The cis/trans isomerisation of unsaturated fatty acids in Pseudomonas putida S12: An indicator for environmental stress due to organic compounds

Heipieper, Hermann J.; Loffeld, Burkhard; Keweloh, Heribert; Bont, J.A.M. de

doi:10.1016/0045-6535(95)00015-z

Cited by 119 publications

(102 citation statements)

References 21 publications

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“…AC30), and values of percentage of growth were reported by using growth in the absence of toxic as the 100%. For the measurement of the toxic effects, the investigated compounds showed above were added to exponentially growing cultures as described by Heipieper et al (1995). Growth inhibition caused by the toxicants was measured by comparing the differences in growth rate l (h -1 ) between intoxicated cultures (l, toxicant) with that of control cultures (l, control).…”

Section: Percentage Of Growth Rates Inhibition In the Presence Of Toxmentioning

confidence: 99%

Two naphthalene degrading bacteria belonging to the genera Paenibacillus and Pseudomonas isolated from a highly polluted lagoon perform different sensitivities to the organic and heavy metal contaminants

et al. 2009

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Section: Percentage Of Growth Rates Inhibition In the Presence Of Toxmentioning

confidence: 99%

Two naphthalene degrading bacteria belonging to the genera Paenibacillus and Pseudomonas isolated from a highly polluted lagoon perform different sensitivities to the organic and heavy metal contaminants

et al. 2009

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“…For the measurements of the toxic effects of the investigated compounds, toxins were added to exponentially growing, nonadapted cultures as described by Heipieper et al (10). Cultures were incubated in the presence of the toxins for 2 h in a shaking water bath at 30°C.…”

Section: Strains and Chemicalsmentioning

confidence: 99%

Prediction of the Adaptability of Pseudomonas putida DOT-T1E to a Second Phase of a Solvent for Economically Sound Two-Phase Biotransformations

Neumann

Kabelitz

Zehnsdorf

et al. 2005

Appl Environ Microbiol

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The strain Pseudomonas putida DOT-T1E was tested for its ability to tolerate second phases of different alkanols for their use as solvents in two-liquid-phase biotransformations. Although 1-decanol showed an about 10-fold higher toxicity to the cells than 1-octanol, the cells were able to adapt completely to 1-decanol only and could not be adapted in order to grow stably in the presence of a second phase of 1-octanol. The main explanation for this observation can be seen in the higher water and membrane solubility of 1-octanol. The hydrophobicity (log P) of a substance correlates with a certain partitioning of that compound into the membrane. Combining the log P value with the water solubility, the maximum membrane concentration of a compound can be calculated. With this simple calculation, it is possible to predict the property of an organic chemical for its potential applicability as a solvent for two-liquid-phase biotransformations with solventtolerant P. putida strains. Only compounds that show a maximum membrane concentration of less than 400 mM, such as 1-decanol, seem to be tolerated by these bacterial strains when applied in supersaturating concentrations to the medium. Taking into consideration that a solvent for a two-liquid-phase system should possess partitioning properties for potential substrates and products of a fine chemical synthesis, it can be seen that 1-decanol is a suitable solvent for such biotransformation processes. This was also demonstrated in shake cultures, where increasing amounts of a second phase of 1-decanol led to bacteria tolerating higher concentrations of the model substrate 3-nitrotoluene. Transferring this example to a 5-liter-scale bioreactor with 10% (vol/vol) 1-decanol, the amount of 3-nitrotoluene tolerated by the cells is up to 200-fold higher than in pure aqueous medium. The system demonstrates the usefulness of two-phase biotransformations utilizing solventtolerant bacteria.Biocatalysis using whole cells promised to play an important role in the industrial synthesis of fine chemicals, pharmaceuticals, and precursors for chemical syntheses. However, the number of successful processes using whole-cell biotransformations is very small so far because several factors limit the number of applications (29,30). One important limitation of a successful application of whole-cell biotechnological processes toward classical chemical synthesis is that many reactions of interest involve substrates or products that are extremely toxic for the bacteria (35). This problem can be solved by the application of an organic solvent phase that functions as a source and a sink for toxic organic substrates and products, respectively (36). For biotransformations with whole cells, the major advantage of an addition of a second organic phase lies within its ability to act as a sink for the substrates as well as in the continuous removal of products (19,21,36). In both cases, the presence of a solvent phase keeps the concentrations of substrates and toxins at a level that does not lead to toxic e...

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“…The analysis obtained from ANOVA showed that statistically the R. arrhizus UCP 402x mutant strain when compared to the wild R. arrhizus UCP 402, had a greater ability to grow in high pyrene concentrations (50 mg/L) in the three tested media. The growth inhibition was also demonstrated when Pseudomonas putida was inoculated in the medium containing glucose as the carbon source and octanol as toxically agent in the 0.6, 1.2, 1.8, and 2.4 mM concentration, being inversely proportional to the octanol concentration in the medium (Heipieper et al, 1995). The same result was displayed when Mycromycetes fungi grown in the presence of fluoranthene in the concentrations of 0.001, 0.01 and 0.1 g/L, showing a decrease in the mycelia growth as the concentration of the toxically compound was increased in the culture medium.…”

Section: Radial Growth Of R Arrhizus Ucp402 and R Arrhizus Ucp402x (mentioning

confidence: 99%

Monitoring the effect of pyrene on the germination and radial growth of the wild and mutant strains of Rhizopus arrhizus UCP402

Shiosaki

Albuquerque

Okada

et al. 2008

Braz. arch. biol. technol.

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The cis/trans isomerisation of unsaturated fatty acids in Pseudomonas putida S12: An indicator for environmental stress due to organic compounds

Cited by 119 publications

References 21 publications

Two naphthalene degrading bacteria belonging to the genera Paenibacillus and Pseudomonas isolated from a highly polluted lagoon perform different sensitivities to the organic and heavy metal contaminants

Two naphthalene degrading bacteria belonging to the genera Paenibacillus and Pseudomonas isolated from a highly polluted lagoon perform different sensitivities to the organic and heavy metal contaminants

Prediction of the Adaptability of Pseudomonas putida DOT-T1E to a Second Phase of a Solvent for Economically Sound Two-Phase Biotransformations

Monitoring the effect of pyrene on the germination and radial growth of the wild and mutant strains of Rhizopus arrhizus UCP402

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