The characterization of structural variations within a crystal field

Single-Particle Approaches in the Analysis of Small 2D Crystals of the Mitochondrial Channel VDAC

“…These images were selected from a much larger data set based on similar (but not identical) lattice parameters and defocus (nominally 1500 to 3000 Å under focus). Each of the correlation averages was obtained by quasi-optical filtration of a VDAC crystal lattice to produce a reference image, which was then used as the search motif against which the crystal field was cross-correlated (e.g., Frank et al, 1988). Lattice areas corresponding to peaks exhibiting correlation (normalized) of at least 0.6 were summed into a "noise-free" correlation average.…”

Outcome of the First Electron Microscopy Validation Task Force Meeting

“…Thus, there are maps for 2D crystal structures that can be obtained using either crystallographic methods (Henderson et al., 1986) or single-particle approaches (Frank et al., 1988); maps for helical structures that can be obtained using either Fourier-Bessel methods (Diaz et al., 2010) or single-particle approaches (Egelman, 2010); single-particle maps, including structures with icosahedral symmetry, other point group symmetries or no symmetry (Rochat and Chiu, 2012); and finally tomogram and sub-tomogram average maps (Schmid and Booth, 2008). …”

Cross-correlation and merging of crystallographic reflections derived from cryoelectron micrographs of 3D crystals: application to the Limulus acrosomal bundle

“…These have shown much potential, and diffraction to better than 3 A A has been reported (Jeng and Chiu, 1983), but this promise has largely gone unfulfilled. Some of the blame may be laid upon the complexity of the packing of the specimen (Frank et al, 1988;Soejima et al, 1993), while some is due to the lack of a good conceptual basis for approaching these specimens.…”