The chick embryo chorioallantoic membrane as an in vivo experimental model to study human neuroblastoma

Ribatti, Doménico; Tamma, Roberto

doi:10.1002/jcp.26773

Cited by 34 publications

(26 citation statements)

References 33 publications

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“…Additionally, CAM is an embryonic tissue lacking an immune system and having mesenchymal cells with stem like potential able to differentiate depending on a specific microenvironment [15,16]. Based on the previously described features, CAM is a reliable in vivo model for testing the behavior of normal and pathologic tissues (as cultured cells or malignant tumors) [17,18], of different drugs and antibodies [19,20], or of a variety of biomaterials implanted on its surface [21,22]. There is minimal data available regarding bone implants on CAM [23,24].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

Cirligeriu

Cîmpean

Călniceanu

et al. 2018

IJMS

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Microscopic and molecular events related to alveolar ridge augmentation are less known because of the lack of experimental models and limited molecular markers used to evaluate this process. We propose here the chick embryo chorioallantoic membrane (CAM) as an in vivo model to study the interaction between CAM and bone substitutes (B) combined with hyaluronic acid (BH), saline solution (BHS and BS, respectively), or both, aiming to point out the microscopic and molecular events assessed by Runt-related transcription factor 2 (RUNX 2), osteonectin (SPARC), and Bone Morphogenic Protein 4 (BMP4). The BH complex induced osteoprogenitor and osteoblastic differentiation of CAM mesenchymal cells, certified by the RUNX2 +, BMP4 +, and SPARC + phenotypes capable of bone matrix synthesis and mineralization. A strong angiogenic response without inflammation was detected on microscopic specimens of the BH combination compared with an inflammatory induced angiogenesis for the BS and BHS combinations. A multilayered organization of the BH complex grafted on CAM was detected with a differential expression of RUNX2, BMP4, and SPARC. The BH complex induced CAM mesenchymal cells differentiation through osteoblastic lineage with a sustained angiogenic response not related with inflammation. Thus, bone granules resuspended in hyaluronic acid seem to be the best combination for a proper non-inflammatory response in alveolar ridge augmentation. The CAM model allows us to assess the early events of the bone substitutes–mesenchymal cells interaction related to osteoblastic differentiation, an important step in alveolar ridge augmentation.

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Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

Cirligeriu

Cîmpean

Călniceanu

et al. 2018

IJMS

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“…The CAM has a rich vascular system and acts as an organ for gas-exchange during chick embryonic development. The CAM has been exploited as a relatively simple, time-and cost-efficient model to study various cancer related processes including angiogenesis, cancer cell invasion, metastasis formation and tumor progression [10][11][12][13] . One important aspect of this in vivo system is that the chick embryo is immunodeficient up until E18, just prior to hatching 14 , affording the possibility to transplant donor cells and tissue without an adverse host response.…”

mentioning

confidence: 99%

CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells

Palaniappan

Šlekienė

Jonasson

et al. 2020

Sci Rep

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the development of metastases is the major cause of cancer related death. to develop a standardized method that define the ability of human cancer cells to degrade the basement membrane, e.g. the delamination capacity, is of importance to assess metastatic aggressiveness. We now present the in vivo CAM-Delam assay to visualize and quantify the ability of human cancer cells to delaminate and invade. The method includes seeding cancer cells on the chick chorioallantoic membrane (CAM), followed by the evaluation of cancer-induced delamination and potential invasion within hours to a few days. By testing a range of human cancer cell lines in the cAM-Delam assay, our results show that the delamination capacity can be divided into four categories and used to quantify metastatic aggressiveness. our results emphasize the usefulness of this assay for quantifying delamination capacity as a measurement of metastatic aggressiveness, and in unraveling the molecular mechanisms that regulate delamination, invasion, formation of micro-metastases and modulations of the tumor microenvironment. This method will be useful in both the preclinical and clinical characterization of tumor biopsies, and in the validation of compounds that may improve survival in metastatic cancer.

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“…The CAM assay is a frequently applied model to study cancer metastasis [39][40][41][42] . However, there are only a few studies that have used CAM assays to assess Nbl cell invasion and metastasis [43][44][45] . The UKF-NB-4 CDDP cell line displayed high resistance to CDDP and showed higher intravasation and metastasis in liver, lung, and brain than the parental cell line.…”

Section: Discussionmentioning

confidence: 99%

Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma

Rodrigo

Michalkova

Strmiska

et al. 2021

Sci Rep

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Metallothionein-3 has poorly characterized functions in neuroblastoma. Cisplatin-based chemotherapy is a major regimen to treat neuroblastoma, but its clinical efficacy is limited by chemoresistance. We investigated the impact of human metallothionein-3 (hMT3) up-regulation in neuroblastoma cells and the mechanisms underlying the cisplatin-resistance. We confirmed the cisplatin-metallothionein complex formation using mass spectrometry. Overexpression of hMT3 decreased the sensitivity of neuroblastoma UKF-NB-4 cells to cisplatin. We report, for the first time, cisplatin-sensitive human UKF-NB-4 cells remodelled into cisplatin-resistant cells via high and constitutive hMT3 expression in an in vivo model using chick chorioallantoic membrane assay. Comparative proteomic analysis demonstrated that several biological pathways related to apoptosis, transport, proteasome, and cellular stress were involved in cisplatin-resistance in hMT3 overexpressing UKF-NB-4 cells. Overall, our data confirmed that up-regulation of hMT3 positively correlated with increased cisplatin-chemoresistance in neuroblastoma, and a high level of hMT3 could be one of the causes of frequent tumour relapses.

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The chick embryo chorioallantoic membrane as an in vivo experimental model to study human neuroblastoma

Cited by 34 publications

References 33 publications

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

CAM-Delam: an in vivo approach to visualize and quantify the delamination and invasion capacity of human cancer cells

Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma

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