The characterization, renoprotection and antioxidation of enzymatic and acidic exopolysaccharides from Hypsizigus marmoreus

Abstract

The present work was designed to investigate the characterization, as well as the antioxidation and renoprotection in streptozocin (STZ)-induced diabetic mice, of exopolysaccharides (EPS) and the enzymatic-EPS (EEPS) and acidic-EPS (AEPS) hydrolysates, which were separated from the fermentation liquor of Hypsizigus marmoreus. Animal results demonstrated that EPS, EEPS and AEPS had potential antioxidant and renoprotective effects, especially EEPS. Additionally, they were the most effective, reflecting increases in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), total antioxidant capacity (T-AOC), and albumin (ALB) of 168.33%, 124.8%, 268.17% 179.49%, and 68.71%, respectively, and decreases in the contents of malondialdehyde (MDA), lipid peroxide (LPO) and levels of serum urea nitrogen (BUN) and creatinine (CRE) by 70.58%, 58.43%, 23.97% and 29.60%, respectively, at a dose of 800 mg/kg compared to those of model mice. Three polysaccharides ameliorated the histopathological alterations which were observed in the kidney of diabetic mice. Furthermore, the characterization of polysaccharides had been expressed. These findings indicated that the EEPS from H. marmoreus possesses more effective renoprotection and antioxidation effects and provided insight into its potential clinical values on preventing diabetes.

Beneficial Effect of Intestinal Fermentation of Natural Polysaccharides

“…Exopolysaccharides separated from the fermentation liquor of Hypsizigus marmoreus ameliorated the histopathological alterations in the kidney of streptozocin-induced diabetic mice. Additionally, an increase in superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, total antioxidant capacity, and albumin, and a decrease in the contents of malondialdehyde, lipid peroxide and levels of serum urea nitrogen and creatinine were observed [ 135 ]. Additionally, the ratio of Firmicutes / Bacteroidetes and the richness of Ruminococcaceae and Lactobacilli increased to achieve antidiabetogenic effect.…”

Chemical characterization of Tianshan green tea polysaccharides and its protective effects on cell oxidative injury

“…SOD is the main antioxidant enzyme in the body, which can scavenge superoxide anion (Chen & Li, 2007). CAT is an enzyme that converts hydrogen peroxide, which is able to protect cells from the H 2 O 2 -induced injury (Liu, Lan et al, 2018).…”

Bioreactor-grown exo- and endo-β-glucan from Malaysian Ganoderma lucidum: An in vitro and in vivo study for potential antidiabetic treatment

“…Few studies have previously shown the inhibition activities of EPS toward the glucosidase enzyme. However, the EPS from previous studies were extracted mostly from plants and bacteria ( Priatni et al, 2016 ; Sasikumar et al, 2017 ), and there is scarce literature that shows mushroom EPS and to the best of our knowledge, none of ENS as alpha-glucosidase inhibitors ( Liu et al, 2018 ).…”