The Chromosomal Passenger Complex Activates Polo Kinase at Centromeres

Carmena, Mar; Pinson, Xavier; Platani, Melpomeni; Salloum, Zeina; Xu, Zhenjie; Clark, Anthony; MacIsaac, Fiona; Ogawa, Hiromi; Eggert, Ulrike; Glover, David M.; Archambault, Vincent; Earnshaw, William C.

doi:10.1371/journal.pbio.1001250

Cited by 104 publications

(133 citation statements)

References 54 publications

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“…Our data cannot rule out the possibility that Aurora-B can phosphorylate a small pool of Plk1 in mitosis at specific locations or during a limited period of time. The recent work by Carmena et al indeed suggests that Aurora-B-dependent activation of Polo in Drosophila is restricted to a very short period in mitosis (Carmena et al, 2012). However, unlike Carmena et al, we found that the kinetochore signal in human cells does not disappear when Plk1 is depleted, indicating that this signal corresponds to a different epitope.…”

Section: Discussioncontrasting

confidence: 66%

“…These observations indicate that the contribution of Aurora-B to overall T210 phosphorylation of Plk1 in mitosis in human cells is very minimal. This is not in line with previous observations that Aurora-B depletion can lead to a reduction in T210-phosphorylated Plk1 at kinetochores in mitosis in human cells (Carmena et al, 2012). However, it should be noted that ab39068 was used in that study to determine the level of T210-phosphorylated Plk1 at kinetochores, which we find to recognize an epitope that might not be T210-phosphorylated Plk1 (see Fig.…”

Section: Aurora-a Regulates T210 Phosphorylation Of Plk1 In Mitosiscontrasting

confidence: 68%

“…To further characterize the spatiotemporal pattern of T210 phosphorylation, we made use of two phosphospecific antibodies raised against a phosphorylated peptide encompassing the T210 region of Plk1; ab39068 from Abcam and bd558400 from BD Biosciences (Carmena et al, 2012;Macůrek et al, 2009). To obtain mitotic cells, we subjected synchronized cultures of U2OS osteosarcoma cells to a mitotic shake-off, which resulted in samples that contained well over 90% mitotic cells (Fig.…”

Section: Analysis Of T210 Phosphorylation In Mitosismentioning

confidence: 99%

“…A logical candidate is the Aurora kinase family member Aurora-B, known to phosphorylate several proteins at kinetochores in mitosis (Ruchaud et al, 2007). Interestingly, a recent report indeed showed that Aurora-B is the kinase responsible for T-loop phosphorylation of Polo kinase at kinetochores in Drosophila and that Aurora-B could also contribute to T210 phosphorylation in human cells (Carmena et al, 2012). Therefore, we investigated the possibility of sequential activation of Plk1 by Aurora-A and Aurora-B.…”

Section: Aurora-a Regulates T210 Phosphorylation Of Plk1 In Mitosismentioning

confidence: 99%

“…In yeast, Cdk1 has been shown to activate Cdc5 (the sole Polo homolog in budding yeast) through phosphorylation of a T-loop residue corresponding to human T214 (Mortensen et al, 2005). However, it has recently been shown that another member of the aurora kinase family, Aurora-B can phosphorylate Polo in Drosophila and can also contribute to T210 phosphorylation during mitosis in mammalian cells (Carmena et al, 2012). Aurora-B is part of the chromosomal passenger complex and has several roles during mitosis, including error correction of microtubule attachments at the kinetochores and cytokinesis (van der Waal et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Bora and Aurora-A continue to activate Plk1 in mitosis

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Section: Discussioncontrasting

confidence: 66%

Section: Aurora-a Regulates T210 Phosphorylation Of Plk1 In Mitosiscontrasting

confidence: 68%

Section: Analysis Of T210 Phosphorylation In Mitosismentioning

confidence: 99%

Section: Aurora-a Regulates T210 Phosphorylation Of Plk1 In Mitosismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bora and Aurora-A continue to activate Plk1 in mitosis

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et al. 2013

Journal of Cell Science

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Deciphering the spatio‐temporal regulation of entry and progression through mitosis

Gheghiani

Gavet

2014

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Mitosis has been studied since the early 1880s as a key event of the cell division cycle where remarkable changes in cellular architecture take place and ultimately lead to an equal segregation of duplicated chromosomes into two daughter cells. A detailed description of the complex and highly ordered cellular events taking place is now available. Many regulators involved in key steps including entry into mitosis, nuclear envelope breakdown, microtubule (MT) spindle formation, and chromosome attachment, as well as mitotic exit and cytokinesis, have also been identified. However, understanding the precise spatio-temporal contribution of each regulator in the cell reorganization process has been technically challenging. This review will focus on a number of recent advances in our understanding of the spatial distribution of protein activities and the temporal regulation of their activation and inactivation during entry and progression through mitosis by the use of intramolecular Förster resonance energy transfer (FRET)-based biosensors.

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Centriole is the pivot co‐ordinating dynamic signaling for cell proliferation and organization during early development in the vertebrates

Roopasree

Adivitiya

Chakraborty

et al. 2021

Cell Biology International

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Vertebrates have an elaborate and functionally segmented body. It evolves from a single cell by systematic cell proliferation but attains a complex body structure with exquisite precision. This development requires two cellular events: cell cycle and ciliogenesis. For these events, the dynamic molecular signaling is converged at the centriole. The cell cycle helps in cell proliferation and growth of the body and is a highly regulated and integrated process. Its errors cause malignancies and developmental disorders. The cells newly proliferated are organized during organogenesis. For a cellular organization, dedicated signaling hubs are developed in the cells, and most often cilia are utilized. The cilium is generated from one of the centrioles involved in cell proliferation. The developmental signaling pathways hosted in cilia are essential for the elaboration of the body plan. The cilium's compartmental seclusion is ideal for noise‐free molecular signaling and is essential for the precision of the body layout. The dysfunctional centrioles and primary cilia distort the development of body layout that manifest as serious developmental disorders. Thus, centriole has a dual role in the growth and cellular organization. It organizes dynamically expressed molecules of cell cycle and ciliogenesis and plays a balancing act to generate new cells and organize them during development. A putative master molecule may regulate and co‐ordinate the dynamic gene expression at the centrioles. The convergence of many critical signaling components at the centriole reiterates the idea that centriole is a major molecular workstation involved in elaborating the structural design and complexity in vertebrates.

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The Chromosomal Passenger Complex Activates Polo Kinase at Centromeres

Abstract: INCENP acts as a protein scaffold that integrates the functions of two crucial mitotic kinases, Aurora B and Polo, at centromeres of mitotic chromosomes.

Cited by 104 publications

References 54 publications

Bora and Aurora-A continue to activate Plk1 in mitosis

Bora and Aurora-A continue to activate Plk1 in mitosis

Deciphering the spatio‐temporal regulation of entry and progression through mitosis

Centriole is the pivot co‐ordinating dynamic signaling for cell proliferation and organization during early development in the vertebrates

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