The CIC library: a large insert YAC library for genome mapping in <i>Arabidopsis thaliana</i>

Creusot, Francine; Fouilloux, Eric; Dron, Michel; Lafleuriel, J.; Picard, Georges; Billault, Alain; Paslier, Denis Le; Cohen, Daniel; Chabouté, Marie‐Edith; Durr, Andrée; Fleck, Jacqueline; Gigot, Claude; Camilleri, Christine; Bellini, Catherine; Caboche, Michel; Bouchez, David

doi:10.1046/j.1365-313x.1995.08050763.x

Cited by 171 publications

(130 citation statements)

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“…CSD1 was identified on TAMUBAC10I10, 20M22, and 30M24. These BACs are coincident with the CICYAC8C10, placing CSD1 at the top of chromosome 1 between markers PAI1 and PHYA (http://cbil.humgen.upenn.edu/ϳatgc/physicalmapping; Creusot et al, 1995). This location agrees with the position identified for the genomic sequence for CSD1 in the Arabidopsis genome project (http://genome-www3.…”

Section: Map Positions Of the Known Sod Structural Genessupporting

confidence: 78%

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Superoxide Dismutase in Arabidopsis: An Eclectic Enzyme Family with Disparate Regulation and Protein Localization

1998

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A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD1, CSD2, and CSD3), three FeSODs (FSD1, FSD2, and FSD3), and one MnSOD (MSD1). The chromosomal location of these seven SOD genes has been established. To study this enzyme family, antibodies were generated against five proteins: CSD1, CSD2, CSD3, FSD1, and MSD1. Using these antisera and nondenaturing-polyacrylamide gel electrophoresis enzyme assays, we identified protein and activity for two CuZnSODs and for FeSOD and MnSOD in Arabidopsis rosette tissue. Additionally, subcellular fractionation studies revealed the presence of CSD2 and FeSOD protein within Arabidopsis chloroplasts. The seven SOD mRNAs and the four proteins identified were differentially regulated in response to various light regimes, ozone fumigation, and ultraviolet-B irradiation. To our knowledge, this is the first report of a large-scale analysis of the regulation of multiple SOD proteins in a plant species.

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Section: Map Positions Of the Known Sod Structural Genessupporting

confidence: 78%

“…To map CSD3 and the other SOD loci, PCR primers were designed that specifically amplified the SOD genes from pooled CICYAC clones in a multiplex collection of CICYAC DNA (Creusot et al, 1995). These oligonucleotides are listed in Table I.…”

Section: Map Positions Of the Known Sod Structural Genesmentioning

confidence: 99%

Superoxide Dismutase in Arabidopsis: An Eclectic Enzyme Family with Disparate Regulation and Protein Localization

1998

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“…5S rDNA units were amplified by PCR using CIC YACs as template DNA (Creusot et al 1995). The primer sequences, the positions of which are given in Figure 2B, were: dG3: GNCAAANTTNGCATGTG and dG4: CNGTNTANAAGT TATNGAGTC.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Cloix¹,

Tutois²,

Yukawa³

et al. 2001

Genome Res.

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One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5Ј-and 3Ј-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.

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“…We verified the map position of the Ds(Hyg) causing the im-2 mutation. We found that the Ds(Hyg) flanking sequence hybridizes with yeast artificial chromosomes (YACs) CIC5A4, CIC5C2, and CIC7A5 from the CIC library (Creusot et al, 1995). These YACs were mapped previously to the position of markers JBG9 on the chromosome 4 physical map (Lister and Dean, 1993;Schmidt et al, 1995) within the same area at which im has been mapped.…”

Section: Ds(hyg) Is Associated With Im-2mentioning

confidence: 99%

Mutations in the Arabidopsis Gene IMMUTANS Cause a Variegated Phenotype by Inactivating a Chloroplast Terminal Oxidase Associated with Phytoene Desaturation

et al. 1999

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The immutans ( im ) mutant of Arabidopsis shows a variegated phenotype comprising albino and green somatic sectors. We have cloned the IM gene by transposon tagging and show that even stable null alleles give rise to a variegated phenotype. The gene product has amino acid similarity to the mitochondrial alternative oxidase. We show that the IM protein is synthesized as a precursor polypeptide that is imported into chloroplasts and inserted into the thylakoid membrane. The albino sectors of im plants contain reduced levels of carotenoids and increased levels of the carotenoid precursor phytoene. The data presented here are consistent with a role for the IM protein as a cofactor for carotenoid desaturation. The suggested terminal oxidase function of IM appears to be essential to prevent photooxidative damage during early steps of chloroplast formation. We propose a model in which IM function is linked to phytoene desaturation and, possibly, to the respiratory activity of the chloroplast. INTRODUCTIONIn plant cells, plastid differentiation is intimately linked to organogenesis and is affected by both developmental regulatory mechanisms and environmental conditions. The complex mechanisms involved in the phototransformation of the plastids of dark-grown seedlings (etioplasts) to photosynthetically active plastids (chloroplasts) have been described extensively (reviewed in Mullet, 1988;Taylor, 1989;Chory and Susek, 1994). Alternatively, developing grass tissues provide a unique system in which a gradient of developmental stages of cells and plastids (proplastids to chloroplasts) is present (Mullet, 1988;Bilang and Bogorad, 1996; Inada et al., 1996).Mutants visibly impaired in chloroplast differentiation can be selected by their pale green, yellow, or albino color. The chlorophyll-deficient mutant olive of Antirrhinum has this phenotype (Hudson et al., 1993). Not all of these mutants are altered directly in pigment synthesis. Putative functions can be proposed for mutated genes based on the similarity of their coding sequences to known proteins. The Arabidopsis mutant albino3 (Sundberg et al., 1997) is impaired in chloroplast membrane biogenesis, as suggested by amino acid sequence similarity between ALBINO3 and the yeast mitochondrial OXA1 protein involved in mitochondrial biogenesis. The Arabidopsis cla1 mutation (Mandel et al., 1996) identifies an enzyme also present in cyanobacteria. Mutations also have been described that affect the proplastid-tochloroplast transition. The dag mutant from Antirrhinum (Chatterjee et al., 1996) and dcl1 from tomato (Keddie et al., 1996) do not contain chloroplasts but rather plastids resembling nondifferentiated proplastids. These genes together with the PALE CRESS gene from Arabidopsis (Reiter et al., 1994) affect both chloroplast development and leaf architecture.One class of mutations gives rise to variegated plants that have a mutant phenotype in some sectors and a wild-type phenotype in others. The iojap (Han et al., 1992) mutant of maize or the chloroplast mutator in ma...

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The CIC library: a large insert YAC library for genome mapping in Arabidopsis thaliana

Cited by 171 publications

References 0 publications

Superoxide Dismutase in Arabidopsis: An Eclectic Enzyme Family with Disparate Regulation and Protein Localization

Superoxide Dismutase in Arabidopsis: An Eclectic Enzyme Family with Disparate Regulation and Protein Localization

Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Mutations in the Arabidopsis Gene IMMUTANS Cause a Variegated Phenotype by Inactivating a Chloroplast Terminal Oxidase Associated with Phytoene Desaturation

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