The chlorophyll‐protein complexes of the thylakoid in greening plastids of <i>Phaseolus vulgaris</i>

Argyroudi‐Akoyunoglou, J. H.; Akoyunoglou, George

doi:10.1016/0014-5793(79)81088-1

Cited by 92 publications

(39 citation statements)

References 19 publications

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“…Chloroplasts and thylakoids were prepared from Phaseolus vulgaris leaves as in [7]. The thylakoids, washed twice in 0.05 M Tricine-NaOH (pH 7.3) were solubilized in 0.3 M Tris-HC1 (pH 8.6)-10% glycerol-l% SDS to have ~700 #g chl/ml, SDS/chl = 6-7, and 0.4-0.5% SDS.…”

Section: Methodsmentioning

confidence: 99%

“…Absorption spectra were recorded on a Perkin Elmer 356 double beam spectrophotometer and low temperature fluorescence spectra in a fluorometer setup described in [17]. SDS-PAGE was done as in [7,18]. The eiectrophoretic profiles were recorded in a JoyceLoeble Chromoscan, using a cut-off filter transmitting light above 620 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Earlier methods resolved 2 complexes: CPI, the chl a-rich PT00-protein complex, and CPII or LHCP, the chl a + b-rich light-harvesting complex [1,2]. Milder methods resolve 6 main complexes and some minor bands, with >90% of the thylakoid chlorophyl bound to them: the monomers CPI and LHCP3, their oligomers CPIa, and LHCP1 and LHCP2, respectively, and a minor chl a containing complex (CPa, complex IV, A, or chl a-P2-chl a-P3) [3][4][5][6][7][8], thought to originate in the PSII reaction center. The oligomers increase during greening [9] and are transformed to their monomers by extensive SDS action or cation neutralization of the electrostatic forces, which seem to prevail [ 10].…”

Section: Introductionmentioning

confidence: 99%

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Separation of thylakoid pigment—protein complexes by SDS—sucrose density gradient centrifugation

Argyroudi‐Akoyunoglou

Thomou

1981

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Separation of thylakoid pigment—protein complexes by SDS—sucrose density gradient centrifugation

Argyroudi‐Akoyunoglou

Thomou

1981

FEBS Letters

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“…The first relates to anomalies created by the use of the electrophoretic techniques on samples which were argued to be uniform (9). The second school of thought is that there is variability in the basic subunit structure of the thylakoids which is under environmental control (1). Hitherto, no one has considered the possibility of such anomalies being generated by l Supported in part by the Swiss National Science Foundation (Grant numbers 3.119.77 and 3.661.080 to P. A. S.), the Emil-Barrel Foundation and the European Molecular Biological Organization (postdoctoral fellowship to L. E. A. H.).…”

Section: Photosystemsmentioning

confidence: 99%

Alteration in the Acyl Lipid Composition of Thylakoids Induced by Aging and Its Effect on Thylakoid Structure

Henry

Strasser²,

Siegenthaler³

1982

Plant Physiol.

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The alteration in both the lipid composition and chlorophyll proteins obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of detergent solubilized thylakoids was investigated on differentially aged spinach (Spinacia oleracea L.) chloroplasts. Freshly isolated material demonstrated seven major bands upon electrophoretic fractionation. Membranes aged in vitro showed a diminution and/or a disappearance of some bands concomitant with changes in the acyl lipid composition of these membranes. The extent of these changes was influenced by the purity of the preparation. Low temperature fluorescence measurements (77K) showed that upon aging, the photochemical capacity of photosystem II decreased prior to alterations in the molecular organization of the photochemical apparatus as indicated by the energy distribution between the two photosystems.The results are discussed in terms of whether the series of changes in the acyl lipid composition upon aging is related to the variations in the electrophoretic pattern of the chlorophyll proteins.Extensive efforts have been made to elucidate the polypeptide composition of thylakoids and to determine the spatial arrangements of the individual components within the membrane. In this respect, selective solubilization of the thylakoids by the use of detergents and subsequent examinations of the resulting pigmentcontaining fractions, using electrophoretic techniques, has been particularly useful. Numerous pigmented bands have been identified (see review [23]). However, analyses using the above mentioned approach in various laboratories have given both quantitative and qualitative differences in the individual components resolved (23).There are two main schools of thought for rationalizing the above observations. The first relates to anomalies created by the use of the electrophoretic techniques on samples which were argued to be uniform (9). The second school of thought is that there is variability in the basic subunit structure of the thylakoids which is under environmental control (1). Hitherto, no one has

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“…The rate of Chl accumulation in this case is high and parallels that of the other thylakoid components. In plants exposed to intermittent light, however, Chl is bound selectively on RC proteins and only Abbreviations: PS, photosystem; LHC, light-harvesting complex; Chl, chlorophyll; CPI, P700-Chl a protein complex of PS I; CPIa, PSI Chl-protein complex containing CPI and LHC-I; CAP, chloramphenicol; CL, continuous light; LDC, light-dark cycles; ImL, intermittent light; D, dark; cyt., cytochrome RC core complexes are stabilized in thylakoids [1,2]. Since no LHCs can be detected in the membrane, in spite of the fact that the mRNA for the LHC-II apoprotein is present and can be translated [3], it has been proposed that LHC stabilization is controlled at the post-translational level [4,5]: whenever the rate of Chl accumulation is reduced, while polypeptide synthesis remains unaffected, there is a competition between polypeptides for the small amount of Chl available.…”

Section: Introductionmentioning

confidence: 99%

Chloramphenicol‐induced stabilization of light‐harvesting complexes in thylakoids during development

Tzinas

Argyroudi‐Akoyunoglou

1988

FEBS Letters

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Chloramphenicol treatment allows light‐harvesting apoprotein accumulation in thylakoids of intermittent light plants and prevents its digestion in plants transferred to darkness after brief pre‐exposure to continuous light. This suggests that under conditions where reaction center‐core protein synthesis is inhibited, the light‐harvesting apoproteins can be stabilized. The results support the competition hypothesis proposed to occur between reaction center polypeptides and light‐harvesting apoproteins for chlorophyll, whenever the rate of chlorophyll formation relative to that of the polypeptide components is limited.

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The chlorophyll‐protein complexes of the thylakoid in greening plastids of Phaseolus vulgaris

Cited by 92 publications

References 19 publications

Separation of thylakoid pigment—protein complexes by SDS—sucrose density gradient centrifugation

Separation of thylakoid pigment—protein complexes by SDS—sucrose density gradient centrifugation

Alteration in the Acyl Lipid Composition of Thylakoids Induced by Aging and Its Effect on Thylakoid Structure

Chloramphenicol‐induced stabilization of light‐harvesting complexes in thylakoids during development

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