The circadian clock and darkness control natural competence in cyanobacteria

Taton, Arnaud; Erikson, Christian; Yang, Yiling; Rubin, Benjamin E. R.; Rifkin, Scott A.; Golden, James W.; Golden, Susan S.

doi:10.1101/822627

Cited by 21 publications

(53 citation statements)

References 65 publications

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“…The genetic cluster encoding the six pilin-like proteins ( Fig 1C and 1D) likely produces three types of pili that use the same transmembrane pilus structure. Based on homology with the annotated genes from S. elongatus 16 conserved domains found using the CD-search tool in NCBI, we suggest the three pili types: PilA1-PilE, PilA2-PilV and PilA3-PilW ( Fig 1C). Of these types, shotgun proteomic analyses have only ever detected PilA1-PilE 19,20 , although PilA2 was also detected in low abundance in cellular -but not extracellularproteomic datasets 22 .…”

Section: Fig 1 | Pilus In the Marine Cyanobacterium Synechococcus Spmentioning

confidence: 97%

“…(E) Synechococcus sp. WH7803 structural pilus proteins identified by homology with S. elongatus PCC 7942 16 and assembled as modelled by Craig et al 11 .…”

Section: Fig 1 | Pilus In the Marine Cyanobacterium Synechococcus Spmentioning

confidence: 99%

“…Using the pilus apparatus from the freshwater cyanobacterium S. elongatus PCC 7942 as a reference 16 and the established architecture for type IV pilus machinery 11 , we were able to find all components necessary for pilus assembly in Synechococcus sp. WH7803 ( Fig 1E).…”

Section: Fig 1 | Pilus In the Marine Cyanobacterium Synechococcus Spmentioning

confidence: 99%

“…This latter function requires an additional set of proteins for competence such as ComEA and ComEC. While acquisition of DNA by S. elongatus is performed by the third of three PilA-like proteins encoded by this strain (PilA3) 16 , no known function has been attributed to PilA1 and PilA2 other than being dispensable for attachment and biofilm formation 17 . A mutant in S. elongatus that no longer produced PilB -the protein responsible for pilus elongation-abolished the production of pili appendages, made up of PilA1, and was reported to suppress planktonic growth of this strain .…”

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confidence: 98%

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Pili allow dominant marine cyanobacteria to avoid sinking and evade predation

Christie-Oleza

Aguiló-Ferretjans

Bosch

et al. 2020

Preprint

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How oligotrophic marine cyanobacteria position themselves in the water column is currently unknown. The current paradigm is that these organisms avoid sinking due to their reduced size and passive drift within currents. Here, we show that one in four picocyanobacteria encode a type IV pilus which allows these organisms to increase drag and remain suspended at optimal positions in the water column, as well as evade predation by grazers. The evolution of this sophisticated floatation mechanism in these purely planktonic streamlined microorganisms has profound implications for our current understanding of microbial distribution in the oceans, predator-prey interactions and, ultimately, will influence future models of carbon flux dynamics in the oceans.

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Section: Fig 1 | Pilus In the Marine Cyanobacterium Synechococcus Spmentioning

confidence: 97%

“…(E) Synechococcus sp. WH7803 structural pilus proteins identified by homology with S. elongatus PCC 7942 16 and assembled as modelled by Craig et al 11 .…”

Section: Fig 1 | Pilus In the Marine Cyanobacterium Synechococcus Spmentioning

confidence: 99%

Section: Fig 1 | Pilus In the Marine Cyanobacterium Synechococcus Spmentioning

confidence: 99%

mentioning

confidence: 98%

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Pili allow dominant marine cyanobacteria to avoid sinking and evade predation

Christie-Oleza

Aguiló-Ferretjans

Bosch

et al. 2020

Preprint

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“…Modes of horizontal gene transfer (HGT) available to Prochlorococcus are lipid-bound vesicles, which contain DNA and are known to be abundant in the oceans [10], [11] , natural transformation, but only in some LLIV clades that contain the necessary genes for competence [12] , and transduction via cyanophage capsids. The latter is one of the canonical modes of HGT in bacteria [13]- [15] and occurs at significant frequencies in aquatic ecosystems [16]- [18] .…”

Section: Introductionmentioning

confidence: 99%

Frequency of mispackaging ofProchlorococcusDNA by cyanophage

Raho

Laurenceau

Forget

et al. 2020

Preprint

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Prochlorococcus cells are the numerically dominant phototrophs in the open ocean. Cyanophages that infect them are thus a notable fraction of the total viral population in the euphotic zone, and, as vehicles of horizontal gene transfer, appear to drive their evolution. Here we examine the propensity of three cyanophages -a podovirus, a siphovirus, and a myovirus -to mispackage host DNA in their capsids while infecting Prochlorococcus , the first step in phage-mediated horizontal gene transfer. We find the mispackaging frequencies are distinctly different among the three phages. Myoviruses mispackage host DNA at low and stable frequencies, while podo-and siphoviruses vary in their mispackaging frequencies by orders of magnitude depending on growth light intensity. We attribute this difference to the concentration of intracellular reactive oxygen species and protein synthesis rates. Based on our findings, we propose a model of mispackaging frequency determined by the imbalance between the production of capsids and the number of phage genome copies during infection. RESULTS AND DISCUSSIONHost DNA mispackaging frequency in cyanophage capsids. Using six different loci on the Prochlorococcus MED4 chromosome (representative of the entire chromosome, see Table 3), we quantified the level of mispackaging of MED4 DNA in the capsids of the three different cyanophages (Fig. 1A, Table 2) during infection (Fig. 1B, C). The packaging strategy of the podovirus is T7-like [26] , the myovirus' is likely T4-like headful packaging [27] while the siphovirus' is unknown, but 490 bp direct terminal repeats suggest a T7-like packaging mechanism [28] . Host DNA was detected in the capsids of all 3 cyanophages (Fig. 1C). The levels of mispackaging are low compared to those reported for high transducing phages in S. aureus [29] for example, but comparable to levels (~10 PPM) reported for the marine Synechococcus cyanophage S-PM2 [30] . Corresponding gene Putative functionPrimer sequences (forward and reverse, 5'-3')

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Solution NMR structure of Se0862, a highly conserved cyanobacterial protein involved in biofilm formation

et al. 2020

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Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a fourstranded β-sheet that is flanked by four α-helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs.

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The circadian clock and darkness control natural competence in cyanobacteria

Cited by 21 publications

References 65 publications

Pili allow dominant marine cyanobacteria to avoid sinking and evade predation

Pili allow dominant marine cyanobacteria to avoid sinking and evade predation

Frequency of mispackaging ofProchlorococcusDNA by cyanophage

Solution NMR structure of Se0862, a highly conserved cyanobacterial protein involved in biofilm formation

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