The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b

Porra, Robert J.

doi:10.1007/1-4020-3324-9_56

Cited by 344 publications

(416 citation statements)

References 27 publications

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“…Biweekly, the following parameters were measured: the number of runners (stolons), leaf number, fruit number, fruit fresh weight, yield, fruit size, marketability (score 1-4; 1 -extra quality; 2 -good quality; 3 -medium quality; 4 -non marketable); total soluble solids (TSS); titratable acidity (TA); total phenolics (as described previously in Tzortzakis et al 2007). Leaf chlorophyll (chl a and chl b) content was determined according to Porra (2002) while leaf fluoresces, photosynthetic rate (P n ), stomatal conductance (g s ) and internal leaf concentration of CO 2 (C i ) were determined using a portable infra-red gas analyser (model Li-6200; Li-Cor, Inc., Lincoln, USA). Measurements were carried out between 9:00-11:10 AM, the leaf temperature within the chamber was 28 ± 2°C, photosynthetic photon flux density of 1300 μmol/m 2 /s was used at the ambient CO 2 concentration.…”

Section: Methodsmentioning

confidence: 99%

Use of sawdust, coco soil and pumice in hydroponically grown strawberry

Marinou¹,

Chrysargyris²,

Tzortzakis³

2013

Plant Soil Environ.

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Strawberry (Fragaria × ananassa Duch.) plants were grown hydroponically in different ratio of sawdust (Saw-100), coco soil (Coc-100) and/or pumice (Pum-100) mixtures. Leaf number doubled in plants grown in Saw-100 while runners (stolons) number increased up to 70% in plants grown in Coc-100 compared with the control treatment (Pum-100). Fruit number increased (up to 50%) in plants grown in Pum-100. Leaf stomatal conductance, photosynthetic rate and internal concentration of CO 2 differentiated according to the plant vegetative or reproductive stage and/or substrate medium. Leaf and stem fresh weight as well as leaf area was increased (up to 32, 24 and 44%, respectively) in case of Coc-100 compared with the Saw-100 or Pum-100. Plant yield was doubled when Pum-Saw (50-50) was used compared with the Saw-100, which is due to the reduced fruit number produced rather than the difference in fruit fresh weight. Substrate affected fruit quality parameters. The present findings highlight the putative use of organic medium i.e. Sawdust on top of the widely used coco soil as substrate medium in strawberry culture.

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Section: Methodsmentioning

confidence: 99%

Use of sawdust, coco soil and pumice in hydroponically grown strawberry

Marinou¹,

Chrysargyris²,

Tzortzakis³

2013

Plant Soil Environ.

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“…The absorbance at 663 and 645 nm were measured and total chlorophyll contents were calculated as described elsewhere (Porra 2002).…”

Section: Chlorophyll Measurementsmentioning

confidence: 99%

The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress

et al. 2015

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“…Chlorophyll was extracted from powdered samples with 80 % acetone, and chlorophyll concentration was determined by measuring absorption at 663 and 646 nm [39].…”

Section: Measurement Of Chlorophyll Concentrationmentioning

confidence: 99%

PvNAC1 and PvNAC2 Are Associated with Leaf Senescence and Nitrogen Use Efficiency in Switchgrass

et al. 2014

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Two full-length cDNAs encoding NAM, ATAF, and CUC (NAC)-family transcription factors (TFs) were isolated from two different cultivars of Panicum virgatum L. (switchgrass) and named PvNAC1 and PvNAC2. Phylogenetic analysis of PvNAC1 and PvNAC2 grouped them with NAC proteins involved in senescence in annual plant species. Transcript profiling revealed that both PvNAC1 and PvNAC2 are induced during leaf senescence. Expression of a PvNAC1-green fluorescent protein (GFP) fusion in plant cells revealed a nuclear location of the protein, consistent with a role in transcriptional regulation. Expression of PvNAC1 in an Arabidopsis nap stay-green mutant suppressed its senescence defect. Expression of PvNAC1 in wild-type Arabidopsis triggered early leaf senescence and remobilization. Transcriptome analysis implicated leaf protein degradation and nitrogen recycling enzymes in NAC-dependent seed protein increase in Arabidopsis. Overexpression of pvNAC2 in switchgrass resulted in increased aboveground biomass associated with increased transcript levels of key nitrogen metabolism genes in leaves and nitrate and ammonium transporter genes in roots. The results indicate that NAC TFs play conserved roles in leaf senescence in the plant kingdom not only in annual monocots and dicots but also in perennial plants such as switchgrass. PvNAC1 and PvNAC2 hold promise for improving nutrient use efficiency in switchgrass through genetic manipulation.

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The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b

Cited by 344 publications

References 27 publications

Use of sawdust, coco soil and pumice in hydroponically grown strawberry

Use of sawdust, coco soil and pumice in hydroponically grown strawberry

The rice transcription factor OsWRKY47 is a positive regulator of the response to water deficit stress

PvNAC1 and PvNAC2 Are Associated with Leaf Senescence and Nitrogen Use Efficiency in Switchgrass

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