The Chlamydomonas Flagellum as a Model for Human Ciliary Disease

Pazour, Gregory J.; Witman, George B.

doi:10.1016/b978-0-12-370873-1.00052-6

Cited by 16 publications

(17 citation statements)

References 132 publications

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“…In particular, the cilia of Chlamydomonas are highly similar to those in mammals, making this alga an excellent system for studying ciliary disease [14, 15]. Because the cilia of Chlamydomonas are not essential mutants unable to assemble flagella can be isolated and studied, making this system uniquely useful.…”

Section: Chlamydomonas – a Reference Green Algamentioning

confidence: 99%

The Chlamydomonas genome project: a decade on

Blaby

Blaby-Haas

Tourasse

et al. 2014

Trends in Plant Science

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The green alga Chlamydomonas reinhardtii is a popular unicellular organism for studying photosynthesis, cilia biogenesis and micronutrient homeostasis. Ten years since its genome project was initiated, an iterative process of improvements to the genome and gene predictions has propelled this organism to the forefront of the “omics” era. Housed at Phytozome, the Joint Genome Institute’s (JGI) plant genomics portal, the most up-to-date genomic data include a genome arranged on chromosomes and high-quality gene models with alternative splice forms supported by an abundance of RNA-Seq data. Here, we present the past, present and future of Chlamydomonas genomics. Specifically, we detail progress on genome assembly and gene model refinement, discuss resources for gene annotations, functional predictions and locus ID mapping between versions and, importantly, outline a standardized framework for naming genes.

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Section: Chlamydomonas – a Reference Green Algamentioning

confidence: 99%

The Chlamydomonas genome project: a decade on

Blaby

Blaby-Haas

Tourasse

et al. 2014

Trends in Plant Science

Self Cite

145

128

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“…Eukaryotic cilia and flagella are conserved organelles required for motile and sensory functions vital for development and the function of most organs (Satir and Christensen, 2007 ). Failure in assembly or regulation of cilia results in a wide range of human diseases called “ciliopathies” (Badano et al , 2006 ; Fliegauf et al , 2007 ; Marshall, 2008 ; Pazour and Witman, 2008 ; Gerdes et al , 2009 ; Leigh et al , 2009 ; Nigg and Raff, 2009 ), yet our understanding of the assembly and mechanism of cilia is incomplete. Here we focus on the motile ciliary/flagellar axoneme and the mechanism and functional interactions of the dynein motors that power motility (Kamiya, 2002 ; Oiwa and Sakakibara, 2005 ; King and Kamiya, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Distinct roles of 1α and 1β heavy chains of the inner arm dynein I1 ofChlamydomonasflagella

Toba

Fox

Sakakibara

et al. 2011

MBoC

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“…Biofilms are important under a wide range of conditions, whether in natural environment or in infectious diseases [ 33 ]. Detachment from a glass surface of the adhered microalgae flagella is interesting because it seems to involve signals that govern the activity of the motor apparatus, flagellar activity, and the multiple functions in the regulation of flagellar beating, which can be linked to human ciliary activity studies [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Detachment of Dunaliella tertiolecta Microalgae from a Glass Surface by a Near-Infrared Optical Trap

Juarez

Garces²,

Cordero-Esquivel

et al. 2020

Sensors

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We report on the observation of the detachment in situ and in vivo of Dunaliella tertiolecta microalgae cells from a glass surface using a 1064 nm wavelength trapping laser beam. The principal bends of both flagella of Dunaliella were seen self-adhered to either the top or bottom coverslip surfaces of a 50 μm thick chamber. When a selected attached Dunaliella was placed in the trapping site, it photoresponded to the laser beam by moving its body and flagellar tips, which eventually resulted in its detachment. The dependence of the time required for detachment on the trapping power was measured. No significant difference was found in the detachment time for cells detached from the top or bottom coverslip, indicating that the induced detachment was not due solely to the optical forces applied to the cells. After detachment, the cells remained within the optical trap. Dunaliella detached from the bottom were seen rotating about their long axis in a counterclockwise direction, while those detached from the top did not rotate. The rotation frequency and the minimal force required to escape from the trap were also measured. The average rotation frequency was found to be independent of the trapping power, and the swimming force of a cell escaping the laser trap ranged from 4 to 10 picoNewtons. Our observations provide insight into the photostimulus produced when a near-infrared trapping beam encounters a Dunaliella. The microalgae frequently absorb more light than they can actually use in photosynthesis, which could cause genetic and molecular changes. Our findings may open new research directions into the study of photomovement in species of Dunaliella and other swimming microorganisms that could eventually help to solve technological problems currently confronting biomass production. In future work, studies of the response to excess light may uncover unrecognized mechanisms of photoprotection and photoacclimation.

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The Chlamydomonas Flagellum as a Model for Human Ciliary Disease

Cited by 16 publications

References 132 publications

The Chlamydomonas genome project: a decade on

The Chlamydomonas genome project: a decade on

Distinct roles of 1α and 1β heavy chains of the inner arm dynein I1 ofChlamydomonasflagella

Detachment of Dunaliella tertiolecta Microalgae from a Glass Surface by a Near-Infrared Optical Trap

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