2019
DOI: 10.1038/s41467-019-08299-7
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The circadian clock components BMAL1 and REV-ERBα regulate flavivirus replication

Abstract: The circadian clock regulates immune responses to microbes and affects pathogen replication, but the underlying molecular mechanisms are not well understood. Here we demonstrate that the circadian components BMAL1 and REV-ERBα influence several steps in the hepatitis C virus (HCV) life cycle, including particle entry into hepatocytes and RNA genome replication. Genetic knock out of Bmal1 and over-expression or activation of REV-ERB with synthetic agonists inhibits the replication of HCV and the related flaviru… Show more

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Cited by 80 publications
(68 citation statements)
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“…This is of high significance as it not only identifies REV-ERB as an important cold-induced gene but it also demonstrates that massive upregulation of REV-ERB is sufficient to control phase resetting in mammalian cells. Finally, given that REV-ERB is a key chromatin regulator linking circadian rhythms with metabolism (Cho et al, 2012;Zhang et al, 2015), inflammation (Pariollaud et al, 2018) and cardiac surgery outcomes (Montaigne et al, 2018), and has tumor suppressor (Sulli et al, 2018) and anti-viral properties (Zhuang et al, 2019), the recognition that REV-ERB expression can be boosted more than ten-fold by 18°C exposure will be of significance for the development of novel strategies that broaden the applications of controlled deep hypothermia.…”
Section: Discussionmentioning
confidence: 99%
“…This is of high significance as it not only identifies REV-ERB as an important cold-induced gene but it also demonstrates that massive upregulation of REV-ERB is sufficient to control phase resetting in mammalian cells. Finally, given that REV-ERB is a key chromatin regulator linking circadian rhythms with metabolism (Cho et al, 2012;Zhang et al, 2015), inflammation (Pariollaud et al, 2018) and cardiac surgery outcomes (Montaigne et al, 2018), and has tumor suppressor (Sulli et al, 2018) and anti-viral properties (Zhuang et al, 2019), the recognition that REV-ERB expression can be boosted more than ten-fold by 18°C exposure will be of significance for the development of novel strategies that broaden the applications of controlled deep hypothermia.…”
Section: Discussionmentioning
confidence: 99%
“…The dry extract was re-suspended in chloroform/MeOH (1:1). Phosphatidylethanolamine (PE) and phosphatidylserine (PS) were separated by liquid chromatography using a Shimadzu XR system (Shimadzu, Kyoto, Japan) 44 . PE and PS were then detected using an Orbitrap Elite mass spectrometer in full scan mode with a mass range of 400-1000 m/z at a target resolution of 240,000 (FWHM at m/z 400).…”
Section: Methodsmentioning
confidence: 99%
“…Furthermore, in liver biopsies from HCV genotype 1b-infected patients, PER2 was almost exclusively localized to the nucleus, which is suggestive of an auto-inhibitory transcriptional feedback loop [79]. More recently, it was shown that the core-clock components BMAL1 and REV-ERBα act upon more steps in the HCV life cycle, including the entry of viral particles into hepatocytes and the replication of the viral RNA [89]. In fact, pharmacological activation of REV-ERB inhibited HCV entry, and genetic knockout of Bmal1 or overexpression/pharmacological activation of REV-ERB was able to counteract HCV replication by modulating the circadian expression of the liver-specific microRNA miR-122 with consequent derangements of signalling pathways involved in lipid metabolism [89].…”
Section: Epigenetic Mechanisms Underlying the Interaction Between Virmentioning
confidence: 96%