The circRNA-0001361/miR-491/FGFR4 axis is associated with axillary response evaluated by ultrasound following NAC in subjects with breast cancer

Sun, Junjie; Li, Lin; Chen, Xiaoran; Yang, Chunfeng; Wang, Li

doi:10.1016/j.bbrep.2023.101481

Cited by 1 publication

(2 citation statements)

References 51 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24 CircRNAs are long noncoding RNAs (lncRNAs) with the characteristic feature of a covalent close-loop structure lacking 5′ and 3′ ends. [25][26][27][28] There is emerging evidence of circRNAs that possess a miRNA response element (MRE) and function as competing endogenous RNAs (ceRNAs) that sponge and sequester the endogenous activity of miRNAs. For instance, a study by Sun et al reported that upregulation of circRNA-0001361 increased the expression of FGFR4, a potential epithelial-to-mesenchymal transition and metastasis inducer, by sponging miR-491-5p.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, a study by Sun et al reported that upregulation of circRNA-0001361 increased the expression of FGFR4, a potential epithelial-to-mesenchymal transition and metastasis inducer, by sponging miR-491-5p. 28 Silencing of this circRNA downregulated the expression of FGFR4, reducing the axillary response after neoadjuvant chemotherapy in BC. A study by Song et al showed that the upregulation of circ_ATAD3B sponged miR-570-3p, which significantly increased the expression level of MX2, a gene linked to the progression of human cancer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transforming Growth Factor-β1-mediated Regulation of circ_DISP3 and ATF3 in Human Triple-negative Breast Cancer Cells

Mohan,

Bharathy,

Induja

et al. 2023

Gene Expr

View full text Add to dashboard Cite

Background and objectives: Transforming growth factor-beta 1 (TGF-β1) stimulates activating transcription factor 3 (ATF3), and it is required for the expression of cell proliferation, invasion, and bone metastasis genes in human breast cancer cells. Noncoding RNAs have a crucial role in both physiological and pathological circumstances in the expression of genes. We previously determined the direct targeting of ATF3 by miR-4638-3p, downregulated by TGF-β1 in human triple-negative breast cancer cells (MDA-MB231). This study identified the molecular mechanism of downregulating ATF3 targeting miR-4638-3p via circRNA in MDA-MB231 cells. Methods:In-silico analyses were used to identify the list of circRNAs targeting miR-4638-3p. RT-qPCR was performed to determine the expression of shortlisted circRNAs in MDA-MB231 cells. Transient transfection and western blot analyses were carried out to identify the functional role of circ_DISP3 in MDA-MB231 cells. A dual-luciferase reporter assay was used to identify the direct binding of circ_DISP3 and miR-4638-3p. Results:There was an inverse correlation between the expression of circ_DISP3 and miR-4638-3p in these cells. Antisense oligosmediated silencing of circ_DISP3 restored the function of miR-4638-3p, resulting in the downregulation of ATF3 in MDA-MB231 cells. Furthermore, a luciferase reporter assay identified the direct binding of circ_DISP3 toward miR-4638-3p in these cells.Conclusions: TGF-β1 influenced circ_DISP3 to sponge miR-4638-3p, promoting ATF3 expression in MDA-MB231 cells. Silencing this circRNA caused the restoration of miR-4638-3p activity toward targeting ATF3 in these cells. Hence, we suggest that the

show abstract