The chicken Pdcd4 gene is regulated by v-Myb

Schlichter, Ursula; Burk, Oliver; Worpenberg, Susanne; Klempnauer, Karl‐Heinz

doi:10.1038/sj.onc.1204071

Cited by 51 publications

(51 citation statements)

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“…Homologs in mice (MA-3/TIS) (4,5), chickens (6,7) and rats (DUG) (8) have also been reported. The expression of the gene is modulated by many factors, including interleukins (9), retinoid (10), 12-O-tetradecanoylphorbol 13 acetate (TPA) (11,12), transforming growth factor (TGF)-β1 (13), growth factors (14,15), topoisomerase inhibitors (5) and the transcription factor myb (6,7), and is up-regulated in association with apoptosis (4,13). PDCD4 expression has been shown to be suppressed in many tumor tissues, such as lung cancers (16), pancreatic cancers (17), hepatocellular carcinomas (13), colon cancers (18), skin carcinomas (15), breast cancers (19) and glioma tissues (20).…”

Section: Introductionmentioning

confidence: 92%

Expression patterns of the tumor suppressor PDCD4 and correlation with β-catenin expression in gastric cancers

Kakimoto

Shiraishi²,

Iwakiri³

et al. 2011

Oncol Rep

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Abstract. The expression patterns of PDCD4, a tumor suppressor, and β-catenin were immunohistologically investigated in gastric carcinoma tissues. In normal gastric tissues, PDCD4 was strongly expressed in the cell nuclei, but weakly expressed in the cytoplasm. In gastric adenocarcinoma tissues, nuclear PDCD4 expression was decreased, while cytoplasmic PDCD4 expression was unchanged or somewhat increased. In gastric signet ring cell carcinoma tissues, PDCD4 expression patterns were different from the expression patterns of the adenocarcinoma tissues, and PDCD4 was localized in the nuclei of the carcinoma cells as a belt in the middle of the epithelial layer. The nuclear localization of PDCD4 in the adenocarcinoma tissues was correlated with the membrane localization of β-catenin, the activation of which stimulates invasion of colon cancer cells. PDCD4 expression was correlated with β-catenin expression in gastric carcinoma cell lines, but not with E-cadherin, as the binding partner in the cell membrane.

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Section: Introductionmentioning

confidence: 92%

Expression patterns of the tumor suppressor PDCD4 and correlation with β-catenin expression in gastric cancers

Kakimoto

Shiraishi²,

Iwakiri³

et al. 2011

Oncol Rep

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“…pCMVluc was obtained by inserting the CMV promoter into pGL3-basic (Promega, Mannheim, Germany). Expression vectors for hemagglutinin-tagged chicken Pdcd4 and wild-type human Pdcd4 have been described (Schlichter et al, 2001a;Waters et al, 2007) pCDNA4-hPdcd4-mutRBM1 þ 2 encodes a mutant Pdcd4 protein with amino-acid substitutions of several basic amino acids in the N-terminal part of Pdcd4 (Wedeken et al, 2010). Bacterial expression vectors for GST-Pdcd4 and GST-Pdcd4 mutRBM1 þ 2 have been described (Wedeken et al, 2010).…”

Section: Expression Vectorsmentioning

confidence: 99%

Pdcd4 directly binds the coding region of c-myb mRNA and suppresses its translation

et al. 2011

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Pdcd4 is a novel tumor suppressor protein that functions in the nucleus and the cytoplasm, and appears to be involved in the regulation of transcription and translation. In the cytoplasm, Pdcd4 has been implicated in the suppression of translation of mRNAs containing structured 5 0 -untranslated regions; however, the mechanisms that recruit Pdcd4 to specific target mRNAs and the identities of these mRNAs are mostly unknown. In this study, we have identified c-myb mRNA as the first natural translational target mRNA of Pdcd4. We have found that translational suppression of c-myb mRNA by Pdcd4 is dependent on sequences located within the c-myb-coding region. Furthermore, we have found that the N-terminal domain of Pdcd4 has an important role in targeting Pdcd4 to c-myb RNA by mediating preferential RNA binding to the Pdcd4-responsive region of c-myb mRNA. Overall, our work demonstrates for the first time that Pdcd4 is directly involved in translational suppression of a natural mRNA and provides the first evidence for a key role of the RNA-binding domain in targeting Pdcd4 to a specific mRNA.

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“…The human gene is located at chromosome 10q24 (Soejima et al, 1999) and its expression has been reported, for example, in human interleukin 12 (IL-12)-induced natural killer (NK) and T cells (Azzoni et al, 1998), small duct epithelial cells of the normal mammary gland (Soejima et al, 1999;Yoshinaga et al, 1999), a growth-suppressed human neuroendocrine pancreatic carcinoma cells (Stalberg et al, 2001), normal human lung tissue (Chen et al, 2003), or senescent human fibroblasts (Kang et al, 2002). Pdcd4 has been suggested to be linked to the process of apoptosis in response to different inducers (Shibahara et al, 1995;Zhang and DuBois, 2001), and has been shown to be regulated by topoisomerase inhibitors (Onishi et al, 1998), COX-2 inhibitors (Zhang and DuBois, 2001), Myb (Schlichter et al, 2001) and Akt (Palamarchuk et al, 2005). Molecules regulated by Pdcd4 include p21 (Go¨ke et al, 2004), Cdk4, ornithine decarboxylase (Jansen et al, 2005), carbonic anhydrase II (LankatButtgereit et al, 2004) and JNK/c-Jun/AP-1 (Bitomsky et al, 2004;Yang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Tumor suppressor Pdcd4 inhibits invasion/intravasation and regulates urokinase receptor (u-PAR) gene expression via Sp-transcription factors

et al. 2007

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The chicken Pdcd4 gene is regulated by v-Myb

Cited by 51 publications

References 50 publications

Expression patterns of the tumor suppressor PDCD4 and correlation with β-catenin expression in gastric cancers

Expression patterns of the tumor suppressor PDCD4 and correlation with β-catenin expression in gastric cancers

Pdcd4 directly binds the coding region of c-myb mRNA and suppresses its translation

Tumor suppressor Pdcd4 inhibits invasion/intravasation and regulates urokinase receptor (u-PAR) gene expression via Sp-transcription factors

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