“…29). A total of 116 proteins containing both KEN box and D box were selected for further analysis (Set 1, Supplementary Table S1; (ii) copy number variation of these 2,752 genes were analyzed in our previous pooled ESCC sequencing cohort (27), with a variation frequency cutoff (larger than 20%) yielding 440 candidate genes (Set 2, Supplementary Table S2); (iii) genes overlapping of Set 1 and Set 2 were put forward for further study. Accordingly, we figured out 17 cell-cycle-related metabolic genes, including 10 CNV gain genes (ATP13A3, PIK3R4, PLCH1, TXNRD3, IDS, HS6ST2, RDH11, PLCG1, SLC12A5, and IDH3B) and 7 CNV loss genes (KCND1, AGPAT5, MOCOS, GALNT1, PDSS1, ATP10D, and LNPEP), suggesting their critical roles during ESCC progression (Fig.…”