The Chinese or Striped-Back Hamster

Feeney, William P.

doi:10.1016/b978-0-12-380920-9.00035-3

Cited by 4 publications

(2 citation statements)

References 113 publications

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“…Cricetulus griseus or Chinese hamster was first used as a laboratory animal to type pneumococci obtained from human patients 11 and became a valuable animal model in other infectious disease and epidemiology research studies 12 , including MERS-CoV, SARS-CoV 13 and SARS-CoV-2 14 . There is also a Chinese hamster ovary (CHO) cell line which is widely used in biological and medical research, primarily for toxicity screening and for expression of recombinant antibodies.…”

Section: Discussionmentioning

confidence: 99%

Host genomes for the unique SARS-CoV-2 variant leaked into Antarctic soil metagenomic sequencing data

Csabai

Solymosi

2022

Preprint

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Recently Csabai et al.1 have found a most likely contaminated metagenomic sample set from Antarctica that contained traces of unique SARS-CoV-2 variants. This is a short followup of that report where we attempt to find genetic footprint of the hosts. With reasonable confidence we could identify genetic material from mitochondria of Homo sapiens, green monkey and Chinese hamster. The latter two most probably originated from cell lines Vero E6 (or COS-7) and CHO respectively, which are frequent laboratory culture media for studying viruses including SARS-CoV-2 and its closest relatives.

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Section: Discussionmentioning

confidence: 99%

Host genomes for the unique SARS-CoV-2 variant leaked into Antarctic soil metagenomic sequencing data

Csabai

Solymosi

2022

Preprint

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“…Chinese hamster ovary (CHO) cell lines have become the most prominent host cell system for recombinant protein production, producing over 60% of all recombinant protein therapeutics produced today . CHO cells are also among the most important cell lines used in biomedical research for decades (reviewed in ). Their small number of chromosomes and relative ease of deriving mutant lines made these cell lines a valuable cytogenetic tool .…”

Section: Introductionmentioning

confidence: 99%

Augmenting Chinese hamster genome assembly by identifying regions of high confidence

Vishwanathan

Bandyopadhyay

et al. 2016

Biotechnology Journal

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Chinese hamster Ovary (CHO) cell lines are the dominant industrial workhorses for therapeutic recombinant protein production. The availability of genome sequence of Chinese hamster and CHO cells will spur further genome and RNA sequencing of producing cell lines. However, the mammalian genomes assembled using shot-gun sequencing data still contain regions of uncertain quality due to assembly errors. Identifying high confidence regions in the assembled genome will facilitate its use for cell engineering and genome engineering. We assembled two independent drafts of Chinese hamster genome by de novo assembly from shotgun sequencing reads and by re-scaffolding and gap-filling the draft genome from NCBI for improved scaffold lengths and gap fractions. We then used the two independent assemblies to identify high confidence regions using two different approaches. First, the two independent assemblies were compared at the sequence level to identify their consensus regions as "high confidence regions" which accounts for at least 78 % of the assembled genome. Further, a genome wide comparison of the Chinese hamster scaffolds with mouse chromosomes revealed scaffolds with large blocks of collinearity, which were also compiled as high-quality scaffolds. Genome scale collinearity was complemented with EST based synteny which also revealed conserved gene order compared to mouse. As cell line sequencing becomes more commonly practiced, the approaches reported here are useful for assessing the quality of assembly and potentially facilitate the engineering of cell lines.

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The Laboratory Hamster

Tambare

Murkunde

2021

Essentials of Laboratory Animal Science: Principles and Practices

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The Chinese or Striped-Back Hamster

Cited by 4 publications

References 113 publications

Host genomes for the unique SARS-CoV-2 variant leaked into Antarctic soil metagenomic sequencing data

Host genomes for the unique SARS-CoV-2 variant leaked into Antarctic soil metagenomic sequencing data

Augmenting Chinese hamster genome assembly by identifying regions of high confidence

The Laboratory Hamster

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