The Characterization of Bovine Bone-Derived Hydroxyapatite Isolated Using Novel Non-Hazardous Method

Abstract

Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800°C, 900°C and 1,000°C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 ± 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.

Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect

“…is is possibly because BHA-GEL as the main material for the matrix resembles the inorganic and organic components of bone. Because of this, the implants may also function as bone tissue scaffold and make the growth of blood vessels in the damaged tissue faster than the negative control group [4,11,12].…”

“…Bovine hydroxyapatite (BHA) was extracted from bovine bone at temperature of 1000°C based on the procedure of the previous study [4]. A total of 10 grams of BHA, 5 ml 20 wt% gelatin (GEL, Cartino, ailand), and 10% gentamicin (GEN, Yantai Justaware Pharmaceutical, China) are mixed until reaching homogeneous mixture to form a mass that can be made as granule.…”

“…For example, it is synthesized from calcium and phosphate or directly extracted from natural sources such as bovine bone. Hydroxyapatite from bovine bone is called bovine hydroxyapatite (BHA) [4]. One of the differences between BHA and synthetic hydroxyapatite is that BHA has a carbonate group that increases osteoblast proliferation and differentiation, thereby making bone matrix synthesis faster [5,6].…”

Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial

“…Hydroxyapatite (HA) is a biomaterial used for the production of orthopedic and dental implants. Typically, HA is used as a single material or composite in combination with other materials [1][2][3], a coating agent for bone graft [1,4,5], and a matrix for drug delivery systems targeting bone tissue [6]. HA is widely used because it contains similar physical and chemical components to those of natural HA present in bone tissue [2].…”

“…Typically, HA is used as a single material or composite in combination with other materials [1][2][3], a coating agent for bone graft [1,4,5], and a matrix for drug delivery systems targeting bone tissue [6]. HA is widely used because it contains similar physical and chemical components to those of natural HA present in bone tissue [2]. Moreover, studies reported that HA induced a favorable immune response [7], exerted an angiogenic effect on defective bone tissue [8,9], and activated osteoblasts and osteoclasts for bone tissue remodeling by accelerating the differentiation of these cells [10,11].…”