The chemical ecology of the fly

Mansourian, Suzan; Stensmyr, Marcus C.

doi:10.1016/j.conb.2015.02.006

Cited by 89 publications

(98 citation statements)

References 59 publications

(89 reference statements)

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“…Seven ORNs showed no significant population differences in their response properties ( figure 1a,b). This high level of conservation in five of the seven ORNs is in line with our understanding from D. melanogaster that they function as general detectors of habitat suitability, for instance, as a general yeast detector (ab1A, ab2A, ab7B, pb2B and pb3B [28,38,39]). The receptors of the remaining two ORNs are unknown.…”

Section: (B) Population Differences In Electrophysiological Responsessupporting

confidence: 79%

“…Having established this within D. mojavensis, we used the mean responses of these subtypes in a cluster analysis with recordings of known D. melanogaster subtypes. This reveals that the three large antennal basiconic (ab) subtypes (Dmojab1 -3) were highly similar in their response properties to their D. melanogaster equivalent (figure 1a; electronic supplementary material, figure S2, [9,18,26,28] Other ORNs did not, and these responses fell into two categories: (i) ORNs previously shown to have high variability among drosophilid species [28,35,38,39] or (ii) ORNs in which the corresponding receptor is not bioinformatically predicted to be present in D. mojavensis [35]. The remaining three, DmojabX -Z, could not be unambiguously matched by their responses to known D. melanogaster subtypes.…”

Section: Results (A) Characterization Of the Response Properties Of Dmentioning

confidence: 93%

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Population differences in olfaction accompany host shift in Drosophila mojavensis

et al. 2016

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Evolutionary shifts in plant -herbivore interactions provide a model for understanding the link among the evolution of behaviour, ecological specialization and incipient speciation. Drosophila mojavensis uses different host cacti across its range, and volatile chemicals emitted by the host are the primary cue for host plant identification. In this study, we show that changes in host plant use between distinct D. mojavensis populations are accompanied by changes in the olfactory system. Specifically, we observe differences in olfactory receptor neuron specificity and sensitivity, as well as changes in sensillar subtype abundance, between populations. Additionally, RNA-seq analyses reveal differential gene expression between populations for members of the odorant receptor gene family. Hence, alterations in host preference are associated with changes in development, regulation and function at the olfactory periphery.

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Section: (B) Population Differences In Electrophysiological Responsessupporting

confidence: 79%

Section: Results (A) Characterization Of the Response Properties Of Dmentioning

confidence: 93%

Population differences in olfaction accompany host shift in Drosophila mojavensis

et al. 2016

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“…1A), stimulating them with 100 µmol l −1 solutions of the general AC agonist forskolin, the phosphodiesterase (PDE) inhibitor IBMX, the OR agonist VUAA1, an odor mixture composed of ethyl acetate and methyl acetate (two acetate esters produced by yeasts that are highly attractive to flies; Mansourian and Stensmyr, 2015) or, as a control, a solution containing 0.1% DMSO. In all cases except the control, we could detect a strong signal with a characteristic time course (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…food odors (Mansourian and Stensmyr, 2015 ] i ) affects the activity of some isoforms of the eukaryotic class III ACs (Linder, 2006), stimuli consisting of 100 µmol l −1 ethyl hexanoate, VUAA1 or forskolin solutions were delivered in regular Ringer solution (containing both Ca 2+ and Na + ) or in Ca 2+ /Na + -free Ringer solution in order to prevent extracellular /Na + -free Ringer solution (E,F), or expressing a mutated Or22a (Or22a…”

Section: Resultsmentioning

confidence: 99%

“…Faint concentrations of odorant blends or even single compounds can inform insects, like the fruit fly Drosophila melanogaster, about the presence of, for example, food sources, perils or sexual partners (Hansson and Stensmyr, 2011;Mansourian and Stensmyr, 2015). The two main olfactory organs of Drosophila -the antennae and the maxillary palps -express three major classes of chemoreceptors: the odorant receptors (ORs), the gustatory receptors (GRs) and the ionotropic receptors (IRs), which are related to ionotropic glutamate receptors (Touhara and Vosshall, 2009;Joseph and Carlson, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Odor induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Miazzi

Hansson

Wicher

2016

Journal of Experimental Biology

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Insect odorant receptors are seven transmembrane domain proteins that form cation channels, whose functional properties such as receptor sensitivity are subject to regulation by intracellular signaling cascades. Here, we used the cAMP fluorescent indicator Epac1-camps to investigate the occurrence of odor-induced cAMP production in olfactory sensory neurons (OSNs) of Drosophila melanogaster. We show that stimulation of the receptor complex with an odor mixture or with the synthetic agonist VUAA1 induces a cAMP response. Moreover, we show that while the intracellular Ca 2+ concentration influences cAMP production, the OSN-specific receptor OrX is necessary to elicit cAMP responses in Ca 2+ -free conditions. These results provide direct evidence of a relationship between odorant receptor stimulation and cAMP production in olfactory sensory neurons in the fruit fly antenna and show that this method can be used to further investigate the role that this second messenger plays in insect olfaction.

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Sensory deficiencies affect resource selection and associational effects at two spatial scales

Verschut

Inouye

Hambäck

2018

Ecology and Evolution

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Many insect species have limited sensory abilities and may not be able to perceive the quality of different resource types while approaching patchily distributed resources. These restrictions may lead to differences in selection rates between separate patches and between different resource types within a patch, which may have consequences for associational effects between resources. In this study, we used an oviposition assay containing different frequencies of apple and banana substrates divided over two patches to compare resource selection rates of wild‐type Drosophila melanogaster at the between‐ and within‐patch scales. Next, we compared the wild‐type behavior with that of the olfactory‐deficient strain Orco 2 and the gustatory‐deficient strain Poxn ΔM22‐B5 and found comparable responses to patch heterogeneity and similarly strong selection rates for apple at both scales for the wild‐type and olfactory‐deficient flies. Their oviposition behavior translated into associational susceptibility for apple and associational resistance for banana. The gustatory‐deficient flies, on the other hand, no longer had a strong selection rate for apple, strongly differed in between‐ and within‐patch selection rates from the wild‐type flies, and caused no associational effects between the resources. Our study suggests that differences in sensory capabilities can affect resource selection at different search behavior scales in different ways and in turn underlie associational effects between resources at different spatial scales.

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The chemical ecology of the fly

Cited by 89 publications

References 59 publications

Population differences in olfaction accompany host shift in Drosophila mojavensis

Population differences in olfaction accompany host shift in Drosophila mojavensis

Odor induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Sensory deficiencies affect resource selection and associational effects at two spatial scales

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