THE CIRCULATING RECOMBINANT FORM RF2k/1b OF HEPATITIS C VIRUS: PROBLEMS IN DIAGNOSTICS AND THERAPY

Дементьева, Наталья Евгеньевна; Kalinina, Olga; Знойко, О О; Беляков, Н. А.; Жебрун, А Б

doi:10.22328/2077-9828-2016-8-1-42-52

Cited by 2 publications

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“…Анализ эпидемиологических данных показал, что пациент (323) являлся в прошлом потребителем инъекционных наркотиков, а предполагаемый путь инфицирования пациентки (4120) -половой от супруга, который также употреблял внутривенно наркотические препараты. Пациентка (5101), у которой была обнаружена рекомбинантная форма RF2k/1b, имела в анамнезе многочисленные оперативные вмешательства и ранее проживала в Грузии, где распространенность этого варианта достаточно высока [1].…”

Section: рисунок 3 филогенетический анализ нуклеотидных последовательностей области Ns5bunclassified

“…Рутинные методы генотипирования, используемые в настоящее время в клинических лабораториях, не позволяют определять рекомбинантные варианты вируса, поскольку в коммерческих тест-системах для идентификации генотипов в качестве мишени используется только один фрагмент вируса 5′UTR/core. Этого недостаточно для определения рекомбинантных форм [1].…”

Section: рисунок 3 филогенетический анализ нуклеотидных последовательностей области Ns5bunclassified

“…В 2002 г. в Санкт-Петербурге был обнаружен первый межгенотипный рекомбинантный вариант HCV -RF2k/1b, структурная часть генома которого образована субтипом 2k, а неструктурная -субтипом 1b вируса [14,15]. В 2005 г. рекомбинантная форма RF2k/1b была включена в классификацию HCV как отдельная номенклатурная единица -RF 01_1b2k (рекомбинантная форма), а в 2009 -как CRF 01_1b2k (циркулирующая рекомбинантная форма) [1,16,21]. В настоящее время это единственный рекомбинантный вариант HCV, который широко распространен в мире и имеет эпидемиологическое значение.…”

Section: Introductionunclassified

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Genetic diversity of hepatitis C virus in Nanaian region, Khabarovsk territory

Котова¹,

Балахонцева²,

Базыкина³

et al. 2021

Russian Journal of Infection and Immunity

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Examining hepatitis C virus (HCV) genetic diversity is of great practical value in molecular-epidemiological research, development of specific prevention tools and outlining therapeutic strategy. Aim of study: to conduct analysis assessing HCV genetic diversity circulating in the Nanaisky region population of the Khabarovsk territory. Materials and Methods. Molecular and genetic analysis of 124 blood plasma samples collected from patients with chronic hepatitis C and residing in the Nanaisky region was conducted. Results. HCV RNA was detected in 84 (67.7±4.2%) plasma samples. HCV genotyping was performed by using AmpliSens – 1/2/3 kit (Central Research Institute of Epidemiology, Moscow, Russian Federation) showing that genotype 3 dominated reaching up to 47.6±5.4% (n=40). Genotype 1 was detected in 30 patients (35.7±5.2%). In thirteen cases (15.5±3.9%) genotype 2 was identified, whereas in one case (1.2±1.2%) virus genotype was unidentified. Phylogenetic analysis of the nucleotide sequences in HCV NS5B region was performed for 60 HCV RNA-positive samples showing subtype ratio as follows: 1a - 2 (3.3±2.3%), 1b - 23 (38.3±6.3%), 2а – 6 (10.0±3.9%), 2с – 2 (3.3±2.3%), 3а – 27(45.0±6.4%). Three samples of RF2k/1b recombinant virus were found. A full NS2 gene nucleotide sequence was cloned in order to confirm the recombination event. The results of the study evidence about a need to conduct multi-layered examination of patients with chronic hepatitis C by using current molecular and biologic methods for assigning proper therapy coupled to characteristics of the isolated strains. The data regarding hepatitis C virus molecular and genetic parameters circulating in the Far Eastern Federal District, Russia, are rather limited. Hence, our study would contribute to current understanding of HCV genovariants circulating in territories of the Russian Federation.

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Section: рисунок 3 филогенетический анализ нуклеотидных последовательностей области Ns5bunclassified

Section: Introductionunclassified

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Genetic diversity of hepatitis C virus in Nanaian region, Khabarovsk territory

Котова¹,

Балахонцева²,

Базыкина³

et al. 2021

Russian Journal of Infection and Immunity

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Detection of circulating HCV recombinant form RF1_2k/1b in blood serum of patients by real-time RT-PCR

et al. 2021

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Globally, about 70 million people are infected with the hepatitis C virus (HCV), and about 400 thousand people die annually from chronic hepatitis C complications. The management of patients with chronic hepatitis C may require HCV genotyping, since the efficiency of some widely used antiviral drugs strongly depend on the viral genotype and/or subtype. The most prevalent HCV circulating recombinant form, RF1_2k/1b, is misclassified as genotype 2 by many commercial HCV genotyping kits, based on the RT-PCR analysis of the 5’ untranslated region of the HCV genome. This leads to inappropriate patient treatment, since the accepted treatment schemes for HCV genotype 2 are ineffective for the RF1_2k/1b. Here we describe a method for detecting the RNA HCV RF1_2k/1b in blood samples by RT-PCR analysis of two regions in HCV genome (5’UTR and NS5b). The method was tested on 240 blood serum samples from HCV infected patients, in which HCV genotype was defined as 2 or mixed (2+1 or 2+3) by the two commercial genotyping kits “OT-Hepatogen-C genotype” (“DNA-Technology”, Moscow) and “RealBest RNA HCV-1/2/3” (“Vector- Best “, Novosibirsk). 50 (20.8%) RF1_2k/1b cases were revealed, including three mixed infections: RF1_2k/1b + 1a, RF1_2k/1b + 3a, RF1_2k/1b + 1b. In all cases, the accuracy of HCV typing by the proposed method was confirmed by Sanger sequencing and phylogenetic analysis. The method is easy to implement into clinical practice and may be used in clinical settings equipped for RT-PCR analysis to correctly identify the recombinant variant RF1_2k/1b.

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THE CIRCULATING RECOMBINANT FORM RF2k/1b OF HEPATITIS C VIRUS: PROBLEMS IN DIAGNOSTICS AND THERAPY

Cited by 2 publications

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Genetic diversity of hepatitis C virus in Nanaian region, Khabarovsk territory

Genetic diversity of hepatitis C virus in Nanaian region, Khabarovsk territory

Detection of circulating HCV recombinant form RF1_2k/1b in blood serum of patients by real-time RT-PCR

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