The Characterization of Intracellular and Extracellular Biomineralization Induced by<i>Synechocystis sp</i>. PCC6803 Cultured under Low Mg/Ca Ratios Conditions

Han, Zuozhen; Zhao, Yanyang; Yan, Huaxiao; Zhao, Hui; Han, Mei; Sun, Bin; Meng, Ruirui; Zhuang, Dingxiang; Li, Dan; Gao, Wenjing; Du, Shiyun; Wang, Xiaoai; Fan, Kaixiang; Hu, Wenya; Zhang, Mengxue

doi:10.1080/01490451.2016.1197986

Cited by 31 publications

(26 citation statements)

References 26 publications

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“…The results of this experiment demonstrated that the presence of intracellular inclusions containing Ca and Mg in SRB2 bacteria also contributed to the decrease in Ca 2+ and Mg 2+ ions concentrations. In fact, for many microorganisms, extracellular and intracellular biomineralization can occur at the same time [23,45,49,72]. It has been found that many organelles, even nucleic acid, can be involved in the intracellular biomineralization process [73].…”

Section: Intracellular Amorphous Inclusionsmentioning

confidence: 99%

“…The definition of biomineralization is that microorganisms can change the local environment surrounding cells by increasing the pH and elevating the supersaturation to induce the precipitation of minerals [23][24][25]. Biomineralization can be divided into two types: biologically controlled mineralization (BCM) and biologically induced mineralization (BIM) based on the degree of biological control [26].…”

Section: Introductionmentioning

confidence: 99%

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Bio-Precipitation of Calcium and Magnesium Ions through Extracellular and Intracellular Process Induced by Bacillus Licheniformis SRB2

et al. 2019

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Removal of calcium and magnesium ions through biomineralization induced by bacteria has been proven to be an effective and environmentally friendly method to improve water quality, but the process and mechanism are far from fully understood. In this study, a newly isolated probiotic Bacillus licheniformis SRB2 (GenBank: KM884945.1) was used to induce the bio-precipitation of calcium and magnesium at various Mg/Ca molar ratios (0, 6, 8, 10, and 12) in medium with 30 g L−1 sodium chloride. Due to the increasing pH and HCO3− and CO32− concentrations caused by NH3 and carbonic anhydrase, about 98% Ca2+ and 50% Mg2+ were precipitated in 12 days. The pathways of bio-precipitation include extracellular and intracellular processes. Biominerals with more negative δ13C values (−16‰ to −18‰) were formed including calcite, vaterite, monohydrocalcite, and nesquehonite with preferred orientation. The nucleation on extracellular polymeric substances was controlled by the negatively charged amino acids and organic functional groups. The intracellular amorphous inclusions containing calcium and magnesium also contributed to the bio-precipitation. This study reveals the process and mechanism of microbial desalination for the removal of calcium and magnesium, and provides some references to explain the formation of the nesquehonite and other carbonate minerals in a natural and ancient earth surface environment.

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Section: Intracellular Amorphous Inclusionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bio-Precipitation of Calcium and Magnesium Ions through Extracellular and Intracellular Process Induced by Bacillus Licheniformis SRB2

et al. 2019

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“…Microorganisms, especially bacteria, as an important part of the Earth's biosphere, can facilitate many geochemical processes. Extensive research has been undertaken on the biomineralization processes of carbonate and phosphate minerals in the past decades, and the significant roles that microorganisms play in these processes have been widely reported [1][2][3][4][5][6][7][8][9][10][11]. Many species of microorganisms have been used to induce the precipitation of carbonate and phosphate minerals, including cyanobacteria, halophiles, methanogens and sulfate-reducing bacteria [8,[11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Sun

Zhao

et al. 2020

Minerals

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In this study, a facultative anaerobic strain isolated from marine sediments and identified as Citrobacter freundii, was used to induce the precipitation of carbonate and phosphate minerals in the laboratory under anaerobic conditions. This is the first time that the ability of C. freundii ZW123 to precipitate carbonate and phosphate minerals has been demonstrated. During the experiments, carbonic anhydrase, alkaline phosphatase and ammonium released by the bacteria not only promoted an increase in pH, but also drove the supersaturation and precipitation of carbonate and phosphate minerals. The predominant bio-mediated minerals precipitated at various Mg/Ca molar ratios were calcite, vaterite, Mg-rich calcite, monohydrocalcite and struvite. A preferred orientation towards struvite was observed. Scanning transmission electron microscopy (STEM) and elemental mapping showed the distribution of magnesium and calcium elements within Mg-rich calcite. Many organic functional groups, including C=O, C–O–C and C–O, were detected within the biominerals, and these functional groups were also identified in the associated extracellular polymeric substances (EPS). Fifteen kinds of amino acid were detected in the biotic minerals, almost identical to those of the EPS, indicating a close relationship between EPS and biominerals. Most amino acids are negatively charged and able to adsorb cations, providing an oversaturated microenvironment to facilitate mineral nucleation. The X-ray photoelectron spectroscopy (XPS) spectrum of struvite shows the presence of organic functional groups on the mineral surface, suggesting a role of the microorganism in struvite precipitation. The ZW123 bacteria provided carbon and nitrogen for the formation of the biotic minerals through their metabolism, which further emphasizes the close relationship between biominerals and the microorganisms. Thermal studies showed the enhanced thermal stability of biotic minerals, perhaps due to the participation of the bacteria ZW123. The presence of amino acids such as Asp and Glu may explain the high magnesium content of some calcites. Molecular dynamics simulations demonstrated that the morphological change and preferred orientation were likely caused by selective adsorption of EPS onto the various struvite crystal surfaces. Thus, this study shows the significant role played by C. freundii ZW123 in the bioprecipitation of carbonate and phosphate minerals and provides some insights into the processes involved.

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“…CA is a ubiquitous metalloenzyme existed in organism which can catalyze the reversible hydration reaction of CO2 [18]. CA can eliminate the dynamic obstacles and plays an important catalytic role to promote the inorganic carbon conversion reaction [42]. The conversion between CO2 and HCO3 -can be catalyzed by this enzyme and the conversion formula is as follows:…”

Section: The Biological Activity Of Bacteria Affects Biomineralizationmentioning

confidence: 99%

Biomineralization of Carbonate Minerals Induced by Halophilic <em>Chromohalobacter israelensis</em> in High Salt Concentration: Implications for Natural Environments

Han

Li²,

Zhao

et al. 2017

Preprint

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High salt environment was widespread in modern and geological record, and sedimentation induced by microbes in these systems was an important part of sedimentary minerals and rocks. The mechanism of microbiologically induced carbonate precipitation has not been solved thoroughly although numerous scholars and experts have made specifically research of the problems with respect to minerals induced by bacteria. The study of carbonate minerals induced by Halophilic bacteria has aroused wide concern. The present study was aim to investigate the characterization and process of biomineralization in the high salt system, a Halophilic bacterium, Chromohalobacter israelensis LD532 strain (Genbank: KX766026), which isolated from Yinjiashan Saltern of China, was selected as an object to induce carbonate minerals. Carbonate minerals induced by LD532 were investigated in several comparative experimental sets with Mg resources of magnesium sulfate and magnesium chloride. Magnesium calcite and aragonite were induced by LD532 bacteria while these minerals were not in the control group. The mineral phases, micromorphologies, and crystal structures were analyzed using X-ray powder diffraction, scanning electron microscope, and energy dispersive X-ray detector. Carbonic anhydrase and urease secreted by strain LD532 through metabolism promoted the pH values of the liquid medium and the process of carbonate precipitation. Further study proved that the nucleation sites of partial carbonate nucleus were located on the extracellular polymeric substance and the membrane of intracellular vesicles of LD532 bacteria by high resolution transmission electron microscopy, energy dispersive X-ray detector and ultrathin slices analysis, which provided favorable conditions for the growth of carbonate mineral crystals. The morphology and composition of minerals formed in MgSO4 and MgCl2 solution have significant differences, indicating that different sources of Mg 2+ could also affect the physiological and biochemical activities of microorganisms and then affect the mineral deposition. The accomplished study is of certain interest for interpretation of the carbonates biomineraliazation in natural salt environment, and has a certain reference value in understand of the sedimentary carbonates in ancient marine environment like evaporated tidal flat.

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The Characterization of Intracellular and Extracellular Biomineralization Induced bySynechocystis sp. PCC6803 Cultured under Low Mg/Ca Ratios Conditions

Cited by 31 publications

References 26 publications

Bio-Precipitation of Calcium and Magnesium Ions through Extracellular and Intracellular Process Induced by Bacillus Licheniformis SRB2

Bio-Precipitation of Calcium and Magnesium Ions through Extracellular and Intracellular Process Induced by Bacillus Licheniformis SRB2

Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Biomineralization of Carbonate Minerals Induced by Halophilic <em>Chromohalobacter israelensis</em> in High Salt Concentration: Implications for Natural Environments

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