The characterization of some lipid metabolites of Gloeophyllum odoratum grown in vitro

Sterols and fatty acids of different Aspergillus species

“…With all fungi tested, a sterol with M + 376 was present in relatively high amount. A sterol with M + 376 was also observed in other sterol studies of yeasts [16] and basidiomycetes [15]. Comparing mass fragmentation, our results were consistent with the mentioned ones suggesting that the sterol in question was ergosterol peroxide which was very unstable enabling us to detect the molecular ion.…”

“…We tried to identify them by GC/MS. Using available standards (ergosterol, lanosterol and squalene) and data about sterol MS fragmentation obtained by other authors [15–17] we were able to identify squalene and six sterols which all represented intermediates in a principal sterol pathway leading from lanosterol to ergosterol with the exception of ergosta‐8,24(28) dienol. The identified sterols and their relative amounts are presented in Table 1.…”

Medicinal and Aromatic Plants IX

“…The optimal pH level for growth was at about 4.5 to Kahlos et al 1991 ;Kahlos 1996) 5.5 in the solid medium (Figs. The optimal pH level for growth was at about 4.5 to Kahlos et al 1991 ;Kahlos 1996) 5.5 in the solid medium (Figs.…”

“…10). (Data from Kahlos et al 1991;Kahlos 1996) The main free fatty acids were linoleic (CI8:2), or palmitic (C16:0) and stearic (C18:0) acids.…”

“…In the preliminary investigations, the main sterols were ergosterol, ergosta-7,22dien-3ß-ol, fungisterol, ergosterol peroxide in vitro (Table 3; Kahlos et al 1991;Kahlos 1996), which have also been identified as constituents ofwild G. odoratum. As minor or trace compounds (GC-MS analysis) all mycelia occasionally contained cholesterol, ß-sitosterol, stigmasterol, cerevisterol, and some unidentified ergostanes (base peak at m/z 251).…”