The characterization of LSD in illicit preparations by pressure-assisted liquid chromatography and gas chromatography

Analysis of ergot alkaloids in flour

“…LC with fluorescence detection is a highly sensitive means of determining lysergic acid diethylamide (LSD), ergotamine, and other ergot alkaloids that possess a 9,10 double bond (Anderson et al, 1978; Christie et al, 1976; Heacock et al, 1973;Jane and Wheals, 1973;Johnson et al, 1977; Perchalski et al, 1975;Twitchett et al, 1978). Minimum detectable quantities of 0.005 ng for LSD (Johnson et al, 1977) and 1 ng for ergotamine (Anderson et al, 1978) have been reported.…”

Determination of dihydroergocryptine in human plasma and urine samples using on-line sample extraction?column-switching reversed-phase liquid chromatography?mass spectrometry

“…Therefore, prior derivatization, e.g., by means of bis(trimethylsilyl) acetamide (BSA) in the reaction port of the gas chromatograph or N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) is required, as described for determination of lysergic acid diethylamide (LSD) [27]. In addition, also planar chromatographic techniques [26] do not meet the basic requirements of both separation efficiency and accuracy.…”

“…For detection of ergot alkaloids measurement of the UV signal responses was applied at 254 and 305 nm [1], 280 nm [8,9,24,25], 300 nm [27], 320 nm [25,26] and despite a reported LOD of 25 ng/ml [25], sensitivity is still expected to be too low for most applications. Therefore, more sensitive alternatives, such as measurement of fluorescence responses [6,14,29,30] in general providing a gain in sensitivity of at least one order of magnitude over UV-detection are required.…”

“…However, liquid chromatographic procedures, coupled to mass spectroscopic detection (MSD), provide some crucial advantages, in particular with concern to specificity. Indeed, high performance liquid chromatographic methods [1,[6][7][8][9]13,[15][16][17][24][25][26][27][28][29][30] prevail in analytical investigations of ergot alkaloids targeted either to get more precise insight into the metabolic pattern or to obtain thorough information of their concentrations in a biological environment, such as, e.g., serum or plasma, urine, tissue, etc. Among them three methods apply the selective "on-line" LC-MS hyphenation [13,15,17] allowing direct and unequivocal structural assignment of the compounds of interest even in those cases where co-elution of two or more species occurs provided ionization energy is not quantitatively consumed by the interfering ones.…”

Biosynthesis of Morphine Alkaloids in Papaver bracteatum Lindl.

“…The Arya I1 variety from western Iran has a particularly high content of thebaine (5 (11)(12)(13)(14)(15)(16)(17). The Arya I1 variety from western Iran has a particularly high content of thebaine (5 (11)(12)(13)(14)(15)(16)(17).…”