The chlamydia: molecular biology of procaryotic obligate parasites of eucaryocytes.

Becker, Y

doi:10.1128/mmbr.42.2.274-306.1978

Cited by 46 publications

(30 citation statements)

References 81 publications

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“…By applying a polycationic ferritin derivative as an ultrastructural probe, a dense and homogeneous layer of anionic sites could be visualized on the outer membrane surfaces of all developmental forms of both C. psittaci and C. trachomatis. This result was quite unexpected since it indicates that chemical groups which significantly contribute to the chlamydial surface architecture, are kept constant in spite of the well-known morphological and biochemical alterations [1][2][3] of the composition and organization of the cell membranes during the chlamydial life cycle.…”

Section: Discussionmentioning

confidence: 96%

“…Chlamydiae are obligate intracellular microorganisms with a typical developmental life cycle [1][2][3]: infectious, extracellular, metabolically inert elementary bodies enter the cytoplasm of a eukaryotic host cell where they differentiate through a dispersing form into non-infectious, metabolically active reticulate bodies which, within the phagocytic vacuole, multiply by binary fission, and further reorganize through a condensing form to elementary bodies which are finally released by cell rupture.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

“…The chlamydiae possess a distinct trilaminar outer membrane and a second more narrow and less distinct trilaminar cytoplasmic membrane. Significant changes in the composition and organization of the cell membranes occur during the chlamydial life cycle [1][2][3].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

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Anionic sites on Chlamydia membranes

Schiefer

1982

FEMS Microbiology Letters

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Section: Discussionmentioning

confidence: 96%

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

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Anionic sites on Chlamydia membranes

Schiefer

1982

FEMS Microbiology Letters

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“…Members of the genus Chlamydia are obligate intracellular parasites with a unique growth cycle [1]. After attachment to the surface of susceptible cells, the bacteria are internalized into host cells by a process sensitive to inhibitors of glycolysis [2] and oxidative phosphorylation [3], but resistant to cytochalasin B [4].…”

Section: Mccoy Cells To Infection With Chlamydia Trachomatismentioning

confidence: 99%

“…Ingested chlamydiae are sequestered into intracellular vesicles and initially no fusion with lysosomes occurs [3,5]. When elementary bodies have entered cells they reorganize into reticulate bodies are metabolically active, synthesizing macromolecules and multiplying by binary fission until about 20 h. Then they undergo another reorganization and mature into new elementary bodies which eventually are released from the infected cell [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Trifluoperazine inhibits the infectivity of Chlamydia trachomatis for McCoy cells

Kihlström

1983

FEMS Microbiology Letters

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Trifluoperazine (TFP), an inhibitor of the CaZ+-binding protein calmodulin, was used to study the infectivity of Chlamydia trachomatis for McCoy cells. TFP inhibited the number of chlamydial inclusions and the chlamydia-dependent amino acid incorporation when added within 9 h after inoculation with chlamydiae. However, TFP did not affect the attachment of chlamydiae to the cells or the protease-removable fraction of cellbound chlamydiae.These results suggest that an early step in the intracellular development of chlamydiae, partly coinciding with the elementary body-reticulate body conversion, is sensitive to TFP and that clathrin coats are not crucial in the ingestion of chlamydiae by McCoy cells.

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Nucleic acid analysis by sandwich hybridization

Nicholls

Malcolm

1989

Clinical Laboratory Analysis

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P e t e r J o h n N i c h o l l s . A T h e s i s S u b m it t e d f o r t h e D e g r e e o f D o c t o r o f P h i lo s o p h y o f t h e U n i v e r s i t y o f L o n d o n . C h a r i n g C r o s s a n d W e s t m in s t e r M e d i c a l S c h o o l.M a r c h , 1 9 8 9 . 2 A b s t r a c t . T h e n u c l e i c a c i d -b a s e d t e s t s c u r r e n t l y u s e d f o r t h e d e t e c t io n o f i n h e r i t e d a n d i n f e c t i o u s d i s e a s e a r e s e n s i t i v e a n d s p e c i f i c , b u t n o t a lw a y s r e p r o d u c ib le , b e c a u s e o f p r o c e d u r a l c o m p l e x i t i e s ; t h i s i n t u r n m eans t h a t t h e y a r e n o t a m e n a b le t o a u t o m a t io n . S u c h t e s t s a r e n o t c o s t -e f f e c t i v e , a r e l i m i t e d i n t h e num ber o f s a m p le s w h ic h c a n b e p r o c e s s e d a t o n c e , a n d m o s t com m only r e l y o n u n s t a b le i s o t o p i c a l l y l a b e l l e d p r o b e s , w h ic h a r e h a z a r d o u s t o h a n d le a n d d i s p o s e o f . A n u c l e i c a c i d s a n d w ic h h y b r i d i z a t i o n a s s a y h a s b e e n d e v e lo p e d , i n i t i a l l y u s i n g c lo n e d hum an p a p i l l o m a v i r u s (H PV) s e q u e n c e s a s a m o d e l, a n d s u b s e q u e n t ly f o r t h e d e t e c t io n a n d t y p i n g o f H PV i n f e c t i o n i n c e r v i c a l s c r a p e s . T h e i m p l i c a t i o n s o f c e r v i c a l HPV i n f e c t i o n a r e o f c l i n i c a l s i g n i f i c a n c e , b e c a u s e o f t h e r e l a t i v e l y r e c e n t a c c u m u la t io n o f e v id e n c e l i n k i n g s p e c i f i c H PV t y p e s w i t h t h e a e t i o l o g y o f c e r v i c a l c a n c e r . C o n s i d e r i n g t h a t v i r a l t y p e c o u ld h a v e a g r e a t i n f l u e n c e o n t h e p r o g n o s i s o f i n f e c t i o n , a n d t h a t im m u n o lo g ic a l a s s a y s a r e n o t c a p a b leo f d i f f e r e n t i a t i n g b e tw e e n t y p e s , t h e s a n d w ic h h y b r i d i z a t i o n t e c h n iq u e , w h ic h c o u ld h a n d le l a r g e n um bers o f s a m p le s , w o u ld b e a n i n v a l u a b l e t o o l f o r im p r o v in g t h e e f f i c a c y o f t h e c e r v i c a l s c r e e n i n g p rogram m e. T o Mun a n d D ad, w ith a l l n y lo v e . T h e a s s a y r e q u i r e s tw o d i s t i n c t , n o n -o v e r la p p i n g ENA fr a g m e n t s , d e r iv e d fr o m a d j a c e n t p o s i t i o n s o n t h e v i r a l genom e. One f r a g m e n t (A ) i s c o v a l e n t l y a t t a c h e d t o a S-T h a n k s f o r m a k in g t h i s p o s s i b l e - 5T h e kncw n i s f i n i t e , t h e unknow n i n f i n i t e ; i n t e l l e c t u a l l y we s t a n d o n a n i s l e t i n t h e m id s t o f a n i l l i m i t a b l e o c e a n o f i n e x p l i c a b i l i t y . O u r b u s i n e s s i n e v e r y g e n e r a t io n i s t o r e c l a i m a l i t t l e m ore la n d .T .H . H u x le y , 1 8 8 7 . F i r s t a n d f o r e m o s t ,...

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The chlamydia: molecular biology of procaryotic obligate parasites of eucaryocytes.

Cited by 46 publications

References 81 publications

Anionic sites on Chlamydia membranes

Anionic sites on Chlamydia membranes

Trifluoperazine inhibits the infectivity of Chlamydia trachomatis for McCoy cells

Nucleic acid analysis by sandwich hybridization

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