; Taciak, Bartłomiej; Kwiatek, Agnieszka; Klimuszko, D.

doi:10.1016/j.bbrc.2014.04.104

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…The authors attributed this motility defect to a defect in flagellar appearance when analyzed under transmission electron microscope. A similar reduction in motility was also observed in another 81-176 MTase mutant (cj0588) and exhibited 70% loss of motility in the mutant compared to wildtype (Sałamaszyńska-Guz et al, 2014).…”

Section: Discussionsupporting

confidence: 72%

“…Interestingly, MTases can also modify ribosomal RNA genes by post-transcriptional methylation and are most well-characterized in Escherichia coli (Baldridge and Contreras, 2014). Several studies have found that knocking out the MTase gene conferred an increased level of antibiotic resistance (LaMarre et al, 2011;Mikheil et al, 2012;Monshupanee et al, 2012;Sałamaszyńska-Guz et al, 2014). These findings, combined with our identification of these genome regions as hypomethylated, suggest that further studies are warranted to determine the physiologic rationale for the hypomethylation of these rRNA genes.…”

Section: Discussionmentioning

confidence: 78%

“…Several other studies have also linked the function of MTases with specific factors important for C. jejuni virulence such as motility, adherence, invasion, and colonization of the host (Kim et al, 2008;Anjum, 2013;Sałamaszyńska-Guz et al, 2014). However, these studies give a limited view of the functional impact these MTases have.…”

Section: Discussionmentioning

confidence: 99%

“…These assays can provide a closer look at examining which stages of colonization are affected by the mutagenesis. These assays have been tested in other C. jejuni MTase mutants and while the results have been mixed depending on the strain, MTase mutants were observed to either have no differences in adhesion and invasion (O'Loughlin et al, 2015), display hyper-adherence but decreased invasion ability (Kim et al, 2008), or severely reduced adhesion and/or invasion abilities compared to wildtype (Anjum, 2013;Sałamaszyńska-Guz et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have found that mutating the MTase genes in C. jejuni have functional consequences on a variety of phenotypes including motility, in vivo colonization, antibiotic resistance, and in vitro adhesion and invasion ability (Kim et al, 2008;Anjum, 2013;Sałamaszyńska-Guz et al, 2014). To simulate some of those phenotypic studies in our mutant, we first tested the growth ability of IA3902ΔRS00180.…”

Section: Effects Of the Mutation Of Cjsa_rs00180 On Growth Motility mentioning

confidence: 99%

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Mechanisms and roles of the LuxS system, methyl recycling, and DNA methylation on the physiology of Campylobacter jejuni

Mou

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Introduction iii Materials and Methods Results Discussion Acknowledgements Author Contributions Figures legends Tables CHAPTER 4. CHARACTERIZATION OF THE CJSA_RS00180 DNA METHYLTRANSFERASE IN CAMPYLOBACTER JEJUNI IA3902 Abstract Introduction Materials and Methods Results Discussion Figures legends Tables CHAPTER 5. General Conclusions General Discussion Recommendations for Future Research ix ACKNOWLEDGEMENTS This thesis was made possible by the collective efforts and support of colleagues, friends, and family. First, I would like to express my sincerest gratitude to my major professor, Dr. Paul Plummer, as he has been an invaluable mentor at every step of my graduate studies. I am thankful for his kindness, patience, expertise, and the time he has spent answering all of my questions, challenging me and instilling confidence in myself as a scientist, improving my writing, and helping me gain access to innumerable opportunities, all of which have helped me develop into a successful independent researcher. I am very grateful for the opportunity to work in his laboratory. I would also like to thank my program of study committee: Drs. Qijing Zhang, Michael Yaeger, Lisa Nolan, and Gwyn Beattie for their guidance, suggestions, and encouragement. I thank all the members of the Plummer lab and Zhang lab, whom I have had the privilege to work with, and have invested countless number of hours to help assist me with experiments and training, sharing their wisdom and expertise in all things Campylobacter, and have always been available for me to exchange thoughts and ideas with. My research successes would not have been possible without your support and guidance. I also thank the countless number of former and current employees at the Iowa State University College of Veterinary Medicine and at the Interdepartmental Microbiology program for their support throughout my graduate career. Lastly, I would like to sincerely thank my parents Dach and Sherry, my sister Connie, Alan, Marty, Brent, Anna, and all my friends for their unwavering love and invaluable support network. For without their continuous love and support, I would not be where I am today.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Effects Of the Mutation Of Cjsa_rs00180 On Growth Motility mentioning

confidence: 99%

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Mechanisms and roles of the LuxS system, methyl recycling, and DNA methylation on the physiology of Campylobacter jejuni

Mou

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Campylobacter

McCarthy¹

2017

Applied Genomics of Foodborne Pathogens

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The Forgotten Virulence Factor: The ‘non-conventional’ Hemolysin TlyA And Its Role in Helicobacter pylori Infection

Javadi

Katzenmeier

2016

Curr Microbiol

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Helicobacter pylori is a human-specific Gram-negative pathogenic bacterium which colonizes the gastric mucosal layer in the stomach causing diseases such as peptic ulcer, adenocarcinoma, and gastric lymphoma. It is estimated that approximately half of the world's population is infected with H. pylori making it the most intensively characterized microbial pathogen up to now. Hemolysis has been suggested to significantly contribute to colonization of the stomach and disease progression by H. pylori. In a number of earlier studies, TlyA was characterized as a putative pore-forming cytolysin. Although a few observations in the literature suggest a role for TlyA as significant virulence factor of H. pylori, the molecular and structural characterization of this protein is much curtailed at present. Given the intensive characterization of numerous H. pylori virulence factors over the past decade, surprisingly little information exists for the TlyA toxin and its significance for pathogenesis. This review provides a brief overview on microbial hemolysis and its role for pathogenesis and discusses recent research efforts aimed at an improved understanding of the role of the 'non-conventional' hemolysin and its associated RNA methyltransferase TlyA from H. pylori.

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The Cj0588 protein is a Campylobacter jejuni RNA methyltransferase

Cited by 12 publications

References 24 publications

Mechanisms and roles of the LuxS system, methyl recycling, and DNA methylation on the physiology of Campylobacter jejuni

Mechanisms and roles of the LuxS system, methyl recycling, and DNA methylation on the physiology of Campylobacter jejuni

Campylobacter

The Forgotten Virulence Factor: The ‘non-conventional’ Hemolysin TlyA And Its Role in Helicobacter pylori Infection

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