The characterization of heterogeneous antibody-hapten interactions using non-linear regression analysis on fluorescence quenching curves

Flanagan, Michael T.; Tattam, Frederick G.; Green, N. Michael

doi:10.1016/0161-5890(78)90064-0

Cited by 10 publications

(3 citation statements)

References 24 publications

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“…Although there have been numerous elegant attempts to modify carbon surfaces, it has been found that improvements in voltammetric behavior can be obtained by simple pretreatment of the electrode surface. Pretreatments that have been useful include high temperatures (2), electrochemical anodization (3)(4)(5)(6)(7)(8), and high-speed polishing methods (9-10). These treatments are efficacious at several forms of carbon such as glassy carbon, carbon paste, and carbon fibers (1).…”

Section: Discussionmentioning

confidence: 99%

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Application of integral pk spectrometry to the titration curve of fulvic acid

Leuenberger¹,

Schindler²

1986

Anal. Chem.

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Section: Discussionmentioning

confidence: 99%

“…It is important to note that the technique of pK spectrometry is by no means restricted to the analysis of acid-base titration data. The same method can be used to analyze any kind of association-dissociation equilibria (1)(2)(3)(4)(5)(6)(7)(8).…”

mentioning

confidence: 99%

Application of integral pk spectrometry to the titration curve of fulvic acid

Leuenberger¹,

Schindler²

1986

Anal. Chem.

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“…This and the subsystem results indicate the importance of good initial estimates. Flanagan et al (1978) found, for simpler multiple antibody, single hapten equilibria, that starting with the lowest analyte concentration, making all initial complex concentration estimates zero and then using the results for each analyte concentration the estimates for the next highest analyte concentration, worked well. No such simple technique could be found that would work with the competitive assay.…”

Section: Classical Regressionmentioning

confidence: 96%

Modelling of the immunoassay element of an optical immunosensor

Keating

Flanagan²

2001

Physiol. Meas.

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Several factors affect the viability of biosensor design. A computer-based model is being developed to enable the sources and effect of noise and variability within the sensor to be analysed. The work now presented details the modelling of the biochemical aspect of the biosensor model-the immunoassay. The equilibrium equations that describe the chemical reactions that occur when a sample containing the analyte is added to the immunosensor are cast as a sum of squares function that can be minimized using an optimization procedure. The optimization returns the concentrations of each species at equilibrium and the procedure is incorporated within a Monte Carlo simulation, which allows the variations in the resulting concentrations to be determined. Three classes of optimization technique are considered, classical regression techniques and two intelligent optimization techniques: simulated annealing and genetic algorithms. Several methods of imposing constraints are implemented and the issue of local minima is discussed. Classical regression procedures were found to be superior to the intelligent optimizations examined.

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Thin‐film antimony–antimony‐oxide enzyme electrode for penicillin determination

Flanagan

Carroll

1986

Biotech & Bioengineering

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A potentiometric penicillinase electrode is reported in which the base pH transducer is a thin-film antimony-antimony-oxide electrode deposited by vacuum evaporation. Several enzyme immobilization procedures have been examined and a crosslinked protein film found to be the most appropriate to this type of sensor. The use of an adjacent antimony-antimony-oxide track as a pseudoreference electrode was successfully demonstrated. The overall response was shown to be independent of the stirring rate above 100 rpm, but the kinetics of the response were found to depend markedly on the stirring rate. The intrinsic linear response range was 3 x 10(-4)M to 7 x 10(-3)M penicillin G. Linearizing transforms that extend the useful range were examined.

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The characterization of heterogeneous antibody-hapten interactions using non-linear regression analysis on fluorescence quenching curves

Cited by 10 publications

References 24 publications

Application of integral pk spectrometry to the titration curve of fulvic acid

Application of integral pk spectrometry to the titration curve of fulvic acid

Modelling of the immunoassay element of an optical immunosensor

Thin‐film antimony–antimony‐oxide enzyme electrode for penicillin determination

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