The Characterization of L. plantarum-fermented Opuntia humifusa

Abstract

To increase the bioavailability of Opuntia humifusa, O. humifusa was fermented using L.plantarum, and the characteristics of the fermented extract were confirmed. The characteristics of fermented extracts were investigated through viscosity, total polyphenol content, amino acid, antibacterial activity, cytokine analysis and zymography. The viscosity decreased by half and vitamin C remained almost unchanged during fermentation. Total polyphenols, most amino acids, total amino acids and major minerals were increased by fermentation. There was no cytotoxicity, and the activity of cytokines and MMP-9 in inflammation was inhibited. The inhibitory effect on inflammation and tissue destruction was found to be inhibited in L. plantarum fermentation extracts compared to hot water extracts. These results are expected to be the basis for the development of materials that enhance the functionality of L.plantarum-fermented O. humifusa extract.•

Cytotoxicity, metabolic enzyme inhibitory, and anti‐inflammatory effect of Lentinula edodes fermented using probiotic lactobacteria

“…Several studies have shown that consumption of probiotics containing Bifidobacterium lactis HN019 (Chiang et al., 2000), L. rhamnosus GG (Gorbach et al., 1987), and L. casei (Aso et al., 1995) enhanced natural cellular immunity by increasing circulating lymphoid cells and protecting against gastrointestinal infection and tumor growth. A previous study (Jung et al., 2017) showed that L. plantarum fermentation enhanced the anti‐inflammatory effects (inhibition of TNF‐α and MMP‐9 production) of plant food‐material extract, and the antibacterial activity of this single‐strain ferment was remarkably stronger than that of two mixed strains ( L. plantarum and L. rhamnosus GG). By fermentation of L. brevis LB‐20 (Song et al., 2011), DPPH radical‐scavenging activity increased from 39%–49% to 53%–68%, hydroxyl radical‐scavenging activity increased from 48%–64% to 61%–79%, and it was confirmed that the superoxide or alkyl radical‐scavenging ability was also increased.…”