The Ciliary Activity of the Middle Ear Lining-functional and Morphological Observation

Ōhashi, Y.; Nakai, Yousuke; Kihara, Shoko; Maruoka, Kenichi; Ikeoka, Hiroshi; Uemura, Yoshinori

doi:10.1016/s0385-8146(85)80122-x

Cited by 20 publications

(1 citation statement)

References 9 publications

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“…The middle ear is a part of respiratory mucosa, which has mucociliary system that has a crucial role in clearing the tympanic cavity [16]. The present paper showed that the ciliary activity of the middle ear mucosa in rabbits experimentally induced AOM was short, impaired and disrupted.…”

Section: Discussionsupporting

confidence: 46%

Effects of Otitis Media on the Eustachian Tube: An Animal Experiment

Sevil¹

2020

GJO

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Objective: To study the effect of the acute otitis media (AOM) on the middle ear mucosa and auditory tube in rabbits and to evaluate the results histologically. Material Methods:The study was carried out on 35 adult rabbits (New Zealand white rabbits), weighing 1500-1800 grams. The middle ear was inoculated with a bacterial suspension containing S. aureus to induce acute otitis media. Based on the exposure time of 1, 2, 4, 12, 24 weeks, the animals were classified into five groups. The rabbits in the acute otitis media group were killed after they were exposed for 1 and 2 weeks (acute), four weeks (subacute), or 12 and 24 weeks (chronic), and they were compared with the control group. The left control middle ear, the right inoculated bulla and the eustachian tube were removed. Sections of the temporal bones were dissected on block by covering the middle ear cavity and nasopharynx passing through the eustachian tube. Results:The goblet cells in the group AOM after one week was as dense as those in the control group. There was no difference between the groups in subepithelial edema, the number of eosinophils, subepithelial vasodilatation, intraepithelial gland formation and, subepithelial gland formation (p>0.05). The gland volume and the goblet cells density were extended to peak two weeks after the inoculation since there was primarily a volume increase in the components of the mucous gland. There was a significant increase in the volume of the gland layer within at least 12 weeks after the inoculation of the bacterial middle ear, primarily since there was an increase in a volume of the components of the mucous gland. Although there was a decrease in the volume 12 weeks after acute infection, it was completely normalized after 24 weeks. Conclusion:Acute otitis media directly affects the auditory tube. Besides, excessive mucus secretion and goblet cell proliferation included histologic changes of auditory tube mucosa

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Section: Discussionsupporting

confidence: 46%

Effects of Otitis Media on the Eustachian Tube: An Animal Experiment

Sevil¹

2020

GJO

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Acute effects of isopropyl alcohol exposure on the middle ear mucosa

Ōhashi

Nakai

Ikeoka

et al. 1987

J of Applied Toxicology

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Guinea pigs were exposed to 400 ppm or 5500 ppm of isopropyl alcohol (IPA) for 24 successive hours, and then middle ear mucosal samples in both proximal and distal sites to the eustachian tube were obtained for functional and morphological examinations. The ciliary activity was examined according to the photoelectric method of Ohashi and Nakai. The morphology of the middle ear lining was examined by transmission electron microscopy. The results showed that IPA at the allowable level of 400 ppm has an acute effect on the mucociliary system of the middle ear mucosa. Recovery from damage occurs within two weeks. At higher levels of exposure to IPA (more than 10 times the allowable level) moderate deterioration of the ciliary activity and severe damage of epithelial cells were observed and recovery within two weeks was not seen. It is concluded that IPA at the allowable level of 400 ppm does not cause significant middle ear disorders, but workers exposed to higher levels of IPA should be given careful otolaryngological examinations and follow-up observations.

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An experimental study on the respiratory toxicity of isopropyl alcohol

Ōhashi

Nakai

Ikeoka

et al. 1988

J of Applied Toxicology

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Guinea-pigs were exposed to 400 ppm or 5500 ppm of isopropyl alcohol (IPA) for 24 successive hours, and they were killed immediately after the exposure period. The ciliary activities of the nose and trachea were examined. In addition, the epithelium of the nose and trachea was observed by scanning and transmission electron microscopy. The present study showed that exposure to 400 or 5500 ppm of IPA vapor caused deterioration of the ciliary activity and also some pathological changes. Although our study revealed that exposure to 400 ppm of IPA vapor can affect the mucosa of the nose and trachea, the ciliary activity of the 400-ppm exposure group was not too poor and morphological changes were rather mild. Recovery from such degeneration might be rapid. Therefore, our study supports the justification of the allowable IPA level recommended by ACGIH.

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The Ciliary Activity of the Middle Ear Lining-functional and Morphological Observation

Cited by 20 publications

References 9 publications

Effects of Otitis Media on the Eustachian Tube: An Animal Experiment

Effects of Otitis Media on the Eustachian Tube: An Animal Experiment

Acute effects of isopropyl alcohol exposure on the middle ear mucosa

An experimental study on the respiratory toxicity of isopropyl alcohol

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