The citron homology domain as a scaffold for Rho1 signaling

Bartual, S.G.; Wei, Wenfan; Zhou, Yao; Pravatà, Veronica M.; Fang, Wenxia; Yan, Kaizhou; Ferenbach, Andrew T.; Aalten, D.M.F. van

doi:10.1073/pnas.2110298118

Cited by 10 publications

(12 citation statements)

References 75 publications

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“…Dityrosine transporter 1 plays an essential role in spore wall maturation in Saccharomyces cerevisiae . Rho1 has been identified as an important regulator of the signaling pathway that regulated the compositions of the cell wall . All of these results clearly indicated that the cell wall of U.…”

Section: Resultsmentioning

confidence: 93%

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Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens

Fan,

Yu,

et al. 2023

J. Agric. Food Chem.

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Ustilaginoidea virens is a destructive phytopathogenic fungus that causes false smut disease in rice. In this study, the natural product 2,4-di-tert-butylphenol (2,4-DTBP) was found to be an environmentally friendly and effective agent for the first time, which exhibited strong antifungal activity against U. virens, with an EC50 value of 0.087 mmol/L. The scanning electron microscopy, fluorescence staining, and biochemical assays indicated that 2,4-DTBP could destroy the cell wall, cell membrane, and cellular redox homeostasis of U. virens, ultimately resulting in fungal cell death. Through the transcriptomic analysis, a total of 353 genes were significantly upregulated and 367 genes were significantly downregulated, focusing on the spindle microtubule assembly, cell wall and membrane, redox homeostasis, mycotoxin biosynthesis, and intracellular metabolism. These results enhanced the understanding of the antifungal activity and action mechanisms of 2,4-DTBP against U. virens, supporting it to be a potential antifungal agent for the control of false smut disease.

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Section: Resultsmentioning

confidence: 93%

“…44 Rho1 has been identified as an important regulator of the signaling pathway that regulated the compositions of the cell wall. 45 All of these results clearly indicated that the cell wall of U. virens underwent inhibition by 2,4-DTBP.…”

Section: ■ Materials and Methodsmentioning

confidence: 87%

Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens

Fan,

Yu,

et al. 2023

J. Agric. Food Chem.

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“…Our structural modeling showed that mNRK and cNRK CNH domains form a b-propeller structure similar to the prototypical CNH domain of yeast Rom2 (30). The mNRK CNH domain has evolved to acquire complete polybasic clusters and a larger positively charged surface in contact with the lipid bilayer.…”

Section: Molecular Evolution and Structure Of The Nrk Cnh Domainmentioning

confidence: 80%

The placental protein NRK promotes cell death through its plasma membrane-localizing CNH domain

Lestari,

Soda,

Moritsugu

et al. 2023

Preprint

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Placental development is regulated by the balance between cell proliferation and death. The placental protein NRK (NIK-related kinase) plays a role in preventing excessive placenta growth. We previously demonstrated that NRK underwent rapid molecular evolution in the ancestor of placental mammals and acquired the functional regions, including the phospholipid-binding citron homology (CNH) domain, by which NRK inhibits cell proliferation. NRK is also potentially responsible for cell death; caspases cleave NRK during apoptosis, releasing theC-terminal fragment that promotes cell death. Here, we explored the molecular mechanisms underlying the cell death-promoting effects of NRK. Our experimental data using HeLa, placenta trophoblast BeWo (human), and Rcho-1 (rat) cells indicated that the CNH domain of NRK was required and sufficient to promote cell death.In vitroandin silicostudies showed the NRK CNH domain bound to phospholipids via its polybasic clusters and remains at the plasma membrane (PM) during apoptosis. Evolutional analyses indicated that these clusters formed in the ancestor of placental mammals. Mutations in these clusters (CNH-18A) hindered the cell death-promoting activity of the CNH domain. We concluded that NRK promotes cell death through its plasma membrane-localizing CNH domain and suggested its active role in PM-associated events during cell death.

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“…The PH domain is distinguished from homologous PH domains by a loop insertion between strands β6 and β7 that packs against the CNH domain (Supplementary Figure S2B). The CNH domain forms a 7-bladed beta propeller with strongest homology to the CNH domain of yeast Rho1 (35). The CNH domain is held together by a beta strand contributed by the linker between the PH domain and blade 1 of the beta propeller (Figure 2B, yellow) and the C-terminus of the construct (Figure 2A, dark brown) which together form blade 7.…”

Section: Resultsmentioning

confidence: 99%

Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase

Truebestein

Waltenberger

Gehin

et al. 2022

Preprint

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Remodeling of the cytoskeleton underlies myriad processes essential for life. Protein kinases of the DMPK family are critical regulators of actomyosin contractility in cells. In the nematode worm, Caenorhabditis elegans, MRCK1 is required for the activation of myosin, leading to the development of cortical tension, apical constriction and early gastrulation. Here, we present the structure, conformation, and membrane-binding properties of C. elegans MRCK1. MRCK1 forms an obligate homodimer with N-terminal kinase domains, a parallel coiled-coil of 55 nm, and a C-terminal tripartite module of C1, PH and CNH domains. High-throughput liposome binding assays indicate binding to specific phosphoinositides, while the C-terminal Cdc42-binding (CRIB) motif binds specifically to activated Cdc42. The length of the coiled-coil domain of MRCK, as well as those of the related DMPK kinases ROCK, CRIK and DMPK, is remarkably conserved over millions of years of evolution, suggesting that they may function as molecular rulers to precisely position kinase activity at a fixed distance from the membrane.

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The citron homology domain as a scaffold for Rho1 signaling

Cited by 10 publications

References 75 publications

Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens

Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens

The placental protein NRK promotes cell death through its plasma membrane-localizing CNH domain

Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase

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