The chicken blastoderm: current views on cell biological events guiding intercellular communication

Harrisson, F.; Andries, Luc; Vakaet, L

doi:10.1016/0045-6039(88)90021-8

Cited by 19 publications

(8 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of extensive gap junctions (Revel et al, 1973) and the detection of the gene transcript for the ion channel protein connexin43 (Yancey et al, 1992) strongly suggest that pathways for cell-cell communication are now fully established in the embryonic ectoderm. In the chick blastoderm, there is a similar increase in cell junctions as gastrulation proceeds (Harrison et al, 1988).…”

Section: Initiation Of Gastrulationmentioning

confidence: 91%

Gastrulation in the mouse embryo: Ultrastructural and molecular aspects of germ layer morphogenesis

Tam¹,

Williams²,

Chan³

1993

Microscopy Res & Technique

View full text Add to dashboard Cite

Ultrastructural studies and lineage analyses of gastrulating mouse embryos have revealed that different morphogenetic tissue movements are involved in the formation of the three definitive germ layers. Definitive ectoderm is formed by epibolic expansion of the pre-existing progenitor population in the embryonic ectoderm. Formation of the mesoderm and the endoderm is initiated by cellular ingression at the primitive streak. The mesodermal layer is established by cell migration and cell sheet spreading, but the endoderm is formed by replacing the original primitive endodermal population. To this date, genes that are expressed during mouse gastrulation mostly encode cell surface adhesion or signalling molecules, growth factors and their receptors, and putative transcriptional factors. Their precise role during gastrulation remains to be investigated.

show abstract

Section: Initiation Of Gastrulationmentioning

confidence: 91%

Gastrulation in the mouse embryo: Ultrastructural and molecular aspects of germ layer morphogenesis

Tam¹,

Williams²,

Chan³

1993

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…Fertilized chicken eggs (Warren SSL isabrown strain) from a commercial stock were incubated at 38°C for 4 to 24 h, to obtain stage-1 to stage-9 blastoderms (Vakaet, 1970;Harrisson et al, 1988a). Unincubated and incubated blastoderms were explanted on a glass ring according to the technique of New (1955), and fixed for 1 h at room temperature in a solution containing 1% (wh) glutaraldehyde and 0.5% (wh) tannic acid (mol.…”

Section: Tissue Preparation For Electron Microscopymentioning

confidence: 99%

Features of polyingression and primitive streak ingression through the basal lamina in the chicken blastoderm

1991

Self Cite

View full text Add to dashboard Cite

The de-epithelialization of cells of the upper layer during the phenomena of polyingression and primitive streak ingression was studied by analyzing, from the time of laying to the end of gastrulation, the ultrastructure of the basal lamina underlying the upper layer. The electron density of the basal lamina and associated extracellular materials was enhanced by addition of tannic acid to the fixative. Special attention was also paid to the spatial and temporal distribution of blebs at the basal surface of the upper layer, and to the contribution of the de-epithelialized cells to the formation of the deep layer. The results indicate that a nascent basal lamina is already present at the time of laying, especially beneath regions of the area pellucida where polyingression is not apparent. From the onset of incubation, the basal lamina rapidly develops, and it is interrupted by a large number of blebs. However, during the first 6-8 h of incubation, i.e., stages 1-2 of Vakaet (Arch. Biol. (Liège) 81:387-426, 1970), a downward movement of de-epithelialized cells that insert into the deep layer and form the endophyll persists cranially. This phenomenon of polyingression, which starts during the intrauterine period, probably extends from caudal to cranial and comes to an end by stage 3. During these first three stages, the number of blebs progressively decreases, especially in the cranial part of the area pellucida, and a thicker, continuous basal lamina associated with numerous interstitial bodies is laid down. The caudal part of the upper layer is still actively blebbing at that time. Due to the convergence of this area toward the axis of the blastoderm, which leads to ingression at and elongation of the primitive streak up to and including stage 6, the number of blebs at the basal surface of the upper layer progressively decreases. From stage 7 on, blebs are virtually absent; shortening of the primitive streak and formation of the head process begin. At the level of the head process, primitive streak ingression has ceased and a novel basal lamina is progressively deposited beneath the upper layer. By stage 9, a thick, smooth basal lamina physically separates the upper layer from the head mesenchyme. Summarizing, at the time of gastrulation, the presence of blebs that perforate the basal lamina is correlated with the de-epithelialization of cells. Before incubation, however, de-epithelialization of upper-layer cells occurs before the assembly of the basal lamina.(ABSTRACT TRUNCATED AT 400 WORDS)

show abstract

“…18). DISCUSSION Development of primary endoderm in the domestic fowl, as revealed by SEM, involves somewhat more complex growth patterns and origins than those customarily described by individual investigators (reviewed by Romanoff, 1961;Harrisson et al, 1988). The three zones of the ventral surface of the area pellucida recognizable during the first 6 hours of incubation (Fig.…”

Section: Resultsmentioning

confidence: 93%

“…The many factors involved in early development in the chick are described in the review by Harrisson et al (1988). Among these are striking morphological differences, described by SEM, that exist between the dor-sal and ventral surfaces of embryos up to 4 days incubation age.…”

mentioning

confidence: 97%

See 1 more Smart Citation

Scanning electron microscopy of developing primary endoderm during first 6 hours of incubation in the chick

Low¹,

McClugage²

1993

Anat. Rec.

View full text Add to dashboard Cite

Development of primary endoderm in the domestic fowl (Gallus domesticus) is described in scanning electron microscopy (SEM) supplemented by transmission electron microscopy (TEM). Although complicated by great variability, the ventral surface of the blastoderm reveals this process during the first 6 hours of incubation. Primary endoderm arises (1) from the hypoblast, (2) from the margin of the area pellucida, and (3) from intervening portions of the area pellucida. The early hypoblast becomes several cells thick while individual cells are still spherical. TEM reveals a variety of immature cell junctions. During subsequent flattening of these cells into primary endodermal epithelium, numerous filopodia arise from their surfaces. These are 0.20-0.25 microns in diameter. They become long and branched, attaching to each other and to other cell bodies. Similar filopodial processes are present less conspicuously among cells in the margin of the area pellucida. Here, there is pseudopodial evidence that cells or cell sheets creep along the ventral surface of the epiblast. The filopodia disappear as cell flattening proceeds. The ventral surface of the exposed epiblast delaminates cells that become free after their exploratory filopodia and lamellipodia are put forth. Lateral contacts among cell bodies from the above three sources increase until a continuous epithelium is formed. The primary endoderm of the embryo, a simple squamous epithelium that separates the connective tissue space above from the gastrocoele below, is generated by these developmental events.

show abstract

The chicken blastoderm: current views on cell biological events guiding intercellular communication

Cited by 19 publications

References 90 publications

Gastrulation in the mouse embryo: Ultrastructural and molecular aspects of germ layer morphogenesis

Gastrulation in the mouse embryo: Ultrastructural and molecular aspects of germ layer morphogenesis

Features of polyingression and primitive streak ingression through the basal lamina in the chicken blastoderm

Scanning electron microscopy of developing primary endoderm during first 6 hours of incubation in the chick

Contact Info

Product

Resources

About