1975
DOI: 10.1016/0002-9378(75)90005-8
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The circulating vasopressinase of pregnancy: Species comparison with radioimmunoassay

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Cited by 56 publications
(25 citation statements)
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“…5). It is noteworthy that although the serum of pregnant woman was found to contain increasingly large amounts of vasopressinase activity as gestation advances, umbilical cord plasma and amniotic fluid have several orders of magnitude less activity (27). Vasopressinase activity, a measure of the degradation of vasopressin, is not detectable in the plasma or amniotic fluid of sheep (27).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…5). It is noteworthy that although the serum of pregnant woman was found to contain increasingly large amounts of vasopressinase activity as gestation advances, umbilical cord plasma and amniotic fluid have several orders of magnitude less activity (27). Vasopressinase activity, a measure of the degradation of vasopressin, is not detectable in the plasma or amniotic fluid of sheep (27).…”
Section: Discussionmentioning
confidence: 99%
“…It is noteworthy that although the serum of pregnant woman was found to contain increasingly large amounts of vasopressinase activity as gestation advances, umbilical cord plasma and amniotic fluid have several orders of magnitude less activity (27). Vasopressinase activity, a measure of the degradation of vasopressin, is not detectable in the plasma or amniotic fluid of sheep (27). In the absence of intrinsic mechanisms for enzymatic degradation we found that increased concentrations of vasopressin in sheep amniotic fluid persist for at least 24 h; thus, we have data confirming the validity of the measurement of vasopressin as an index of fetal hypoxia in sheep.…”
Section: Discussionmentioning
confidence: 99%
“…Dosages are indicated on the righl. pregnant woman was found to contain increasingly large amounts of vasopressinase activity as gestation advances, umbilical cord plasma and amniotic fluid have several orders of magnitude less activity (27). Vasopressinase activity, a measure of the degradation of vasopressin, is not detectable in the plasma or amniotic fluid of sheep (27).…”
Section: Discussionmentioning
confidence: 99%
“…pregnant woman was found to contain increasingly large amounts of vasopressinase activity as gestation advances, umbilical cord plasma and amniotic fluid have several orders of magnitude less activity (27). Vasopressinase activity, a measure of the degradation of vasopressin, is not detectable in the plasma or amniotic fluid of sheep (27). In the absence of intrinsic mechanisms for enzymatic degradation, we found that increased concentrations of vasopressin in sheep amniotic fluid persist for at least 24 h. Thus, we have data confirming the validity of the measurement of vasopressin as an index of fetal hypoxia in sheep.…”
Section: Discussionmentioning
confidence: 99%
“…Most groups have employed 125 1 and the chloramine-T method and have experienced difficulties in obtaining a high specific activity product with some enzymes. For example, it proved difficult to 1:5.000 I:40,000 I:20,000 I:10,000 for plasma angiotensinase: based on RIA of angiotensin I (Strong et al, 1972) for plasma vaspressinase: based on RIA of vasopressin (Rosenbloom et al, 1975) Combine problems of biological assays and of the RIA used for endpoint detection maintain their catalytic activity during isolation procedures, certain others such as the brain isoenzyme of creatine kinase (CK), are partially inactivated during purification (Keutel et al, 1972). This does not necessarily preclude their use as immunogens, though by preference one should always attempt to maintain enzymic activity and thereby guarantee the integrity of the total tertiary structure; as Sela (1969) has pointed out, "antibodies to native proteins are derived mostly against conformational rather than sequential determinants."…”
Section: Categories Of Analytical Techniquesmentioning
confidence: 99%