Abstract:Samples of de Tomasi's Schiff reagent were allowed to age. During this process physical, chemical and staining properties of the reagent were monitored. Certain physical and chemical properties of Schiff's reagent correlated with staining intensities. Thus a fall in the iodine titre and a rise in the pH (indicating loss of sulphur dioxide) accompanied falls of intensity of Feulgen-stained frog red-cell nuclei and of P.A.S.-stained cellulose sheet. Ageing sulphite solutions exhibited analogous physical and chem… Show more
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