Visuo-spatial ability has been shown to be important to several aspects of laparoscopic performance, including simulator training. However, only a limited subset of visuo-spatial abilities has been used in such studies. Tests for different visuo-spatial ability factors differ greatly in stimulus complexity and the demands they make on speed of processing. To help clarify the involvement of visuo-spatial ability in laparoscopic performance the current study investigated the role of four visuo-spatial ability factors and two memory factors in different phases of surgical simulator training. Twenty four students participated in a two-month course, consisting of eight weekly, half-hour Laparoscopic simulator training sessions. Before the start of this course four visuo-spatial ability factors and two memory factors were measured. Simulator performance variables were (task) Duration, Motion efficiency, and Damage. Early learning (first three sessions) was characterized by significant improvements in all performance variables, while during late learning (last three sessions) only Duration continued to decrease. Late learning Damage fluctuated greatly. The involvement of visuo-spatial ability in laparoscopic training performance was found to be more complex than estimated before. The inconsistency of late learning Damage suggests an explicit damage criterion might be a well-advised addition for surgical simulator training, speed and efficiency being more natural goals in such settings.