The chromosome regions for increasing early growth in rice: role of sucrose biosynthesis and NH4
                     + uptake

Nagai, Yuki; Matsumoto, Kouichi; Kakinuma, Yuri; Ujiie, Kazuhiro; Ishimaru, Ken; Gamage, Dananjali; Thompson, Michael; Milham, Paul J.; Seneweera, Saman; Hirotsu, Naoki

doi:10.1007/s10681-016-1743-x

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…cDNA synthesis was carried out via reverse transcription reactions using the Primer script RT reagent kit according to the manufacturer’s instructions (Takara Bio Inc., Japan) using a total sample volume of 10 μL. Expression analysis was performed using real-time PCR 45 . All the experiments were performed using three biological replicates and the 2 -ΔΔCT method was used to calculate relative quantities 46 .…”

Section: Methodsmentioning

confidence: 99%

Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Perera

Fukushima

Akabane

et al. 2019

Sci Rep

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Phytic acid (PA) is the primary phosphorus (P) storage compound in the seeds of cereals and legumes. Low PA crops, which are considered an effective way to improve grain nutrient availability and combat environmental issues relating to seed P have been developed using mutational and reverse genetics approaches. Here, we identify molecular mechanism regulating PA content among natural rice variants. First, we performed genome-wide association (GWA) mapping of world rice core collection (WRC) accessions to understand the genetic determinants underlying PA trait in rice. Further, a comparative study was undertaken to identify the differences in PA accumulation, protein profiles, and gene expression in low (WRC 5) and high PA (WRC 6) accessions. GWA results identified myo-inositol 3-phosphate synthase 1 (INO1) as being closely localized to a significant single nucleotide polymorphism. We found high rates of PA accumulation 10 days after flowering, and our results indicate that INO1 expression was significantly higher in WRC 6 than in WRC 5. Seed proteome assays found that the expression of INO1 was significantly higher in WRC 6. These results suggest that not only the gene itself but regulation of INO1 gene expression at early developmental stages is important in determining PA content in rice.

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Section: Methodsmentioning

confidence: 99%

Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Perera

Fukushima

Akabane

et al. 2019

Sci Rep

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“…Rice was grown for 4 weeks hydroponically in a growth chamber as described by Nagai et al [38]. The shoots were cut 3-4 cm above the shoot meristem; the section was covered with silicon tube containing glass wool, and then xylem sap was collected for 2 h (10:00-12:00 h).…”

Section: Analysis Of Xylem Sapmentioning

confidence: 99%

Genotypic Differences in the Effect of P Fertilization on Phytic Acid Content in Rice Grain

Fukushima

Perera²,

Hosoya

et al. 2020

Plants

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Phytic acid (PA) prevents the absorption of minerals in the human intestine, and it is regarded as an antinutrient. Low PA rice is beneficial because of its higher Zn bioavailability and it is suggested that the gene expression level of myo-inositol 3-phosphate synthase 1 (INO1) in developing grain is a key factor to explain the genotypic difference in PA accumulation among natural variants of rice. P fertilization is also considered to affect the PA content, but it is not clear how it affects INO1 gene expression and the PA content in different genotypes. Here, we investigated the effect of P fertilization on the PA content in two contrasting rice genotypes, with low and high PA accumulation, respectively. Based on the results of the analysis of the PA content, inorganic P content, INO1 gene expression, and xylem sap inorganic P content, we concluded that the effect of P fertilization on PA accumulation in grain differed with the genotype, and it was regulated by multiple mechanisms.

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Identification and characteristics of quantitative trait locus for grain protein content, TGP12, in rice (Oryza sativa L.)

Kashiwagi

Munakata

2018

Euphytica

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The chromosome regions for increasing early growth in rice: role of sucrose biosynthesis and NH4 + uptake

Cited by 5 publications

References 29 publications

Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain

Genotypic Differences in the Effect of P Fertilization on Phytic Acid Content in Rice Grain

Identification and characteristics of quantitative trait locus for grain protein content, TGP12, in rice (Oryza sativa L.)

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