The chromosomal constitution of fish hybrid lineage revealed by 5S rDNA FISH

Zhang, Chun; Ye, Lihai; Chen, Yiyi; Xiao, Jun; Wu, Yanhong; Tao, Min; Xiao, Yamei; Liu, Shaojun

doi:10.1186/s12863-015-0295-8

Cited by 26 publications

(21 citation statements)

References 30 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first of these fragments has been sequenced and published in the GenBank (Zhu, Ma, & Gui, 2006). The latter 5S rDNA gene class, with an approximate length of 200 bp shown in C. gibelio in the present study, has also been reported in C. auratus, but the authors did not provide any discussion involving this rDNA fragment (He et al, 2012, Zhang et al, 2015.…”

Section: Discussionmentioning

confidence: 58%

Untitled

Szabelska¹,

Kirtiklis²,

Przybył³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The Prussian carp may occur as diploid (2n=100) and/or triploid (3n=150) individuals, co-existing in many natural populations. The simultaneous occurrence of individuals with different ploidy makes the taxonomy of this species unclear. Additionally, the taxonomic status of C. gibelio has become even more enigmatic due to its hybridizing with other nonindigenous cyprinids. Since the variation within 5S rDNA can serve as a suitable marker for molecular identification of the fish ploidy, the main aim of present study was to compare this rDNA sequence between Prussian carp individuals with different ploidy levels: diploids (2n =100) and triploids (3n=150-160). PCR amplification of 5S rDNA generated two bands of approximately 340 and 470 bp in length (in both diploids and triploids) and band 200 bp visible in some individuals. These results indicate the presence of at least two different classes of 5S rRNA gene. Analysis of their nucleotide composition revealed no differences within the class of 340 bp and several nucleotide differences within the class of 470 bp between diploid and triploid individuals. The 5S rDNA variability detected in this study indicates the potential usefulness of this sequence for the identification of diploid and triploid individuals of the Prussian carp.

show abstract

Section: Discussionmentioning

confidence: 58%

Untitled

Szabelska¹,

Kirtiklis²,

Przybył³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

“…Chromosomal mapping of rDNA clusters has repeatedly helped to unveil diverse evolutionary issues (e.g., [82,83]). Particularly in fishes, it provided valuable clues about the incidence of cryptic, morphologically indistinguishable sibling species [5,6,8,10,84], polyploidization and interspecific hybridization events [85,86], a geographical gradient of genomic and morphological change [87], patterns of sex chromosome differentiation [80,[88][89][90], and the correlation of genome dynamics in response to environmental cues [91,92]. Among the Nannostomus species investigated here, chromosomal mapping revealed somewhat uniform patterns of distribution for both rDNA classes, with one to few sites of accumulation, as found in most fishes [93,94], as well as in some other lebiasinids [21,22] investigated to date.…”

Section: Discussionmentioning

confidence: 99%

Centric Fusions behind the Karyotype Evolution of Neotropical Nannostomus Pencilfishes (Characiforme, Lebiasinidae): First Insights from a Molecular Cytogenetic Perspective

Sember

Oliveira

Ráb

et al. 2020

Genes

View full text Add to dashboard Cite

Lebiasinidae is a Neotropical freshwater family widely distributed throughout South and Central America. Due to their often very small body size, Lebiasinidae species are cytogenetically challenging and hence largely underexplored. However, the available but limited karyotype data already suggested a high interspecific variability in the diploid chromosome number (2n), which is pronounced in the speciose genus Nannostomus, a popular taxon in ornamental fish trade due to its remarkable body coloration. Aiming to more deeply examine the karyotype diversification in Nannostomus, we combined conventional cytogenetics (Giemsa-staining and C-banding) with the chromosomal mapping of tandemly repeated 5S and 18S rDNA clusters and with interspecific comparative genomic hybridization (CGH) to investigate genomes of four representative Nannostomus species: N. beckfordi, N. eques, N. marginatus, and N. unifasciatus. Our data showed a remarkable variability in 2n, ranging from 2n = 22 in N. unifasciatus (karyotype composed exclusively of metacentrics/submetacentrics) to 2n = 44 in N. beckfordi (karyotype composed entirely of acrocentrics). On the other hand, patterns of 18S and 5S rDNA distribution in the analyzed karyotypes remained rather conservative, with only two 18S and two to four 5S rDNA sites. In view of the mostly unchanged number of chromosome arms (FN = 44) in all but one species (N. eques; FN = 36), and with respect to the current phylogenetic hypothesis, we propose Robertsonian translocations to be a significant contributor to the karyotype differentiation in (at least herein studied) Nannostomus species. Interspecific comparative genome hybridization (CGH) using whole genomic DNAs mapped against the chromosome background of N. beckfordi found a moderate divergence in the repetitive DNA content among the species’ genomes. Collectively, our data suggest that the karyotype differentiation in Nannostomus has been largely driven by major structural rearrangements, accompanied by only low to moderate dynamics of repetitive DNA at the sub-chromosomal level. Possible mechanisms and factors behind the elevated tolerance to such a rate of karyotype change in Nannostomus are discussed.

show abstract

“…Chromosome preparations were carried out as described previously [18]. FISH was performed according to the method described by Masaru and Hideo with minor modifications [17].…”

Section: Methodsmentioning

confidence: 99%

Variations in 5S rDNAs in diploid and tetraploid offspring of red crucian carp × common carp

Chun

Tang

et al. 2017

BMC Genet

Self Cite

View full text Add to dashboard Cite

BackgroundThe allotetraploid hybrid fish (4nAT) that was created in a previous study through an intergeneric cross between red crucian carp (Carassius auratus red var., ♀) and common carp (Cyprinus carpio L., ♂) provided an excellent platform to investigate the effect of hybridization and polyploidization on the evolution of 5S rDNA. The 5S rDNAs of paternal common carp were made up of a coding sequence (CDS) and a non-transcribed spacer (NTS) unit, and while the 5S rDNAs of maternal red crucian carp contained a CDS and a NTS unit, they also contained a variable number of interposed regions (IPRs). The CDSs of the 5S rDNAs in both parental fishes were conserved, while their NTS units seemed to have been subjected to rapid evolution.ResultsThe diploid hybrid 2nF1 inherited all the types of 5S rDNAs in both progenitors and there were no signs of homeologous recombination in the 5S rDNAs of 2nF1 by sequencing of PCR products. We obtained two segments of 5S rDNA with a total length of 16,457 bp from allotetraploid offspring 4nAT through bacterial artificial chromosome (BAC) sequencing. Using this sequence together with the 5S rDNA sequences amplified from the genomic DNA of 4nAT, we deduced that the 5S rDNAs of 4nAT might be inherited from the maternal progenitor red crucian carp. Additionally, the IPRs in the 5S rDNAs of 4nAT contained A-repeats and TA-repeats, which was not the case for the IPRs in the 5S rDNAs of 2nF1. We also detected two signals of a 200-bp fragment of 5S rDNA in the chromosomes of parental progenitors and hybrid progenies by fluorescence in situ hybridization (FISH).ConclusionsWe deduced that during the evolution of 5S rDNAs in different ploidy hybrid fishes, interlocus gene conversion events and tandem repeat insertion events might occurred in the process of polyploidization. This study provided new insights into the relationship among the evolution of 5S rDNAs, hybridization and polyploidization, which were significant in clarifying the genome evolution of polyploid fish.

show abstract

The chromosomal constitution of fish hybrid lineage revealed by 5S rDNA FISH

Cited by 26 publications

References 30 publications

Untitled

Untitled

Centric Fusions behind the Karyotype Evolution of Neotropical Nannostomus Pencilfishes (Characiforme, Lebiasinidae): First Insights from a Molecular Cytogenetic Perspective

Variations in 5S rDNAs in diploid and tetraploid offspring of red crucian carp × common carp

Contact Info

Product

Resources

About