The chemical modification of nucleic acids

Kochetkov, Nikolay K.; Budowsky, E.I.; Demushkin, V. P.; Turchinsky, M.F.; Simukova, N.A.

doi:10.1016/0005-2787(67)90513-8

Cited by 18 publications

(6 citation statements)

References 17 publications

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“…As we have shown earlier, an increase in Na + concentration strengthens the negative shift of melting temperature as medium is changed from neutral to alkaline because of a strong decrease in pK values of nucleotide deprotonation with [Na + ] [53,72]. That fact also supports the proposed electrostatic mechanism of additional destabilization of platinated DNA in alkaline medium.…”

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofsupporting

confidence: 76%

“…Thus, alkaline medium makes DNA thermal destabilization common to all considered platinum compounds. On the other hand, DNA is chemically stable at pH 10.5 [53]. That allows the use of alkaline medium in various DNA studies and in methods of isolation of plasmid DNA.…”

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofmentioning

confidence: 99%

“…That allows the use of alkaline medium in various DNA studies and in methods of isolation of plasmid DNA. Such stability is not inherent to RNA in alkaline medium as well as for DNA in acidic medium [53].…”

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofmentioning

confidence: 99%

“…DNA deprotonation causes a destabilizing effect at pH >10, i.e., when pH is higher than pK's of nitrogen bases (see [53,72] for detail). In alkaline medium, thermal destabilization takes place for both unmodified and platinated DNA because the majority protons able to dissociate from DNA in alkaline medium are included in the formation hydrogen bonds that generate the double helix [53,72]. Binding of platinum compounds decreases DNA charge density in helical regions adjacent to destroyed sites of platination.…”

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofmentioning

confidence: 99%

“…In contrast to RNA, DNA is chemically stable in alkaline medium [53]. Therefore alkaline medium is used in DNA studies, purification and isolation methods.…”

mentioning

confidence: 99%

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Temporal behavior of DNA thermal stability in the presence of platinum compounds. Role of monofunctional and bifunctional adducts

Lando¹,

Galyuk²,

Chang³

et al. 2012

Journal of Inorganic Biochemistry

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Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofsupporting

confidence: 76%

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofmentioning

confidence: 99%

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofmentioning

confidence: 99%

Section: The Mechanism Of a Stronger Decrease In Thermal Stability Ofmentioning

confidence: 99%

“…In contrast to RNA, DNA is chemically stable in alkaline medium [53]. Therefore alkaline medium is used in DNA studies, purification and isolation methods.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Temporal behavior of DNA thermal stability in the presence of platinum compounds. Role of monofunctional and bifunctional adducts

Lando¹,

Galyuk²,

Chang³

et al. 2012

Journal of Inorganic Biochemistry

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Sequencing Strategies and Tactics inDNAandRNAAnalysis

Azhikina

2000

Encyclopedia of Analytical Chemistry

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Sequencing procedures are aimed at the determination of sequences of monomer units constituting linear biopolymer molecules. In the particular case of nucleic acids the monomer units are DNA or RNA residues. The present article reviews several sequencing strategies: different approaches (random or systematic sequencing) as well as their combinations giving the best results for long‐range sequencing applicable to long and unique DNA fragments, including entire genomes, up to the Homo sapiens genome size; massive sequencing of relatively short fragments such as RNAs, complementary DNAs (cDNAs), etc.; and comparative sequencing of related DNA fragments originating from various individuals or different species. Although particular sequencing approaches may involve different ways of DNA treatment (chemical, enzymatic, or physical), they are all based on the same idea: a labeled molecule is cut to obtain a set of fragments all having one common end, other ends being different owing to the statistical character of the nucleic acid chain cleavage. The fragments obtained are then separated according to their lengths. The primary structure of nucleic acids is a key not only to physical and chemical properties of the molecules but also to the biological (genetic) information therein, including structures of genes and proteins, the ways of gene regulation and cell response to different signals. This is a great advantage over other physical, chemical, or biological methods of DNA/RNA analysis. However, sequencing in itself is usually insufficient to predict unambiguously the biological properties and functions of the encoded products.

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Sequencing Strategies and Tactics inDNAandRNAAnalysis

Azhikina

2014

Encyclopedia of Analytical Chemistry

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The primary structure of nucleic acids is a key not only to physical and chemical properties of the molecules but also to the biological (genetic) information therein, including structures of genes and proteins, the ways of gene regulation and cell response to different signals. Sequencing procedures are aimed at the determination of sequences of monomer units constituting linear biopolymer molecules, DNA or RNA residues in case of nucleic acids. This article reviews existing sequencing strategies: different approaches of random or systematic sequencing applicable to long and unique DNA fragments, including entire genomes; massive sequencing of relatively short fragments such as RNAs and complementary DNAs (cDNAs); and comparative sequencing of related DNA fragments originating from various individuals or different species.

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The chemical modification of nucleic acids

Cited by 18 publications

References 17 publications

Temporal behavior of DNA thermal stability in the presence of platinum compounds. Role of monofunctional and bifunctional adducts

Temporal behavior of DNA thermal stability in the presence of platinum compounds. Role of monofunctional and bifunctional adducts

Sequencing Strategies and Tactics inDNAandRNAAnalysis

Sequencing Strategies and Tactics inDNAandRNAAnalysis

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