The Chemical Compositions of Essential Oils Derived from Cryptocarya alba and Laurelia sempervirens Possess Antioxidant, Antibacterial and Antitumoral Activity Potential

Abstract

Cryptocarya alba (Peumo; CA) and Laurelia sempervirens (Laurel; LS) are herbs native to the Chilean highlands and have historically been used for medicinal purposes by the Huilliches people. In this work, the essential oils were extracted using hydrodistillation in Clevenger apparatus and analyzed by GC-MS to determine their composition. The antioxidant capacity (AC) was evaluated in vitro. The cytotoxicity was determined using cell line cultures both non tumoral and tumoral. The toxicity was determined using the nematode Caenorhabditis elegans. The antimicrobial activity was evaluated against 52 bacteria using the agar disc diffusion method and the minimum inhibitory concentrations (MICs) were determined. The principal compounds found in C. alba essential oil (CA_EO) were α-terpineol (24.96%) and eucalyptol (21.63%) and were isazafrol (91.9%) in L. sempervirens essential oil (LS_EO). Both EOs showed antioxidant capacity in vitro. Both EO showed antibacterial activity against bacteria using. LS_EO showed more inhibitory effect on these cell lines respect to CA_EO. Both EOs showed toxicity against the nematode C.elegans at 3.12–50 mg/mL. The essential oils of CA and LS have an important bioactive potential in their antioxidant, antibacterial and cytotoxicity activity. Both essential oils could possibly be used in the field of natural medicine, natural food preservation, cosmetics, sanitation and plaguicides among others.

Chemical Analysis and In Vitro Bioactivity of Essential Oil of Laurelia sempervirens and Safrole Derivatives against Oomycete Fish Pathogens

“…In this context, the variability in the essential oil content is influenced by the Some studies of leaf EOs from L. sempervirens showed different chemical compositions according to the region. The results of this work show that the chemical composition of this EO was different with those obtained from plants collected in the Metropolitan, Ñuble, Bio-Bío and La Araucanía regions [14,16,17,22]. The most important differences are found in the absence of spatulenol content, the lower isosafrole content, and a higher safrole content.…”

“…is an evergreen endemic tree of the temperate rainforests of southern Chile [13]. Numerous pharmacological and phytochemical studies have reported repellent, insecticidal, antibacterial, antioxidant, antitumoral and antifungal activity potential of essential oil from L. sempervirens [14][15][16][17].…”

Essential Oils of Three Balkan Abies Species: Chemical Profiles, Antimicrobial Activity and Toxicity toward Artemia salina and Drosophila melanogaster

“…Studies on the antimicrobial potential of camphene demonstrated a broad spectrum but variable activity of this compound, depending on the strain and technique used for antimicrobial testing [25] . Limonene was previously reported to possess moderate to strong antimicrobial activity, [26–28] but it was also determined that this activity may be enhanced by acting synergistically with other compounds, so in mixtures such as EOs the activity of both limonene and accompanying compounds is higher [29] . In the case of the herein tested EOs, we cannot relate the activity of the EOs to presence of these two compounds.…”

Antimicrobial Properties of Chilean Native Plants: Future Aspects in Their Application in the Food Industry

“…The fruit is edible (cooked or fresh), and the aromatic leaves can be used as a facial mask to clean the skin [ 33 ]. In addition, the EOs of this specie seem to have antioxidant and antibacterial properties [ 45 ].…”