The Characterization of New Energy Dependent Cation Transport Processes in Red Blood Cells

Hoffman, Joseph F.; Kregenow, Floyd M.

doi:10.1111/j.1749-6632.1966.tb50182.x

Cited by 204 publications

(106 citation statements)

References 7 publications

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“…Ouabain-inscnsitive sodium efllux includes the movement of sodium both by diffusion and by a recently described active process, "pump II" [5], Our data do not indicate which of these components is increased in MS. This matter is dealt with in greater detail elsewhere [8].…”

Section: Discussioncontrasting

confidence: 36%

Significance of Erythrocyte Sodium Flux in the Pathophysiology and Genetic Expression of Hereditary Spherocytosis

Zipursky

Israels

1971

Pediatr Res

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ExtractErythrocyte sodium transport was studied in five families of patients with hereditary spheroeytosis (HS). Values for ouabain-insensitive sodium efilux in the group with HS (mean = 19.9% of cellular sodium-22/hr, so = ±2.7) were significantly greater (P < 0.001) than those found in the normal group (mean = 13.8%, SD = ±2.7). No clinical evidence of HS was found in the parents; however, 4 of the 10 parents had ouabain-insensitive erythrocyte sodium-22 efilux rates in the HS range in comparison to 3 of 30 control subjects, a statistically significant difference (x ! = 4.67; P < 0.05). Speculation

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Section: Discussioncontrasting

confidence: 36%

Significance of Erythrocyte Sodium Flux in the Pathophysiology and Genetic Expression of Hereditary Spherocytosis

Zipursky

Israels

1971

Pediatr Res

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“…Since ethacrynic acid was used to find whether it could inhibit the sodium-dependent component of sodium efflux and no inhibition was observed when the drug was tested alone, we decided to use it in com-329 D. ERLIJ AND G. LEBLANC bination with strophanthidin as in the experiments described by Hoffman & Kregenow (1966) on human erythrocytes.…”

Section: Resultsmentioning

confidence: 99%

The effects of ethacrynic acid and other sulphydryl reagents on sodium fluxes in frog muscle

Erlij¹,

Leblanc²

1971

The Journal of Physiology

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1. Ethacrynic acid (2 m M) increased the sodium efflux from freshly dissected frog sartorius muscles. This increase was not observed in muscles previously treated with strophanthidin. 2. In strophanthidin‐treated muscles, the addition of ethacrynic acid (2 m M) caused a reduction of sodium efflux. The value of efflux reached in these muscles is similar to that observed in muscles immersed in sodium‐free solutions containing strophanthidin. 3. Ethacrynic acid reduced sodium influx into strophanthidin‐treated muscles. Potassium influx was not affected by this substance. These findings suggest that the inhibitor blocks an exchange of sodium for sodium. 4. The increase in sodium efflux caused by ethacrynic acid did not result from depolarization nor from an increase in [Na]i. 5. Ethacrynic acid caused only a reduction of sodium efflux in muscles previously loaded with sodium by prolonged immersion in potassium‐free solutions at low temperatures. 6. A derivative of ethacrynic acid that lacks the ability to combine with sulphydryl (SH) groups did not reduce sodium efflux from muscles treated with strophanthidin. 7. Para‐chloromercuribenzoic acid (PCMB) reduced sodium efflux from control and strophanthidin‐treated muscles. This reduction seems to be restricted to the sodium dependent component of the efflux. 8. The inhibition of the sodium‐dependent component of sodium efflux caused by ethacrynic acid and PCMB appears to have a similar mechanism, namely, the combination with SH groups.

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“…Of this one-half, 80% is also inhibited by ouabain (O + /E+); however, in the presence of ouabain, ethacrynic acid has an additional effect (O~/E+) which represents about 10% of total outflux. There is some question whether this component is a sodium "pump" [15], or sodium-for-sodium exchange [16].…”

Section: Discussionmentioning

confidence: 99%

“…2). Of the ouabain-insensitive fraction, that portion which was sensitive to ethacrynic acid is energy-dependent [15]. Unlike ouabain-sensitive sodium outflux, this component (O-/E+) did not directly utilize ATP as its energy source [24] and was not affected when potassium was removed from the external medium [15].…”

Section: Discussionmentioning

confidence: 99%

Abnormal Erythrocyte Sodium Transport in Cystic Fibrosis of the Pancreas

Lapey

Gardner

1971

Pediatr Res

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The Characterization of New Energy Dependent Cation Transport Processes in Red Blood Cells

Cited by 204 publications

References 7 publications

Significance of Erythrocyte Sodium Flux in the Pathophysiology and Genetic Expression of Hereditary Spherocytosis

Significance of Erythrocyte Sodium Flux in the Pathophysiology and Genetic Expression of Hereditary Spherocytosis

The effects of ethacrynic acid and other sulphydryl reagents on sodium fluxes in frog muscle

Abnormal Erythrocyte Sodium Transport in Cystic Fibrosis of the Pancreas

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