The cin Quorum Sensing Locus of Rhizobium etli CNPAF512 Affects Growth and Symbiotic Nitrogen Fixation

Daniels, Ruth; Vos, Dirk De; Desair, J.; Raedschelders, Gert; Luyten, Ellen; Rosemeyer, Viola; Verreth, Christel; Schoeters, E.; Vanderleyden, Jozef; Michiels, Jan

doi:10.1074/jbc.m106655200

Cited by 124 publications

(105 citation statements)

References 36 publications

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“…This sacB suicide vector was used to introduce the raiR mutant allele into FAJ4007. The reporter fusions used are cinI-gusA (pFAJ4014) (19) and raiI-gusA (pFAJ1458) (E. Luyten, personal communication). A plasmid-borne P nptII -cinI fusion was constructed in the vector pFAJ1709 (38).…”

Section: Methodsmentioning

confidence: 99%

“…A plasmid-borne P nptII -cinI fusion was constructed in the vector pFAJ1709 (38). For this, the 1.4-kbp HindIII-BamHI fragment of pFAJ4004 containing cinI (19) was cloned into the HpaI site of pFAJ1709 after blunting of the fragments, yielding plasmid pCMPG8798. Plasmids were introduced into CNPAF512 by conjugation (19).…”

Section: Methodsmentioning

confidence: 99%

“…R. etli strains include WT CNPAF512 (37), a cinI mutant (FAJ4006) (19), a cinR mutant (FAJ4007) (19), and a raiI mutant (FAJ1328) (18). A cinRraiR mutant (CMPG8299) was constructed.…”

Section: Methodsmentioning

confidence: 99%

“…R. etli CNPAF512 produces at least seven different QS signal molecules, produced by the cinIR and raiIR QS system. cinI and raiI code for the AHL synthases, and cinR and raiR code for the transcriptional regulators that bind the AHLs (18,19). Recently, it was shown that both QS systems in R. etli are clearly responsive to distinct AHLs.…”

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confidence: 99%

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Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli

Daniels

Reynaert

Hoekstra

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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138

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Swarming motility is suggested to be a social phenomenon that enables groups of bacteria to coordinately and rapidly move atop solid surfaces. This multicellular behavior, during which the apparently organized bacterial populations are embedded in an extracellular slime layer, has previously been linked with biofilm formation and virulence. Many population density-controlled activities involve the activation of complex signaling pathways using small diffusible molecules, also known as autoinducers. In Gramnegative bacteria, quorum sensing (QS) is achieved primarily by means of N-acylhomoserine lactones (AHLs). Here, we report on a dual function of AHL molecules in controlling swarming behavior of Rhizobium etli, the bacterial symbiotic partner of the common bean plant. The major swarming regulator of R. etli is the cinIR QS system, which is specifically activated in swarming cells by its cognate AHL and other long-chain AHLs. This signaling role of long-chain AHLs is required for high-level expression of the cin and rai QS systems. Besides this signaling function, the long-chain AHLs also have a direct role in surface movement of swarmer cells as these molecules possess significant surface activity and induce liquid flows, known as Marangoni flows, as a result of gradients in surface tension at biologically relevant concentrations. These results point to an as-yet-undisclosed direct role of long-chain AHL molecules as biosurfactants.cell-cell signaling ͉ motility ͉ quorum sensing

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…R. etli strains include WT CNPAF512 (37), a cinI mutant (FAJ4006) (19), a cinR mutant (FAJ4007) (19), and a raiI mutant (FAJ1328) (18). A cinRraiR mutant (CMPG8299) was constructed.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Quorum signal molecules as biosurfactants affecting swarming in Rhizobium etli

Daniels

Reynaert

Hoekstra

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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138

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“…The importance of AHLs has been demonstrated in diverse fields, including agriculture, ecology, and medicine, with roles that range from the establishment of mutualistic or symbiotic relationships to their importance in virulent and pathogenic interactions (Daniels et al, 2002;Ortíz-Castro et al, 2008;Atkinson and Williams, 2009;Williams and Cámara, 2009). Recent evidence indicates that plants are able to perceive AHLs; the application of AHLs to Arabidopsis or Medicago truncatula results in distinct transcriptional changes in the roots and shoots and affects primary root growth, lateral root formation, and root hair development (Mathesius et al, 2003;Ortíz-Castro et al, 2008;von Rad et al, 2008;Morquecho-Contreras et al, 2010).…”

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confidence: 99%

N-3-Oxo-Decanoyl-l-Homoserine-Lactone Activates Auxin-Induced Adventitious Root Formation via Hydrogen Peroxide- and Nitric Oxide-Dependent Cyclic GMP Signaling in Mung Bean

et al. 2011

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N-Acyl-homoserine-lactones (AHLs) are bacterial quorum-sensing signaling molecules that regulate population density. Recent evidence demonstrates their roles in plant defense responses and root development. Hydrogen peroxide (H 2 O 2 ), nitric oxide (NO), and cyclic GMP (cGMP) are essential messengers that participate in various plant physiological processes, but how these messengers modulate the plant response to N-acyl-homoserine-lactone signals remains poorly understood. Here, we show that the N-3-oxo-decanoyl-homoserine-lactone (3-O-C10-HL), in contrast to its analog with an unsubstituted branch chain at the C3 position, efficiently stimulated the formation of adventitious roots and the expression of auxin-response genes in explants of mung bean (Vigna radiata) seedlings. This response was mimicked by the exogenous application of auxin, H 2 O 2 , NO, or cGMP homologs but suppressed by treatment with scavengers or inhibitors of H 2 O 2 , NO, or cGMP metabolism. The 3-O-C10-HL treatment enhanced auxin basipetal transport; this effect could be reversed by treatment with H 2 O 2 or NO scavengers but not by inhibitors of cGMP synthesis. Inhibiting 3-O-C10-HL-induced H 2 O 2 or NO accumulation impaired auxin-or 3-O-C10-HL-induced cGMP synthesis; however, blocking cGMP synthesis did not affect auxin-or 3-O-C10-HLinduced H 2 O 2 or NO generation. Additionally, cGMP partially rescued the inhibitory effect of H 2 O 2 or NO scavengers on 3-O-C10-HL-induced adventitious root development and auxin-response gene expression. These results suggest that 3-O-C10-HL, unlike its analog with an unmodified branch chain at the C3 position, can accelerate auxin-dependent adventitious root formation, possibly via H 2 O 2 -and NO-dependent cGMP signaling in mung bean seedlings.

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